Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,067,651 G→A 9.7% intergenic (+4/‑184) PP_0925 → / → PP_0926 hypothetical protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,067,6510GA 9.7% 109.8 / 2.8 41intergenic (+4/‑184)PP_0925/PP_0926hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (19/18);  new base A (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

GGGGAGATCGATGCGGCGTTGGCGACAGGGCCGGTGCAGTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGACATTTCCTTTTATCGCGCTTGATCCCCGCAAATCCCAGCCGTTGACGTAT  >  NC_002947/1067507‑1067799
                                                                                                                                                |                                                                                                                                                    
ggggAGATCGATGCGGCGTTGGCGACAGGGCCGGTGCAGTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTca                                                                                                                                                  <  7:296617/149‑1 (MQ=255)
  ggAGATCGATGCGGCGTTGGCGACAGGGCCGGTGCAGTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTca                                                                                                                                                  >  8:317074/1‑147 (MQ=255)
       tCGATGCGGCGTTGGCGACAGGGCCGGTGCAGTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGttt                                                                                                                                           <  6:333999/149‑1 (MQ=255)
           tGCGGCGTTGGCGACAGGGCCGGTGCAGTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTtgtgt                                                                                                                                       <  2:254152/149‑1 (MQ=255)
                  ttGGCGACAGGGCCGGTGCAGTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGACTcaca                                                                                                                                                >  6:51482/1‑133 (MQ=255)
                  ttGGCGACAGGGCCGGTGCAGTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGACTcaca                                                                                                                                                <  5:51482/133‑1 (MQ=255)
                   tGGCGACAGGGCCGGTGCAGTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGAGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGAt                                                                                                                               >  4:265084/1‑149 (MQ=255)
                                    cAGTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTAc                                                                                                              <  3:265084/149‑1 (MQ=255)
                                      gTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTcaca                                                                                                                                                <  7:157118/113‑1 (MQ=255)
                                      gTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTcaca                                                                                                                                                >  8:157118/1‑113 (MQ=255)
                                      gTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGttt                                                                                                                                           >  1:220152/1‑118 (MQ=255)
                                      gTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGttt                                                                                                                                           <  2:220152/118‑1 (MQ=255)
                                          gcgcAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTgg                                                                                                        >  5:90613/1‑149 (MQ=255)
                                            gcAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTgggg                                                                                                      >  4:142549/1‑149 (MQ=255)
                                                 tCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCggg                                                                                                 >  2:185644/1‑149 (MQ=255)
                                                 tCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCggg                                                                                                 <  2:304396/149‑1 (MQ=255)
                                                   tGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGt                                                                                               >  5:114669/1‑149 (MQ=255)
                                                      cAGCGTGACCATGGGGTGATGGCGCTGGCGGCGGCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAg                                                                                            <  6:1319/149‑1 (MQ=255)
                                                         cGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTg                                                                                         >  4:78022/1‑149 (MQ=255)
                                                           tGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCa                                                                                       <  6:8494/149‑1 (MQ=255)
                                                            gACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCa                                                                                       >  6:149305/1‑148 (MQ=255)
                                                               cAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCTTGAATGGCTCACAGGTTTGTGTGAACCGAt                                                                                                                               >  1:218744/1‑105 (MQ=255)
                                                               cAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCTTGAATGGCTCACAGGTTTGTGTGAACCGAt                                                                                                                               <  2:218744/105‑1 (MQ=255)
                                                               cAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGc                                                                                   >  2:319845/1‑149 (MQ=255)
                                                                     gTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCgg                                                                             <  6:114669/149‑1 (MQ=255)
                                                                       gATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGACTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTa                                                                           >  5:30117/1‑149 (MQ=255)
                                                                         tGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACt                                                                         >  4:169805/1‑149 (MQ=255)
                                                                                   ggcgTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGttt                                                                                                                                           <  1:205625/73‑1 (MQ=255)
                                                                                   ggcgTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGttt                                                                                                                                           >  2:205625/1‑73 (MQ=255)
                                                                                      gTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGACTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAAc                                                            <  6:30117/149‑1 (MQ=255)
                                                                                        cGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCt                                                          <  2:369018/149‑1 (MQ=255)
                                                                                            gcgGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGaaa                                                      <  3:169805/149‑1 (MQ=255)
                                                                                               ggAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGAc                                                   <  1:185644/149‑1 (MQ=255)
                                                                                                      cGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGACATTTCCt                                            <  3:117464/149‑1 (MQ=255)
                                                                                                                  gCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGACATTTCCTTTTATCGCGCtt                                >  8:90281/1‑149 (MQ=255)
                                                                                                                    ttGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGACATTTCCTTTTATCGCGCTTGa                              >  8:328222/1‑149 (MQ=255)
                                                                                                                     tGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGACATTTCCTTTTATCGCGCTTCAt                             >  4:324318/1‑149 (MQ=255)
                                                                                                                        gctgctGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGACATTTCCTTTTATCGCGCTTGATccc                          <  3:78022/149‑1 (MQ=255)
                                                                                                                            ctgcGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGACATTTCCTTTTATCGCGCTTGATCCCCGc                       >  4:22854/1‑148 (MQ=255)
                                                                                                                                gggCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGACATTTCCTTTTATCGCGCTTGATCCCCGCAAATc                  <  7:317074/149‑1 (MQ=255)
                                                                                                                                            aaTGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGAAGAAAACCTGAAAGACATTTCCTTTTATCGCGCTTGATCCCCGCAAATCCCAGCCGTTGAc      >  3:13553/1‑149 (MQ=255)
                                                                                                                                                gCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGACATTTCCTTTTATCGCGCTTGATCCCCGCAAATCCCAGCCGTTGACGTAt  <  7:90281/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGGGAGATCGATGCGGCGTTGGCGACAGGGCCGGTGCAGTGGGCGCAAGTCTGGCAGCGTGACCAAGGGGTGATGGCGCTGGCGGCGTCGGCGCGGGAGAAGCGGCTGAACAAGGCTTGGGCTGCTGCGGGCTTTTCATGAATGGCTCACAGGTTTGTGTGAACCGATGAATCGCGGCGTGGTACCTGTGGGGGCGGGGTTAGGTGCAAAGCGGCCGGTACTGGCAGCAGAAAACCTGAAAGACATTTCCTTTTATCGCGCTTGATCCCCGCAAATCCCAGCCGTTGACGTAT  >  NC_002947/1067507‑1067799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑