Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,022,797 T→A 6.4% N306Y (AAT→TAT)  dppA‑I ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,022,7970TA 6.4% 187.1 / 2.5 62N306Y (AAT→TAT) dppA‑Idipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (30/28);  new base A (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TAGCCGATCATGGTGGGTGGGTAAGGGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCACTCGTTGGCGCGCAGTTTCTGCAGG  >  NC_002947/1022652‑1022930
                                                                                                                                                  |                                                                                                                                     
tAGCCGATCATGGTGGGTGGGTAAGGGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCa                                                                                                                                     <  2:22811/148‑1 (MQ=255)
  gCCGATCATGGTGGGTGGGTAAGGGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATg                                                                                                                                   <  8:280222/148‑1 (MQ=255)
     gATCATGGTGGGTGGGTAAGGGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGa                                                                                                                               >  3:55503/1‑149 (MQ=255)
        cATGGTGGGTGGGTAAGGGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGt                                                                                                                            >  3:42336/1‑149 (MQ=255)
            gtgggtggGTAAGGGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGcc                                                                                                                        <  1:356435/149‑1 (MQ=255)
                ggtggtAAGTGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTg                                                                                                                    <  8:328929/146‑1 (MQ=255)
                  gggtAAGGGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGAc                                                                                                                  >  7:338926/1‑149 (MQ=255)
                     tAAGGGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATg                                                                                                                                   <  2:144112/129‑1 (MQ=255)
                     tAAGGGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCg                                                                                                                                >  8:193538/1‑132 (MQ=255)
                               cacccaccAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGAtt                                                                                                     <  5:315363/149‑1 (MQ=255)
                                acccaccAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGAttt                                                                                                    >  1:7882/1‑149 (MQ=255)
                                  ccaccaGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCGAAAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTTTCATGGGTATTAATGGCGATGTAGCCGGTCACCAGGGCCTCGAtttg                                                                                                   >  1:281787/1‑148 (MQ=255)
                                  ccaccaGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATttct                                                                                                  >  7:22259/1‑149 (MQ=255)
                                  ccaccaGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATttct                                                                                                  >  3:249509/1‑149 (MQ=255)
                                  ccaccaGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATttct                                                                                                  >  5:33870/1‑149 (MQ=255)
                                  ccaccaGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATCGCTTTACGCACCCGCACGTCGTTGAGGTAATAGTGCAGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATttct                                                                                                  >  3:91999/1‑149 (MQ=255)
                                    accaGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATttctt                                                                                                 >  5:320954/1‑148 (MQ=255)
                                      caGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCaa                                                                                              >  1:155445/1‑149 (MQ=255)
                                      caGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCaa                                                                                              >  1:174193/1‑149 (MQ=255)
                                        gTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAAcc                                                                                            <  7:121015/149‑1 (MQ=255)
                                        gTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAAcc                                                                                            <  4:42336/149‑1 (MQ=255)
                                           cGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTg                                                                                         <  8:22259/149‑1 (MQ=255)
                                           cGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTg                                                                                         >  4:83409/1‑149 (MQ=255)
                                            gTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGa                                                                                        <  3:247384/149‑1 (MQ=255)
                                            gTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGa                                                                                        <  8:161026/149‑1 (MQ=255)
                                             ttGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAg                                                                                       >  6:32916/1‑149 (MQ=255)
                                             ttGCCTTTGCCAAACACCTGGTCGACATGGGTCTGGCGGTCG‑CAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAg                                                                                       <  4:91999/149‑1 (MQ=255)
                                                    tGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCgg                                                                                 <  4:55503/148‑1 (MQ=255)
                                                    tGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCg                                                                                  <  2:281787/147‑1 (MQ=255)
                                                          aCAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTcttgc                                                                          <  6:101184/149‑1 (MQ=255)
                                                                 gTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATc                                                                    >  1:215360/1‑148 (MQ=255)
                                                                 gTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATACATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATc                                                                    >  3:105444/1‑148 (MQ=255)
                                                                 gTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATACATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATc                                                                    >  7:270354/1‑148 (MQ=255)
                                                                    gACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATg                                                                <  6:320954/149‑1 (MQ=255)
                                                                       aTGGGTCTGGCGGTCG‑AAGGCCATGCTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCa                                                             >  5:38131/1‑149 (MQ=255)
                                                                         gggTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACg                                                           >  4:139581/1‑149 (MQ=255)
                                                                            tCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCa                                                        >  4:219646/1‑149 (MQ=255)
                                                                              tGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATc                                                      <  4:249509/149‑1 (MQ=255)
                                                                                  ggTCG‑AAGGCCATGTTGATGGCTTTATGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGc                                                  >  5:188549/1‑149 (MQ=255)
                                                                                     cG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTg                                               <  2:215360/149‑1 (MQ=255)
                                                                                     cG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTg                                               <  3:83409/149‑1 (MQ=255)
                                                                                      g‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTgg                                              <  2:155445/149‑1 (MQ=255)
                                                                                      g‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCCGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCaa                                                                                              >  6:64821/1‑101 (MQ=255)
                                                                                         aGGCCATGTTGATGGCTTTACTCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGa                                            <  5:32916/149‑1 (MQ=255)
                                                                                           gCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATACATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATa                                          <  4:105444/149‑1 (MQ=255)
                                                                                           gCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATACATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATa                                          <  8:270354/149‑1 (MQ=255)
                                                                                             cATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATa                                          >  4:210339/1‑147 (MQ=255)
                                                                                               tGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATagagt                                      <  3:139581/149‑2 (MQ=255)
                                                                                               tGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAgc                                      <  2:7882/149‑1 (MQ=255)
                                                                                                   gATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCa                                  >  4:182524/1‑149 (MQ=255)
                                                                                                    aTGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCAc                                 >  3:216245/1‑149 (MQ=255)
                                                                                                           tACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCa                           <  6:38131/148‑1 (MQ=255)
                                                                                                              gcacgcgcacgTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTg                                               >  6:32648/1‑125 (MQ=255)
                                                                                                              gcacgcgcacgTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTg                                               <  5:32648/125‑1 (MQ=255)
                                                                                                              gcacgcgcacgTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCACTCg                       <  6:33870/149‑1 (MQ=255)
                                                                                                                  gcgcacgTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTACAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCACTCGTTgg                   >  5:31964/1‑149 (MQ=255)
                                                                                                                    gcacgTCGGTGCGGTATTTGTGCTGGGTATTCATGGCGATGGAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCACTCGTTGgcg                 <  8:338926/149‑1 (MQ=255)
                                                                                                                      acgTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCATAGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATAGATGTAACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCACTAGTTGgcgcg               >  7:313465/1‑149 (MQ=255)
                                                                                                                        gtcgtTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCACTCGTTGgcgcg               >  1:141663/1‑147 (MQ=255)
                                                                                                                            gtGAGGGATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCACTCGTTGGCGCGCAGttt         <  2:125792/148‑1 (MQ=255)
                                                                                                                                ggTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCACTCGTTTGCGCGCAGTTTCTg      >  7:184256/1‑148 (MQ=255)
                                                                                                                                   aTTTGTGCTGGGTATTCATGGCGATGTAGCGGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCACTCGTTGGCGCGCAGTTTCTGCAgg  >  1:30919/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                     
TAGCCGATCATGGTGGGTGGGTAAGGGTTTACACCCACCAGTGCGTTGCCTTTGCCAAACAGTTGGTCGACATGGGTCTGGCGGTCG‑AAGGCCATGTTGATGGCTTTACGCACGCGCACGTCGTTGAGGTATTTGTGCTGGGTATTCATGGCGATGTAGCCGGTGACCAGGGCCTCGATTTCTTCAACCTTGAGTTTCGGGTCTTGCTTGATCGATGGCACGTCATCGGGCTTGGGATAGAGCGCCACCTGGCACTCGTTGGCGCGCAGTTTCTGCAGG  >  NC_002947/1022652‑1022930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑