Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 951,882 T→C 7.5% L238S (TTG→TCG)  cyoB → cytochrome bo terminal oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947951,8820TC 7.5% 152.6 / 2.3 53L238S (TTG→TCG) cyoBcytochrome bo terminal oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base T (27/22);  new base C (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

ATCTGGGCGCTACAGCTATCCGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCTGGGCGTGGGGTCACCCTGAGGT  >  NC_002947/951740‑952029
                                                                                                                                              |                                                                                                                                                   
aTCTGGGCGCTACAGCTATCCGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGAtcgt                                                                                                                                               >  6:261603/1‑149 (MQ=255)
aTCTGGGCGCTACAGCTATCCGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGAtcgt                                                                                                                                               >  4:162593/1‑149 (MQ=255)
   tGGGCGCTACAGCTATCCGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGc                                                                                                                                            <  5:261603/149‑1 (MQ=255)
     ggCGCTACAGCTATCCGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGAtcgtc                                                                                                                                              <  6:71702/145‑1 (MQ=255)
      gcgcTACAGCTATCCGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTc                                                                                                                                         <  3:12415/149‑1 (MQ=255)
          tACAGCTATCCGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTc                                                                                                                                     >  2:17962/1‑149 (MQ=255)
          tACAGCTATCCGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTCGATCGTCGCTTCGTTc                                                                                                                                     >  7:116243/1‑149 (MQ=255)
                  tCCGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCt                                                                                                                             <  8:156277/149‑1 (MQ=255)
                    cGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGa                                                                                                                           <  1:17962/149‑1 (MQ=255)
                    cGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGa                                                                                                                           <  3:162593/149‑1 (MQ=255)
                            gTACGACGCTTACTGGCGTGAACTTCCTCGTCACCTTGATGAAGATGCGAGCACCTGGCATGAAACTGATGGACATTACGATCTTCACATGGAAATGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCa                                                                                                                   >  7:126004/1‑149 (MQ=255)
                             tACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCTTCGATTCGTTCCCTATCCTGACTGCTGCAc                                                                                                                  >  8:276439/1‑149 (MQ=255)
                                gacgCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCg                                                                                                               >  6:262288/1‑149 (MQ=255)
                                     ttACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGGCCCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATACTGACTGCTGCGCTCGCTctg                                                                                                          >  8:172357/1‑149 (MQ=255)
                                           gCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACt                                                                                                    <  5:296753/149‑1 (MQ=255)
                                              tGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGtt                                                                                                 <  5:262288/149‑1 (MQ=255)
                                                 aCTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGAc                                                                                              >  3:307501/1‑149 (MQ=255)
                                                  cTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGAcc                                                                                             >  8:210107/1‑149 (MQ=255)
                                                   ttCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCg                                                                                                               >  5:262823/1‑130 (MQ=255)
                                                   ttCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCg                                                                                                               <  6:262823/130‑1 (MQ=255)
                                                   ttCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCg                                                                                            >  3:108593/1‑149 (MQ=255)
                                                   gtCCTCGTCACCCGGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACCGTTGACCg                                                                                            <  2:364527/148‑1 (MQ=255)
                                                      cTCGTACCCGTTATGAAGATGCGCGCACCTGGCATGAAACTGATGGAGATTCCTATCTTCACATGTACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGtt                                                                                          <  8:126004/148‑1 (MQ=255)
                                                       tCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGAc                                                                                                     >  5:301508/1‑136 (MQ=255)
                                                       tCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGAc                                                                                                     <  6:301508/136‑1 (MQ=255)
                                                       tCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTAt                                                                                        >  8:237933/1‑149 (MQ=255)
                                                          tCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTg                                                                                     <  4:108593/149‑1 (MQ=255)
                                                          tCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTCGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTg                                                                                     >  8:82076/1‑149 (MQ=255)
                                                             ccGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGAc                                                                                  <  7:210107/149‑1 (MQ=255)
                                                                        aTGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTCGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCtt                                                                       <  8:116243/149‑1 (MQ=255)
                                                                            gcgcACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCAc                                                                    >  4:224716/1‑148 (MQ=255)
                                                                                aCCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTctgct                                                                                                        <  8:216313/108‑1 (MQ=255)
                                                                                aCCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTctgct                                                                                                        >  7:216313/1‑108 (MQ=255)
                                                                                        tGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTtggt                                                       <  3:224716/149‑1 (MQ=255)
                                                                                        tGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTCGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTtggt                                                       <  7:82076/149‑1 (MQ=255)
                                                                                           aaCTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTtggt                                                       <  2:367647/146‑1 (MQ=255)
                                                                                           aaCTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTAACTGGACTTCCACATCTTCACCAACGAGCTtggt                                                       >  1:367647/1‑146 (MQ=255)
                                                                                            aCTGATGCACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTTCACATCCTCACCAACGAGCTTGGTggg                                                    >  2:242301/1‑148 (MQ=255)
                                                                                                  ggACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACt                                                                                                    <  8:77382/94‑1 (MQ=255)
                                                                                                  ggACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACt                                                                                                    >  7:77382/1‑94 (MQ=255)
                                                                                                    aCATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTCCACTCGCTCTCCTGACTGTTGACCGATATCTGGACTTCCACATCTTCACCAACGACCATGCTGGGAACCCgat                                            >  5:126696/1‑148 (MQ=255)
                                                                                                        gCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGatgatg                                        >  3:172058/1‑148 (MQ=255)
                                                                                                        gCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGatgatg                                        >  4:89163/1‑148 (MQ=255)
                                                                                                            aTCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGt                                   >  2:139170/1‑149 (MQ=255)
                                                                                                                       gACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCt                        <  2:115382/149‑1 (MQ=255)
                                                                                                                         ccTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCTgg                      <  4:307501/149‑1 (MQ=255)
                                                                                                                             cACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCTGGGCGt                  >  4:85285/1‑149 (MQ=255)
                                                                                                                                      cAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAAc                                                                >  6:314502/1‑94 (MQ=255)
                                                                                                                                      cAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAAc                                                                <  5:314502/94‑1 (MQ=255)
                                                                                                                                       aaCGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTgg                                                                                    <  1:305693/73‑1 (MQ=255)
                                                                                                                                       aaCGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTgg                                                                                    >  2:305693/1‑73 (MQ=255)
                                                                                                                                       aaCGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCTGGGCGTGGGGTCAccc        >  7:325629/1‑149 (MQ=255)
                                                                                                                                            gtTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCTGGGCGTGGGGTCACCCTGAgg   >  3:153058/1‑149 (MQ=255)
                                                                                                                                             ttGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCTGGGCGTGGGGTc            >  7:351653/1‑139 (MQ=255)
                                                                                                                                             ttGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCTGGGCGTGGGGTc            <  8:351653/139‑1 (MQ=255)
                                                                                                                                             ttGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCTGGGCGTGGGGTCACCCTGAGGt  >  2:262526/1‑149 (MQ=255)
                                                                                                                                             ttGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCTGGGCGTGGGGTCACCCTGAGGt  <  3:85285/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
ATCTGGGCGCTACAGCTATCCGGATTGGGTACGACGCTTACTGGCGTGAACTTCCTCGTCACCGTGATGAAGATGCGCGCACCTGGCATGAAACTGATGGACATGCCGATCTTCACCTGGACCTGCACCTGGGCCAACGTGTTGATCGTCGCTTCGTTCCCGATCCTGACTGCTGCACTCGCTCTGCTGACTGTTGACCGTTATCTGGACTTCCACATCTTCACCAACGAGCTTGGTGGGAACCCGATGATGTACGTCAACCTGTTCTGGGCGTGGGGTCACCCTGAGGT  >  NC_002947/951740‑952029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑