Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,100,207 G→C 6.9% V132L (GTG→CTG) ‡ mlaF → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,100,2070GC 6.9% 181.6 / 4.0 58V132L (GTG→CTG) ‡mlaFABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (32/22);  new base C (2/2);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CGCCCGAAAGCAGATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGATCGCCATGG  >  NC_002947/1100059‑1100354
                                                                                                                                                    |                                                                                                                                                   
cGCCCGAAAGCAGATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCg                                                                                                                                                     >  7:312990/1‑149 (MQ=255)
    cGAAAGCAGATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTggg                                                                                                                                                 >  4:289948/1‑149 (MQ=255)
     gAAAGCAGATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGc                                                                                                                                                >  4:165678/1‑149 (MQ=255)
          cAGATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCCACGGCCTgca                                                                                                                                           <  5:30337/149‑2 (MQ=255)
             aTGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCgg                                                                                                                                        <  4:145762/149‑1 (MQ=255)
             aTGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCgg                                                                                                                                        <  4:151291/149‑1 (MQ=255)
              tGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGt                                                                                                                                       <  4:12255/149‑1 (MQ=255)
                         ttCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCt                                                                                                                            >  4:241515/1‑149 (MQ=255)
                                   gcgcGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCcgac                                                                                                                  <  3:51098/149‑1 (MQ=255)
                                      cgTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAg                                                                                                               <  5:312923/149‑1 (MQ=255)
                                              aCCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCgg                                                                                                       >  7:118449/1‑149 (MQ=255)
                                               ccGACCTCGATGTGTTCTTGAACGTCGCGTTGCCGTTGCGCGTGCATACTGAGCTGTCGGAGGAAATGATCCTTGACATCGTGTTGATGAAGCTGATGGCCGTGTGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCgtt                                                                                                      <  8:266598/149‑3 (MQ=255)
                                               ccGACCTCGATGTGTTCGAGCACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGAGATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACATGATGCCCGCCGCGCTGACCGGt                                                                                                      >  4:125467/1‑149 (MQ=255)
                                               ccGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGt                                                                                                      <  3:241515/149‑1 (MQ=255)
                                                cGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTg                                                                                                     <  3:165678/149‑1 (MQ=255)
                                                          gtgtTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAgcg                                                                                           >  6:233797/1‑149 (MQ=255)
                                                          gtgtTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAgcg                                                                                           >  6:228613/1‑149 (MQ=255)
                                                             ttCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGAGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCg                                                                                        >  3:212339/1‑149 (MQ=255)
                                                                 agaACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGccacacg                                                                                    >  8:313801/1‑144 (MQ=255)
                                                                  gaACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTgg                                                                                   >  7:44261/1‑149 (MQ=255)
                                                                      gTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCt                                                                               >  7:320239/1‑149 (MQ=255)
                                                                      gTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCt                                                                               >  2:210484/1‑149 (MQ=255)
                                                                       tCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTg                                                                              >  5:144955/1‑149 (MQ=255)
                                                                         gcgTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCcgacg                                                                                                                 >  1:97959/1‑112 (MQ=255)
                                                                         gcgTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCcgacg                                                                                                                 <  2:97959/112‑1 (MQ=255)
                                                                               ccGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTCACATCGTGATGATGAAGCTGCAGGCCGAGGGCCTGCGCGGTGCCATCGACCAGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCCTGTGGCGCTGGCCCCGGc                                                                      >  7:149107/1‑149 (MQ=255)
                                                                                        gTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCt                                                             <  3:289948/149‑1 (MQ=255)
                                                                                           cacaCTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGCGCTGTCCGGTGGCATGAAGCGCCGGGTGGCGCTGGCCCGGGCGATTGCCCTGGa                                                          >  4:109102/4‑149 (MQ=255)
                                                                                                tCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCg                                                      >  5:127437/1‑148 (MQ=255)
                                                                                                tCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCg                                                      >  7:342175/1‑148 (MQ=255)
                                                                                                tCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGAACCg                                                      >  2:106901/1‑148 (MQ=255)
                                                                                                 cAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCAAGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCg                                                      >  2:289263/1‑147 (MQ=255)
                                                                                                  aGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGc                                                     <  8:351618/147‑1 (MQ=255)
                                                                                                  aGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGc                                                     <  8:44261/147‑1 (MQ=255)
                                                                                                        cGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGGAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTc                                             <  6:160237/149‑1 (MQ=255)
                                                                                                           aCGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATg                                          >  1:366186/1‑149 (MQ=255)
                                                                                                             gAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTa                                        >  8:165332/1‑149 (MQ=255)
                                                                                                                   aTCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTacgacg                                   >  1:31799/1‑148 (MQ=255)
                                                                                                                    tCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGACCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGaa                                 >  6:209118/1‑149 (MQ=255)
                                                                                                                     ccGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAAc                                >  1:319950/1‑149 (MQ=255)
                                                                                                                          aCATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCGGCAGATCCTCATGTACGACGAACCGTtc                           <  1:210484/149‑1 (MQ=255)
                                                                                                                          aCATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTtc                           <  2:47149/149‑1 (MQ=255)
                                                                                                                           cATCGTGTTGATGATGCTGCAGGGCGTGGGCCTGCGCGGTGCCATCGACGTGTTGCCCGACGAGCTGTCCGGTGGGATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTtc                           <  4:212339/148‑1 (MQ=255)
                                                                                                                               gtgtTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCgg                      >  2:249737/1‑149 (MQ=255)
                                                                                                                                tgtTGATTCAGGGGCTGGCCGGTGTCCGGCGCGGTGCCATCGACCTGATGCGCGACGAGCTGTCCGGTGGCTTGATGCGCCGTGTGGGTGTGGCCCGTGCGTTTGCCCTGGACCCGCAGATCCTCATGTACGAGGTACCGTTCGTCTGc                     <  8:149107/149‑1 (MQ=255)
                                                                                                                                    gatgaAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAgg                 >  8:344804/1‑149 (MQ=255)
                                                                                                                                    gatgaAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAg                  >  3:5646/1‑148 (MQ=255)
                                                                                                                                     atgaAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAggc                >  4:218025/1‑148 (MQ=255)
                                                                                                                                       gaAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGAcc              >  2:252418/1‑149 (MQ=255)
                                                                                                                                        aaGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGAccc             <  1:249737/149‑1 (MQ=255)
                                                                                                                                         ccgcgCAGGCCCACGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGCCCCGCGCATCCGCATGTACGACGAACCGTTCGACGGCCAGGcccc             >  4:193504/5‑148 (MQ=255)
                                                                                                                                          gCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGa           <  8:342175/149‑1 (MQ=255)
                                                                                                                                           cTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGAt          >  3:45385/1‑149 (MQ=255)
                                                                                                                                            tGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGGAGATCCTCATGTACGACGAAACGATCGTCGGCCAGGACCCGATc         >  8:262982/1‑149 (MQ=255)
                                                                                                                                            cgCAGGCCCACGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCa                   <  3:273437/138‑1 (MQ=255)
                                                                                                                                            cgCAGGCCCACGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCa                   >  4:273437/2‑139 (MQ=255)
                                                                                                                                                   cGTGGGCCTGCGCTGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGATCGCCATgg  <  7:262982/149‑1 (MQ=255)
                                                                                                                                                   cGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCTACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCGAGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGATCGCCATgg  <  1:106901/149‑1 (MQ=255)
                                                                                                                                                   cGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGATCGCCATgg  <  8:312990/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCCGAAAGCAGATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGATCGCCATGG  >  NC_002947/1100059‑1100354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑