| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,013,249 | T→G | 14.7% | intergenic (+77/+54) | prfC → / ← potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,013,249 | 0 | T | G | 14.7% | 127.9 / 15.8 | 54 | intergenic (+77/+54) | prfC/potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (25/20); new base G (4/4); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01 | |||||||||||
GGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCAT > NC_002947/1013102‑1013392 | gtgTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtg < 7:275858/147‑1 (MQ=255) ggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt < 1:273526/149‑1 (MQ=255) aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGAt < 2:324876/148‑1 (MQ=255) aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCGCTACCACAGGAATCACCAGGCCTGGAGTTGATGTGATag > 5:275697/1‑148 (MQ=39) cTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACATGACCTCCAGGTCTGGTGATTCCt < 3:100521/149‑1 (MQ=25) gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgtg < 6:253415/149‑1 (MQ=25) gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgtg < 3:158345/149‑1 (MQ=255) cgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg < 8:131224/149‑1 (MQ=255) gAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAACTCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGc < 1:41935/149‑1 (MQ=21) gagCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAGGAATCACCAGACCTGGAGGTCATGTGGTAACTGTGGGAGCGGGCGTg > 7:307568/1‑149 (MQ=255) gcgcTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGGCATGTGAGACCTGTGGGAGCGGGCGTGcc > 7:82488/1‑149 (MQ=255) cTGGCCGTACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCcgc < 7:31210/149‑1 (MQ=255) cTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgtg > 2:342830/1‑132 (MQ=21) cTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgtg < 1:342830/132‑1 (MQ=21) tGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCACCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCcgc > 3:146298/1‑148 (MQ=255) tCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCGGGAGGTCATGTGATACGTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCt > 2:54727/1‑149 (MQ=255) ttCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCACCCCCAGGAATCACCAGACCGGGAGGGCATGTGATACCGGTGGGTGCGGGCGTGCCCCCGAACG‑GCCGCTAggcg > 8:29025/1‑149 (MQ=38) ttCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc > 6:108156/1‑111 (MQ=255) ttCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc < 5:108156/111‑1 (MQ=255) ttCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAggcg > 3:279548/1‑149 (MQ=255) cccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTc < 4:279548/149‑1 (MQ=255) aCCATAAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCgg < 7:29025/149‑1 (MQ=255) ccATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGc > 7:283993/1‑149 (MQ=255) aGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGAGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTg < 1:54727/148‑1 (MQ=255) aGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGGCCCATTTCAATTTACGGCTTTGa > 7:184659/1‑148 (MQ=255) gCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGCCCGGGAGGGCATGTGATACCTGTGGGCGCGGGCGTGCCCGCGCAGG‑GCCGCTAGGCGGCCCCCTTTGAATTTCCGGCGTTGGTAc > 1:317418/1‑149 (MQ=25) cccTCAACTCGGGGCTGCTTTCCCGCCCTTCGCGGGCCCGCCGGCCCCCACCGCAACCCCCACCCCTGGCGGTCATGTTATACCTGGGGGTGGGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATAc < 6:14214/148‑1 (MQ=255) cttaattCGGGGCTGCTTGGCAGCCCTTCGCGGGCCCGCCCGCTCCCACCGCAATCCCCAGACCTGGAGGTCTTGTGATACGTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTg < 4:146298/143‑1 (MQ=25) gAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCGTTGATACTGGt > 5:144580/1‑149 (MQ=255) aacgcGGGGCGGCTTTGCAGCCCTGCGCGGGCCCGCCCGCTCCCCCCGCAACCCCCCGCCCTGGCGGTCATGTGATACCTGTGGGAGCGGGCGGGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTc < 2:317418/145‑1 (MQ=255) ggggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGa < 8:283993/148‑1 (MQ=255) ggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGGATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTc > 5:105676/1‑149 (MQ=255) tgctTTGCAGCCCTTCGCGGGCACGCCCCCGCCCACAGGGATAACCAGACCGGGCGGTGCAGTGATTACGGTGGGGGCGGGCGTGGGCGCGAAGG‑GCCGCGAGGCGGGCCCCGGTCAATTTGCGGCGTTTATGCGGGTGCAGAATcggg > 8:348826/1‑147 (MQ=12) gCCCTTCGCGGGCACGCCCTCTCCCACCGGAATCACCAGACCTGGAGTTCATTTGATACCTTTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATAc < 8:66237/149‑1 (MQ=38) cgcgGGCCCGCCCGCTCCCACCGGAATCACCCGACATGGAGGTCATGTTATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGa < 2:214277/149‑1 (MQ=38) gcgGGCACGCCCGCTCCCACAGGAAGCACCAGACCTGGAGGTAATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGa < 8:82488/148‑1 (MQ=255) gaaCGCCGGGTCCCACAGGATTAACCAGACGTGGAGGTCATGTGATACGTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATTCTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc < 3:148507/147‑1 (MQ=38) gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg > 1:12996/1‑149 (MQ=255) ccACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCTGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc > 1:108422/1‑149 (MQ=21) cacaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt > 8:194357/1‑149 (MQ=255) acaGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc > 8:9645/1‑149 (MQ=25) acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc > 3:317547/1‑149 (MQ=255) acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc > 7:24649/1‑149 (MQ=255) gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa > 1:107767/1‑147 (MQ=255) gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa < 2:107767/147‑1 (MQ=255) gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc > 8:108602/1‑149 (MQ=255) gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc > 2:246828/1‑149 (MQ=255) gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc > 2:289347/1‑149 (MQ=255) cACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCAt < 7:108602/149‑1 (MQ=255) acatgACCTCCAGGTCTGGTGATTCCTGTGGCACCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCGCCAATTCAATTTCCGGCATTGATACAGGACCAGATTCCCCTACGAAAACAGTCGATCTTCGGCGGCATCGCCTCCAGCGCAt > 7:134346/5‑148 (MQ=18) aCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCAt > 6:197137/1‑148 (MQ=255) aCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa < 5:152284/149‑1 (MQ=255) aCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa > 6:152284/1‑149 (MQ=255) ccAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa > 5:379303/1‑148 (MQ=255) cTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTg > 7:44826/1‑149 (MQ=255) gTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCAt < 6:105676/148‑1 (MQ=255) | GGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑GCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCAT > NC_002947/1013102‑1013392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |