Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,038,222	C→A	8.2%	P391P (CCG→CCT)	PP_0897 ←	class 1 fumarate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,038,222	0	C	A	8.2%	216.1 / 6.3	69	P391P (CCG→CCT)	PP_0897	class 1 fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base C (30/33);  new base A (4/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CCGCCAACGGCCATCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTC  >  NC_002947/1038076‑1038366                                                                                                                                                   |                                                                                                                                                   ccgccAACGGCCATCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCgg                                                                                                                                                  <  1:352988/149‑1 (MQ=255)     cAACGGCCATCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCg                                                                                                                                              >  4:10891/1‑149 (MQ=255)      aaCGGCCATCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCgg                                                                                                                                             >  3:147126/1‑149 (MQ=255)              tCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGGCCCAGCCGGcccg                                                                                                                                     >  7:345298/1‑148 (MQ=255)                aGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TAc                                                                                                                                 <  3:10891/149‑1 (MQ=255)                  gTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGGCCCAGCCGGCCC‑‑GACCa                                                                                                                               <  8:345298/149‑1 (MQ=255)                     cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACtt                                                                                                                            >  1:120666/1‑149 (MQ=255)                     cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATAAGGGTAGCAGTTGTCGGGCCGGCTGGGCC‑‑TAACACt                                                                                                                             >  6:91758/1‑148 (MQ=255)                                 gtCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTcacccacc                                                                                                                 <  3:185704/148‑1 (MQ=255)                                 gtCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATATGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCg                                                                                                                >  2:283383/1‑149 (MQ=255)                                           gCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCgg                                                                                                      >  6:205932/1‑149 (MQ=255)                                           gCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCgg                                                                                                      >  2:368604/1‑149 (MQ=255)                                            ccTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCggg                                                                                                     >  1:90764/1‑149 (MQ=255)                                                 aTGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGAc                                                                                                <  7:300336/149‑1 (MQ=255)                                                 aTGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGAc                                                                                                <  5:361445/149‑1 (MQ=255)                                                  tGGCGGTCGGGCCACTCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGTCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGAc                                                                                                <  8:331768/148‑1 (MQ=255)                                                  tGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACg                                                                                               <  1:368604/149‑1 (MQ=255)                                                     cGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACgtag                                                                                            <  4:173509/149‑1 (MQ=255)                                                      ggTCGGGCCACGCTCGGACTTGCCAATCTTGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACgtag                                                                                            >  1:207705/1‑148 (MQ=255)                                                              cACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAg                                                                                   <  2:131443/149‑1 (MQ=255)                                                              cACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAg                                                                                   >  4:99757/1‑149 (MQ=255)                                                                 gCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCgg                                                                                >  1:148387/1‑149 (MQ=255)                                                                      gACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCttt                                                                           >  8:151888/1‑149 (MQ=255)                                                                      gACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCttt                                                                           >  6:273731/1‑149 (MQ=255)                                                                         ttGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                        >  6:133015/1‑149 (MQ=255)                                                                            ccAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTc                                                                     >  4:314657/1‑149 (MQ=255)                                                                            ccAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTc                                                                     >  2:314421/1‑149 (MQ=255)                                                                              aaTCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGtagtag                                                                                         <  1:29821/127‑1 (MQ=255)                                                                              aaTCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGtagtag                                                                                         >  2:29821/1‑127 (MQ=255)                                                                               aTCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTAc                                                                  <  1:283383/149‑1 (MQ=255)                                                                                  aTGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCg                                                                <  3:314657/148‑1 (MQ=255)                                                                                      ccAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAAc                                                           >  8:231774/1‑149 (MQ=255)                                                                                       cagcagGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAAc                                                           >  5:8970/1‑148 (MQ=255)                                                                                        agcagGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAActc                                                         <  7:151888/149‑1 (MQ=255)                                                                                        agcagGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAActc                                                         <  5:273731/149‑1 (MQ=255)                                                                                           agGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTc                                                      <  3:99757/149‑1 (MQ=255)                                                                                           agGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTc                                                      <  2:120666/149‑1 (MQ=255)                                                                                            ggCCAGTCTGCTCAAGGAGCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCg                                                     <  6:251291/149‑1 (MQ=255)                                                                                             gCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGc                                                                                  <  3:183064/119‑1 (MQ=255)                                                                                             gCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGc                                                                                  >  4:183064/1‑119 (MQ=255)                                                                                                  tCTGCTCAAGGATCTGGCGAGTGAACTTGTCGATACGGGTAGCAGTTTTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCTGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACgg                                               <  2:90764/149‑1 (MQ=255)                                                                                                  tCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACgg                                               <  3:78594/149‑1 (MQ=255)                                                                                                      cTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTc                                                      <  8:3711/138‑1 (MQ=255)                                                                                                      cTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTc                                                      >  7:3711/1‑138 (MQ=255)                                                                                                      cTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGtt                                             <  5:91758/147‑1 (MQ=255)                                                                                                      cTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGa                                           <  3:126511/149‑1 (MQ=255)                                                                                                      cTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGa                                           <  4:356072/149‑1 (MQ=255)                                                                                                       tCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCGATACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGtt                                             >  1:290109/1‑148 (MQ=255)                                                                                                       tCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAg                                          >  5:338952/1‑149 (MQ=255)                                                                                                       tCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAg                                          >  1:20502/1‑149 (MQ=255)                                                                                                       tCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAg                                          >  4:170094/1‑149 (MQ=255)                                                                                                               cTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCa                                   <  1:314421/148‑1 (MQ=255)                                                                                                                  gCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCgg                                                                                <  6:216364/100‑1 (MQ=255)                                                                                                                  gCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCgg                                                                                >  5:216364/1‑100 (MQ=255)                                                                                                                  gCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTc                                                                          <  6:282949/106‑1 (MQ=255)                                                                                                                  gCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCaa                                  <  5:205932/146‑1 (MQ=255)                                                                                                                  gCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCa                               >  6:354366/1‑149 (MQ=255)                                                                                                                    gAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCttt                                                                           <  2:114493/103‑1 (MQ=255)                                                                                                                    gAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCAGGCCGACGTAGTAGATGAAGCGGCCttt                                                                           >  1:114493/1‑103 (MQ=255)                                                                                                                       tGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATgcgc                          >  8:57842/1‑149 (MQ=255)                                                                                                                          aCTTGTCCATACGGGTAGCAGTGGGCGGGCCGGCGGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTtg                       <  5:302890/149‑1 (MQ=255)                                                                                                                                     cGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATc            <  6:338952/149‑1 (MQ=255)                                                                                                                                         acgaaGTGGTAGGCCCAGCCGGCCC‑‑GACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTgcgc                  >  6:99245/5‑139 (MQ=255)                                                                                                                                         acgaaGTGGTAGGCCCAGCCGGCCC‑‑GACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTgcgc                  <  5:99245/135‑1 (MQ=255)                                                                                                                                         acgaaGTGGTAGGCCCAGCCGGCCC‑‑GACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACAATGCGATTGTGCTCGGCGTCACgg        >  6:256416/5‑149 (MQ=255)                                                                                                                                         acgaaGTGGTAGGCCCAGCCGGCCC‑‑GACACCTTAGTCACCCACAGGGTCGACCGGGCCGACGTAGTAGATGTCGCGGGCTTTCAGGTCTACCGGGCAATCTTCGCAACGGTTGAGAATTTCACAAATACGAATGTGCGAGTCATCACg         >  6:256418/5‑148 (MQ=255)                                                                                                                                            cAGTGGTCGGGCCGGCTGGGCC‑‑TACCCATTCGTAACACACCGTGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCg     >  7:37928/1‑149 (MQ=255)                                                                                                                                            cAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCg     <  2:20502/149‑1 (MQ=255)                                                                                                                                               tGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTc  <  4:147126/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CCGCCAACGGCCATCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCC‑‑TACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTC  >  NC_002947/1038076‑1038366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑