Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,134,089	A→T	10.2%	intergenic (+34/+44)	PP_0995 → / ← gltS	membrane protein/glutamate:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,134,089	0	A	T	10.2%	166.7 / 8.4	66	intergenic (+34/+44)	PP_0995/gltS	membrane protein/glutamate:sodium symporter
Reads supporting (aligned to +/- strand):  ref base A (31/28);  new base T (2/5);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

CCTGCATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGAAGGCGAG  >  NC_002947/1133944‑1134237                                                                                                                                                  |                                                                                                                                                      cctgcAGTGCCCGGAACTGGGCGGGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCGCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAacgacg                                                                                                                                                     <  8:201351/148‑1 (MQ=255)  ctgcATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGtt                                                                                                                                                   >  3:82669/1‑149 (MQ=255)    gcATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGTCGTTCt                                                                                                                                                 <  4:167773/149‑1 (MQ=255)     cATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTc                                                                                                                                                  <  4:146538/147‑1 (MQ=255)     cATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTc                                                                                                                                                  <  7:45252/147‑1 (MQ=255)      aTTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAacgac                                                                                                                                                      >  3:218101/1‑142 (MQ=255)      aTTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAacgac                                                                                                                                                      <  4:218101/142‑1 (MQ=255)      aTTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGa                                                                                                                                               <  3:365806/149‑1 (MQ=255)       ttGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGTCGTTCTGag                                                                                                                                              <  3:97957/149‑2 (MQ=255)          cccGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGTCGTTCTGagtt                                                                                                                                            <  1:198379/148‑4 (MQ=255)            cGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑ttgctt                                                                                                                                        <  4:263495/149‑1 (MQ=255)                     gCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCt                                                                                                                               >  1:58486/1‑149 (MQ=255)                     gCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGGTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTATGA‑TTTCTTGCCATGGCt                                                                                                                               >  8:248203/1‑149 (MQ=255)                           cGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGCCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAg                                                                                                                         >  3:275941/1‑149 (MQ=255)                                tGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCg                                                                                                                    >  2:369294/1‑149 (MQ=255)                                 cggctctgtgGTATGTCCTCGGCATGCTCGTCCCGACGGTACTCGGTCCCTGGTCGGGCCCCTGCATCCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGAAACTCAGAACGACGTACTGA‑TTGCTTGCACTCGCATCACACGTTCGt                                                                                                                   >  4:173714/7‑149 (MQ=255)                                    gtgtgtGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTc                                                                                                                     <  8:247792/144‑1 (MQ=255)                                    gtgtgtGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTc                                                                                                                     >  7:247792/1‑144 (MQ=255)                                    gtgtggGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCTGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGAc                                                                                                                <  3:173714/149‑1 (MQ=255)                                              tGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAAc                                                                                                      >  1:197943/1‑149 (MQ=255)                                                tCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAAccc                                                                                                    >  2:325562/1‑149 (MQ=255)                                                  cTCGGCATGCTCGTGCCGACGGTCCTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTCGGCTGTACCGGCAGGCCCCACGCAACTCAGACCGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCg                                                                                                   >  5:295627/1‑148 (MQ=255)                                                    cGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGca                                                                                                 >  3:3393/1‑148 (MQ=255)                                                        aTGCTCGGGCCGACGGGACTCGGCGCCTGGGGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCGGGCTTCACAGGGTCGTGACCTCGCTTAACCCGCAACaaa                                                                                            <  6:295627/149‑1 (MQ=255)                                                                gCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGcaa                                                                                                >  4:34847/1‑137 (MQ=255)                                                                  cGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAAt                                                                                  <  3:208221/149‑1 (MQ=255)                                                                    aCGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTg                                                                                                                  >  5:55012/1‑115 (MQ=255)                                                                    aCGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTg                                                                                                                  <  6:55012/115‑1 (MQ=255)                                                                      ggTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGc                                                                                >  1:54377/1‑147 (MQ=255)                                                                        tACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAgg                                                                            <  2:58486/149‑1 (MQ=255)                                                                           tCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAg                                                                                       <  6:329333/135‑1 (MQ=255)                                                                           tCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAg                                                                                       >  5:329333/1‑135 (MQ=255)                                                                             ggTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAg                                                                       >  7:278856/1‑149 (MQ=255)                                                                             ggTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAg                                                                       >  4:168288/1‑149 (MQ=255)                                                                              gTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGc                                                                      <  8:147510/149‑1 (MQ=255)                                                                                   tggtggGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCt                                                                                                          >  7:223980/1‑108 (MQ=255)                                                                                   tggtggGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCt                                                                                                          <  8:223980/108‑1 (MQ=255)                                                                                       ggggCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAAt                                                                                  <  2:202464/128‑1 (MQ=255)                                                                                       ggggCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAAt                                                                                  >  1:202464/1‑128 (MQ=255)                                                                                        gggCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGa                                                            <  1:325562/149‑1 (MQ=255)                                                                                        gggCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGa                                                            >  5:132544/1‑149 (MQ=255)                                                                                             cccGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACa                                                       >  5:347744/1‑149 (MQ=255)                                                                                               cGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGcaac                                                                                               <  1:137155/107‑1 (MQ=255)                                                                                               cGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGcaac                                                                                               >  2:137155/1‑107 (MQ=255)                                                                                                gCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATtg                                                    <  3:168288/149‑1 (MQ=255)                                                                                                 cATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACAATGACATtga                                                   <  7:69094/149‑1 (MQ=255)                                                                                                   tgctgcGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATtgatg                                                 <  2:177644/149‑1 (MQ=255)                                                                                                      tgcGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGatgatg                                              >  2:21702/1‑149 (MQ=255)                                                                                                       gcGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGt                                             >  3:103127/1‑149 (MQ=255)                                                                                                       gcGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGt                                             >  2:233937/1‑149 (MQ=255)                                                                                                          ttGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGa                                          <  8:278856/149‑1 (MQ=255)                                                                                                            ggTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGAt                                         <  1:369294/148‑1 (MQ=255)                                                                                                              agctcTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACTTACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATgaa                                      <  1:100354/145‑1 (MQ=255)                                                                                                                   tGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGCCCTCGCTTAACCCGCAACAAACCCAGCCAATGCCAGGTACAGCTTCATCACGATGACATTGATGATGTAGATgaa                                      >  2:100354/1‑144 (MQ=255)                                                                                                                       ccGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGcccc                             <  4:3393/149‑1 (MQ=255)                                                                                                                       ccGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGcccc                             <  6:347744/149‑1 (MQ=255)                                                                                                                        cGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTg                                                                   >  8:324328/1‑110 (MQ=255)                                                                                                                        cGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTg                                                                   <  7:324328/110‑1 (MQ=255)                                                                                                                         ggCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCcca                           >  5:298889/1‑149 (MQ=255)                                                                                                                               ccccACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCg                     >  4:292512/1‑149 (MQ=255)                                                                                                                                  cACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCGGCACCAACCCAAGGCAATGCCAGGTACCGGTTGATCCCGATGACATTGATGATGTCGATGAAGAACGCCCCCCCCATCGGcc                  >  3:104253/1‑148 (MQ=255)                                                                                                                                  cACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGca                  >  6:160422/1‑149 (MQ=255)                                                                                                                                   aCGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGcac                 <  4:103127/149‑1 (MQ=255)                                                                                                                                       caatCAGAACGTCGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGt                                             >  2:35114/4‑117 (MQ=255)                                                                                                                                       caatCAGAACGTCGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGt                                             <  1:35114/114‑1 (MQ=255)                                                                                                                                        aatCAGAACGTCGTTCTGAGTTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGcaccac              >  6:301278/3‑148 (MQ=255)                                                                                                                                            aGACCGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCTGGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGa         <  7:248203/148‑1 (MQ=255)                                                                                                                                            aGAACGTAGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGa         <  4:275941/148‑1 (MQ=255)                                                                                                                                            aGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGa         <  2:197943/148‑1 (MQ=255)                                                                                                                                                  acgTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGAAGGCGAg  <  2:54377/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                      CCTGCATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGA‑TTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGAAGGCGAG  >  NC_002947/1133944‑1134237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑