Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,017,834 C→T 8.1% G10S (GGT→AGT)  PP_5461 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,017,8340CT 8.1% 213.7 / 6.4 74G10S (GGT→AGT) PP_5461hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (39/29);  new base T (3/3);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.01e-01

GCGTCTGCAGTCAGCAAGCAGGTATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCTCAATGGCAAC  >  NC_002947/1017686‑1017982
                                                                                                                                                    |                                                                                                                                                    
gCGTCTGCAGTCAGCAAGCAGGTATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATACTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGAAGAAACCGGCGAAGGCCATGGTGTCCAcc                                                                                                                                                      >  5:281182/1‑149 (MQ=255)
       cAGTCAGCAAGCAGGTATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTaaaa                                                                                                                                                 <  3:361832/147‑1 (MQ=255)
        aGTCAGCAAGCAGGTATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTg                                                                                                                                              >  7:114706/1‑149 (MQ=255)
              caagcaGGTATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTaa                                                                                                                                                   >  5:234064/1‑138 (MQ=255)
              caagcaGGTATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGacac                                                                                                                                         >  5:281384/1‑148 (MQ=255)
              caagcaGGTATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGGCCACCTaa                                                                                                                                                   <  6:234064/138‑1 (MQ=255)
                 gcaGGTATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACTTTTAGGTGGACACCAtg                                                                                                                                     <  7:24318/149‑2 (MQ=255)
                 gcaGGTATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACTTTTAGGTGGACACCAtg                                                                                                                                     <  7:21913/149‑2 (MQ=255)
                      tATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTgg                                                                                                                                <  2:27875/149‑1 (MQ=255)
                        tCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGc                                                                                                                               <  6:281182/148‑1 (MQ=255)
                          aaaGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGccc                                                                                                                             <  5:202841/148‑1 (MQ=255)
                            aGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATc                                                                                                                          <  8:266961/149‑1 (MQ=255)
                              cATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATc                                                                                                                          >  1:213523/1‑147 (MQ=255)
                               aTTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTgg                                                                                                                                <  4:354750/140‑1 (MQ=255)
                               aTTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTgg                                                                                                                                >  3:354750/1‑140 (MQ=255)
                                   aaaaTGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGa                                                                                                                                            <  5:236097/124‑1 (MQ=255)
                                   aaaaTGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGa                                                                                                                                            >  6:236097/1‑124 (MQ=255)
                                   aaaaTGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGa                                                                                                                   >  4:210000/1‑149 (MQ=255)
                                      aTGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGAttt                                                                                                                <  3:210000/149‑1 (MQ=255)
                                           ttGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAg                                                                                                           >  7:193174/1‑149 (MQ=255)
                                              tCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGc                                                                                                         <  2:213523/148‑1 (MQ=255)
                                                ccGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATATCTGGCCCAGCAAGCGGATTTTCCAGGCATc                                                                                                      >  5:212904/1‑149 (MQ=255)
                                                        aaaaaaaCTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAgcgc                                                                                              >  8:1993/1‑149 (MQ=255)
                                                                    acTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACaa                                                                                   >  2:230625/1‑148 (MQ=255)
                                                                     cTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATa                                                                                 <  7:1993/149‑1 (MQ=255)
                                                                         ccTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAAcc                                                                             >  3:151103/1‑149 (MQ=255)
                                                                         ccTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAAcc                                                                             >  1:2638/1‑149 (MQ=255)
                                                                         ccTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAAcc                                                                             >  7:338529/1‑149 (MQ=255)
                                                                          cTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAACCCt                                                                            >  4:311427/1‑149 (MQ=255)
                                                                             aTAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCtt                                                                           <  2:2638/147‑1 (MQ=255)
                                                                              tAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTc                                                                         >  4:144759/1‑148 (MQ=255)
                                                                                gTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCaaa                                                                      <  8:114706/149‑1 (MQ=255)
                                                                                gTCGAATCCCGGTATACGTCCGGCCAACAAATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAATTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCaaa                                                                      <  8:234697/149‑1 (MQ=255)
                                                                                 tCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAg                                                                     >  2:61471/1‑149 (MQ=255)
                                                                                      tCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGcc                                                                   >  3:26465/1‑146 (MQ=255)
                                                                                         cGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGt                                                             <  3:144759/149‑1 (MQ=255)
                                                                                         cGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCCAACGCCAAGCTCTCCACCTAAAACTGCACACCATAACTCGCCCATCAAGCGGATTATCCAGGCATCCTCAGCGCCGTACCTACAAAAAACCTTTCAAAGCCCagact                                                             >  7:118388/1‑147 (MQ=255)
                                                                                          ggTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTg                                                            <  4:151103/149‑1 (MQ=255)
                                                                                           gTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGc                                                           >  1:167388/1‑149 (MQ=255)
                                                                                               ccgtccgGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGcgac                                                        >  4:254897/1‑148 (MQ=255)
                                                                                                cgtccgGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGcgac                                                        >  6:53383/1‑147 (MQ=255)
                                                                                                 gtccgGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAg                                                     >  6:366525/1‑149 (MQ=255)
                                                                                                    ctgCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGccc                                                  >  6:87543/3‑149 (MQ=255)
                                                                                                      gCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATaaa                                                                               <  7:73863/118‑1 (MQ=255)
                                                                                                      gCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATaaa                                                                               >  8:73863/1‑118 (MQ=255)
                                                                                                        cAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTATAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGgcg                                              >  6:171662/1‑149 (MQ=255)
                                                                                                         aaCACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGgcgc                                             <  3:254897/149‑1 (MQ=255)
                                                                                                            acaTCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTc                                          >  2:151799/1‑149 (MQ=255)
                                                                                                              aTCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCa                                        >  5:144959/1‑149 (MQ=255)
                                                                                                                 ttCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCg                                     >  1:162224/1‑149 (MQ=255)
                                                                                                                  tCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGgc                                               <  4:34268/138‑1 (MQ=255)
                                                                                                                  tCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGgc                                               >  3:34268/1‑138 (MQ=255)
                                                                                                                     tGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCaaa                                 >  4:29955/1‑149 (MQ=255)
                                                                                                                       aCGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACa                               >  7:208633/1‑149 (MQ=255)
                                                                                                                        cGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACAt                              >  1:289110/1‑149 (MQ=255)
                                                                                                                         gAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATc                             >  7:346993/1‑149 (MQ=255)
                                                                                                                          aaaCCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCg                            <  3:29955/149‑1 (MQ=255)
                                                                                                                                gCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTgg                      <  4:26465/149‑1 (MQ=255)
                                                                                                                                   aaGGCCATGGTGTCCACCTAAAAGAGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGcc                   <  6:212904/149‑1 (MQ=255)
                                                                                                                                       ccATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCCAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCcaca                <  8:338529/148‑1 (MQ=255)
                                                                                                                                       ccATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAg               >  1:121095/1‑149 (MQ=255)
                                                                                                                                           ggTGTCCACTTTTAGGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCt            >  4:201484/1‑148 (MQ=255)
                                                                                                                                            gtgtCCACTTTTAGGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCt                                           >  6:380570/1‑116 (MQ=255)
                                                                                                                                            gtgtCCACTTTTAGGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCt                                           <  5:380570/116‑1 (MQ=255)
                                                                                                                                            gtgtCCACCTAAAATTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGc                    <  2:140005/139‑1 (MQ=255)
                                                                                                                                            gtgtCCACCTAAAATTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGc                    >  1:140005/1‑139 (MQ=255)
                                                                                                                                             tgtCCACTTTTAGGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCCCAAACATCGCCTTGGGCCACAGCCTCaa         >  4:91123/1‑149 (MQ=255)
                                                                                                                                             tgtCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGGGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTg                       >  8:129111/1‑135 (MQ=255)
                                                                                                                                             tgtCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGGGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTg                       <  7:129111/135‑1 (MQ=255)
                                                                                                                                             tgtCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCTCaa         <  5:53383/149‑1 (MQ=255)
                                                                                                                                               tCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCTCAAt        <  1:151799/148‑1 (MQ=255)
                                                                                                                                               tCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCTCAATg       <  8:346993/149‑1 (MQ=255)
                                                                                                                                                 cACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCTc           >  1:41682/1‑143 (MQ=255)
                                                                                                                                                 cACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCTc           <  2:41682/143‑1 (MQ=255)
                                                                                                                                                 cACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCTCAATgg      >  4:303648/1‑148 (MQ=255)
                                                                                                                                                   ccTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCt                                           <  2:105142/109‑1 (MQ=255)
                                                                                                                                                   ccTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCt                                           >  1:105142/1‑109 (MQ=255)
                                                                                                                                                    cTAAAAGTGGACACCATTTCTTGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCTCAATGGCAAc  <  6:281384/149‑1 (MQ=255)
                                                                                                                                                    cTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCTCAATGGCAAc  >  1:173140/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGTCTGCAGTCAGCAAGCAGGTATCAAAGCATTGAAAATGCCTTGTCCCGTACACAAAAAAACTGACACTCCCCTGATAGTCGAATCCCGGTATCCGTCCGGCCAACACATCTTCCTGACGAAACCGGCGAAGGCCATGGTGTCCACCTAAAAGTGGACACCATTTCTGGCCCATCAAGCGGATTTTCCAGGCATCCTCAGCGCCGTACCTACAATAAACCTTTCAAAGCCCAGAGTGCGACGAGCCCGGCGCCTCCATCGCAAACATCGCCTTGGGCCACAGCCTCAATGGCAAC  >  NC_002947/1017686‑1017982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑