Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,039,146 G→T 5.4% R83R (CGC→CGA)  PP_0897 ← class 1 fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,039,1460GT 5.4% 238.3 / 3.1 74R83R (CGC→CGA) PP_0897class 1 fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (34/36);  new base T (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

ACTGCCGGGGTGTTGTCCTTGGTGTTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATA  >  NC_002947/1039000‑1039289
                                                                                                                                                  |                                                                                                                                               
aCTGCCGGGGTGTTGGCCTTGGTGTTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCGgcg                                                                                                                                               >  3:49973/1‑149 (MQ=255)
 cTGCCGGGGTGTTGTCCTTGGTGTTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAgcgc                                                                                                                                              >  2:78761/1‑149 (MQ=255)
    ccGGGGTGTTGTCCTTGGTGTTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACg                                                                                                                                           >  8:130813/1‑149 (MQ=255)
                 cTTGGTGTTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCAcgc                                                                                                                              >  2:40138/1‑149 (MQ=255)
                 cTTGGTGTTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCAcgc                                                                                                                              >  4:205484/1‑149 (MQ=255)
                    ggTGTTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGa                                                                                                                           >  7:279313/1‑149 (MQ=255)
                     gtgtTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCGGCGCACGTCCATGCCCACGCGGAt                                                                                                                          <  4:49973/149‑1 (MQ=255)
                     gtgtTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGAt                                                                                                                          <  8:279313/149‑1 (MQ=255)
                     gtgtTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGAt                                                                                                                          <  2:39707/149‑1 (MQ=255)
                           ttGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCACGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAacac                                                                                                                    >  2:202587/1‑149 (MQ=255)
                             gCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAacac                                                                                                                    <  5:137013/147‑1 (MQ=255)
                               gggCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCAAGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTg                                                                                                                >  7:348751/1‑149 (MQ=255)
                                ggCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGa                                                                                                               <  1:78761/149‑1 (MQ=255)
                                ggCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGa                                                                                                               <  8:337037/149‑1 (MQ=255)
                                  cACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGAc                                                                                                              >  6:1218/1‑148 (MQ=255)
                                      ggccggGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATAc                                                                                                         >  4:311473/1‑149 (MQ=255)
                                             tCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTc                                                                                                  <  5:1218/149‑1 (MQ=255)
                                                gCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTg                                                                                               >  7:59667/1‑149 (MQ=255)
                                                gCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACCATACCGGTGTCCTg                                                                                               >  3:288407/1‑149 (MQ=255)
                                                  cAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGc                                                                                             <  5:63867/149‑1 (MQ=255)
                                                   aGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCa                                                                                            <  8:212530/149‑1 (MQ=255)
                                                      aTCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGAt                                                                                         >  3:69924/1‑149 (MQ=255)
                                                         gAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTg                                                                                       <  7:130813/148‑1 (MQ=255)
                                                           aGCGCGCAGGACGTTTTAAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACAGGTGTCCCGGCAGATTGGAc                                                                                    >  6:25853/1‑149 (MQ=255)
                                                             cgcgcAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGa                                                                                  <  1:202587/149‑1 (MQ=255)
                                                                       gTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTgg                                                                        <  1:40138/149‑1 (MQ=255)
                                                                       gTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTgg                                                                        >  3:53854/1‑149 (MQ=255)
                                                                         tttCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGgcg                                                                      >  6:364689/1‑149 (MQ=255)
                                                                           tCAGGCAGGTTGTAGGCGCGTCGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGgcgc                                                                     <  8:166036/148‑1 (MQ=255)
                                                                             aggcaggTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCCGGCAGATTGGACGATGGCCGGTGGCGcac                                                                   <  5:25853/148‑1 (MQ=255)
                                                                                    ttGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGa                                                           <  3:54094/149‑1 (MQ=255)
                                                                                         ggCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGt                                                         <  5:137956/146‑1 (MQ=255)
                                                                                         ggCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGt                                                         >  6:137956/1‑146 (MQ=255)
                                                                                              gACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAg                                                  >  3:68655/1‑148 (MQ=255)
                                                                                                cGCACACCTTCTGTGATCAGGTCGTCGACGCTCAGGGTGGCGCCATCCCAGCGCACGGCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGAt                                               <  8:348751/149‑1 (MQ=255)
                                                                                                     acgttagTTGATCATGTCGTCGACGCTCATGGTCGCGACATCCCAGCTCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGTCGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTggg                                          <  3:205275/143‑1 (MQ=255)
                                                                                                         tCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGAt                                      >  2:162555/1‑149 (MQ=255)
                                                                                                              gATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAAt                                 >  8:327881/1‑149 (MQ=255)
                                                                                                                tCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATcg                               >  4:136909/1‑149 (MQ=255)
                                                                                                                  aTGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATcgc                              <  1:162555/148‑1 (MQ=255)
                                                                                                                   tGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGt                            <  3:205484/149‑1 (MQ=255)
                                                                                                                    gtcgtcGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTg                           >  5:56288/1‑149 (MQ=255)
                                                                                                                    gtcgtcGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTg                           >  7:121007/1‑149 (MQ=255)
                                                                                                                     tcgtcgACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTgg                                                                        >  8:340849/1‑103 (MQ=255)
                                                                                                                     tcgtcgACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTgg                                                                        <  7:340849/103‑1 (MQ=255)
                                                                                                                     tcgtcgACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGAt                                      <  4:10595/137‑1 (MQ=255)
                                                                                                                     tcgtcgACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGAt                                      >  3:10595/1‑137 (MQ=255)
                                                                                                                     tcgtcgACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTg                           <  3:116270/148‑1 (MQ=255)
                                                                                                                      cgtcgACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTg                           >  1:52232/1‑147 (MQ=255)
                                                                                                                       gtcgACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTgca                         <  3:9519/148‑1 (MQ=255)
                                                                                                                       gtcgACGCTCATGGT‑GCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGGgcagc                       >  4:224572/1‑149 (MQ=255)
                                                                                                                           aCGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAgg                    <  8:59667/149‑1 (MQ=255)
                                                                                                                             gCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGaa                                  >  1:347162/1‑133 (MQ=255)
                                                                                                                             gCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGaa                                  <  2:347162/133‑1 (MQ=255)
                                                                                                                             gCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGc                   >  1:334774/1‑148 (MQ=255)
                                                                                                                              cTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGa                 <  4:53854/149‑1 (MQ=255)
                                                                                                                               tCATGGTGGCGCCATCCCATCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGc                                                                                             >  1:189580/1‑72 (MQ=255)
                                                                                                                               tCATGGTGGCGCCATCCCATCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGc                                                                                             >  1:195496/1‑72 (MQ=255)
                                                                                                                               tCATGGTGGCGCCATCCCATCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGc                                                                                             <  2:189580/72‑1 (MQ=255)
                                                                                                                               tCATGGTGGCGCCATCCCATCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGc                                                                                             <  2:195496/72‑1 (MQ=255)
                                                                                                                               tCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGAt                >  7:156466/1‑149 (MQ=255)
                                                                                                                                  tggtggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCCTGCTGCACGCGATTcc             <  7:327881/149‑1 (MQ=255)
                                                                                                                                   ggtggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGGACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCt            >  6:14851/1‑149 (MQ=255)
                                                                                                                                   ggtggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCt            >  1:320116/1‑149 (MQ=255)
                                                                                                                                    gtggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTc           <  3:311473/149‑1 (MQ=255)
                                                                                                                                     tggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCa          <  5:364689/149‑1 (MQ=255)
                                                                                                                                     tggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCa          >  2:353861/1‑149 (MQ=255)
                                                                                                                                     tggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCa          <  6:22425/149‑1 (MQ=255)
                                                                                                                                        cgcCATCCCAGCGCACGTCCATGCCGATGGGGATGAACTCGGGGTGGTTACGGGTGTGCTGGCAGATGGGACGATTGCAGGTGGCGCAGATGCTCGAGTTGATCTGGATCTGGGCGTTGGATTCGCGTGCAGCAGGCGATTCCTCAGGc       <  4:288407/149‑1 (MQ=255)
                                                                                                                                         gcCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGgc                                                                       >  7:54137/1‑84 (MQ=255)
                                                                                                                                         gcCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGgc                                                                       <  8:54137/84‑1 (MQ=255)
                                                                                                                                         gcCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGt                            <  8:123742/127‑1 (MQ=255)
                                                                                                                                         gcCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGt                            >  7:123742/1‑127 (MQ=255)
                                                                                                                                           cATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACg        >  3:321512/1‑145 (MQ=255)
                                                                                                                                            aTCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGAt   <  2:320116/149‑1 (MQ=255)
                                                                                                                                             tCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATa  <  3:136909/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACTGCCGGGGTGTTGTCCTTGGTGTTCTTGCGGGCACCGGCCGGGTCGGCCAGGATCGAAGCGCGCAGGACGTTTTCAGGCAGGTTGTAGGCGCGACGCACACCTTCGTTGATCATGTCGTCGACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATA  >  NC_002947/1039000‑1039289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑