Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,053,653 C→G 5.9% W70C (TGG→TGC)  PP_0911 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,053,6530CG 5.9% 208.7 / 3.4 68W70C (TGG→TGC) PP_0911hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/34);  new base G (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

GACCAGGTTGACGATCAGGTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGTGCTGCGCGCCGACTGCCAGGCTGTTGCTGCGGTTTTCACCGTCGA  >  NC_002947/1053506‑1053801
                                                                                                                                                   |                                                                                                                                                    
gACCAGGTTGACGATCAGGTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAAc                                                                                                                                                      <  5:371009/148‑1 (MQ=255)
gACCAGGTTGACGATCAGGTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAAc                                                                                                                                                      <  8:116205/148‑1 (MQ=255)
     ggTTGACGATCAGGTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATg                                                                                                                                                >  2:249310/1‑149 (MQ=255)
       ttGACGATCAGGTTCTTGTTGCCGCCCCCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGtt                                                                                                                                              >  2:358731/1‑149 (MQ=255)
         gACGATCAGGTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTgg                                                                                                                                            >  2:113168/1‑149 (MQ=255)
         gACGATCAGGTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTg                                                                                                                                             <  8:68217/148‑1 (MQ=255)
         gACGATCAGGTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTg                                                                                                                                             <  1:264903/148‑1 (MQ=255)
            gATTAGGTTCTTGTTGCAGCCAACGCTATTATCGCCTGTGACTTCGATGACTTCTTCGCTTGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGgtgt                                                                                                                                         <  6:256511/149‑1 (MQ=255)
                aGGTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCgg                                                                                                                                      <  3:1820/148‑1 (MQ=255)
                  gTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAAGCAGATGTTGGTGTCgg                                                                                                                                      >  8:195077/1‑146 (MQ=255)
                  gTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAAGCAGATGTTGGTGTCgg                                                                                                                                      >  8:189815/1‑146 (MQ=255)
                  gTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCgg                                                                                                                                      >  5:249379/1‑146 (MQ=255)
                            ccgccACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGa                                                                                                                          <  7:324065/148‑1 (MQ=255)
                            ccgccACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAg                                                                                                                         <  8:321359/149‑1 (MQ=255)
                             gtcaccggcgATAATCG‑CGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGa                                                                                                                       >  3:42795/8‑149 (MQ=255)
                              gccACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTTGGCAGACGGGCCGAGGa                                                                                                                       <  1:355634/149‑1 (MQ=255)
                               ccACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGAt                                                                                                                      <  1:113168/149‑1 (MQ=255)
                                  ccGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAg                                                                                                                   >  2:152834/1‑149 (MQ=255)
                                  ccGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAg                                                                                                                   <  1:249310/149‑1 (MQ=255)
                                   cgcgATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAgg                                                                                                                  <  3:12074/149‑1 (MQ=255)
                                   cgcgATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAgg                                                                                                                  >  7:265684/1‑149 (MQ=255)
                                   cgcgATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAgg                                                                                                                  >  3:76380/1‑149 (MQ=255)
                                   cgcgATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAgg                                                                                                                  >  2:21181/1‑149 (MQ=255)
                                    gcgATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGgc                                                                                                                 >  4:319225/1‑149 (MQ=255)
                                      gATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGgcg                                                                                                                >  2:64990/1‑148 (MQ=255)
                                      gATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGgcg                                                                                                                >  6:376682/1‑148 (MQ=255)
                                           tCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGc                                                                                                          <  7:238940/149‑1 (MQ=255)
                                           tCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGc                                                                                                          >  5:349311/1‑149 (MQ=255)
                                             gCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAAGCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGcc                                                                                                         <  7:195077/148‑1 (MQ=255)
                                             gCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAAGCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGcc                                                                                                         <  7:189815/148‑1 (MQ=255)
                                                 gTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTtgtg                                                                                                    <  1:358731/149‑1 (MQ=255)
                                                  tGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCGTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTgg                                                                                                   >  4:285482/1‑149 (MQ=255)
                                                      ttCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTtct                                                                                               >  5:375493/1‑149 (MQ=255)
                                                      ttCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTtc                                                                                                >  8:302866/1‑148 (MQ=255)
                                                        cGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCTGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTtct                                                                                               <  2:165936/147‑1 (MQ=255)
                                                        cGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTtct                                                                                               <  6:249379/147‑1 (MQ=255)
                                                        cGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTtct                                                                                               <  8:265684/147‑1 (MQ=255)
                                                        cGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTtct                                                                                               <  4:76380/147‑1 (MQ=255)
                                                         gATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCa                                                                                             <  6:375493/148‑1 (MQ=255)
                                                               ttcttcGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTtct                                                                                               >  1:424/1‑140 (MQ=255)
                                                               ttcttcGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTtct                                                                                               <  2:424/140‑1 (MQ=255)
                                                                    cGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGc                                                                                   <  6:349311/147‑1 (MQ=255)
                                                                     gCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGa                                                                                >  3:57252/1‑149 (MQ=255)
                                                                         gtgtATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATcc                                                                             >  8:243526/1‑148 (MQ=255)
                                                                         gtgtATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCa                                                                            >  5:212945/1‑149 (MQ=255)
                                                                             aTTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACgg                                                                         <  7:243526/148‑1 (MQ=255)
                                                                              ttCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACggg                                                                        <  1:152834/148‑1 (MQ=255)
                                                                                ccATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGAt                                                                      <  7:222474/148‑1 (MQ=255)
                                                                                ccATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATg                                                                     >  6:62976/1‑149 (MQ=255)
                                                                                 cATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATgg                                                                    >  6:334473/1‑149 (MQ=255)
                                                                                 cATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATg                                                                     <  4:178291/148‑1 (MQ=255)
                                                                                 cATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATg                                                                     >  3:178291/1‑148 (MQ=255)
                                                                                  aTGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGc                                                                   <  4:57252/149‑1 (MQ=255)
                                                                                     gaggagGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTTTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCa                                                                 <  1:64990/148‑1 (MQ=255)
                                                                                         aggCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAg                                                            <  7:302866/149‑1 (MQ=255)
                                                                                         aggCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAg                                                            <  5:324176/149‑1 (MQ=255)
                                                                                         aggCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAg                                                            <  4:42795/149‑1 (MQ=255)
                                                                                          ggCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGc                                                           <  1:21181/149‑1 (MQ=255)
                                                                                              gTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCAtt                                                       <  3:345974/149‑1 (MQ=255)
                                                                                                 aTGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTc                                                      >  2:303223/1‑147 (MQ=255)
                                                                                                     aCTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACg                                                >  6:173906/1‑149 (MQ=255)
                                                                                                      cTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGt                                               <  3:319225/149‑1 (MQ=255)
                                                                                                                tCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGTGCTGCGCGcc                                     <  1:303223/149‑1 (MQ=255)
                                                                                                                   aTGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGTGCTGCGCGCCGAc                                  >  7:117886/1‑149 (MQ=255)
                                                                                                                       gcgcAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGATCCATTCCAGACGTGCTGCGCGCCGACTGc                               >  8:175258/1‑148 (MQ=255)
                                                                                                                        cgcAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGTGCTGCGCGCCGACTGcc                              <  5:376682/148‑1 (MQ=255)
                                                                                                                                   aGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGTGCTGCGCGCCGACTGCCAGGCTGTtgctg                  >  1:310535/1‑149 (MQ=255)
                                                                                                                                    gTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGTGCTGCGCGCCGACTGCCAGGCTGTtgctgc                 >  3:1385/1‑149 (MQ=255)
                                                                                                                                          cTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGTGCTGCGCGCCGACTGCCAGGCTGTTGCTGCGGtttt           <  4:1385/149‑1 (MQ=255)
                                                                                                                                          cTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGTGCTGCGCGCCGACTGCCAGGCTGTTGCTGCGGtttt           >  5:112793/1‑149 (MQ=255)
                                                                                                                                                   ccAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGTGCTGCGCGCCGACTGCCAGGCTGTTGCTGCGGTTTTCACCGTCGa  >  6:6529/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GACCAGGTTGACGATCAGGTTCTTGTTGCCGCCACCGCGATTATCGCCGGTGACTTCGATGACTTCTTCGCTGGTGTATTCCATGGAGGAGGCGGTAATGCACTTGACCGCGTCCATGTGCGCAGGTTGCCAGTAATCCTGGAATAACCAGATGTTGGTGTCGGCAGACGGGCCGAGGATCAGGCGCAAGGCCTTGTGGTTCTCAGTCGATCTGCCGATCCACGGGATGGCCAGCGAGCCATTCCAGACGTGCTGCGCGCCGACTGCCAGGCTGTTGCTGCGGTTTTCACCGTCGA  >  NC_002947/1053506‑1053801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑