Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,135,614 T→A 13.2% E454V (GAA→GTA) ‡ PP_0997 ← sigma‑54 dependent transcriptional regulator/sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,135,6140TA13.2% 154.9 / 10.7 59E454V (GAA→GTA) ‡PP_0997sigma‑54 dependent transcriptional regulator/sensory box protein
Reads supporting (aligned to +/- strand):  ref base T (30/21);  new base A (4/4);  total (34/25)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGGG  >  NC_002947/1135466‑1135760
                                                                                                                                                    |                                                                                                                                                   
ttATGGTTTCCCAGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGtt                                                                                                                                                     >  7:191061/1‑149 (MQ=255)
   tGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGGGAAGTTTGCTAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTcct                                                                                                                                                  <  5:94213/149‑2 (MQ=255)
   tGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTcc                                                                                                                                                   <  5:202196/148‑1 (MQ=255)
     gTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAg                                                                                                                                                 <  2:202910/148‑1 (MQ=255)
          ccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCTCTCGAAATCACCTACAGCCTGTTCCAGGCTGGc                                                                                                                                           <  4:332956/149‑1 (MQ=255)
          ccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgt                                                                                                                                            <  2:90460/148‑2 (MQ=255)
          ccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgt                                                                                                                                            <  6:47720/148‑2 (MQ=255)
            gggTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGcc                                                                                                                                          >  8:319048/1‑148 (MQ=255)
              gTATTCCATATTTACGCAGCCTCTGCGAGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACt                                                                                                                                       >  3:209583/1‑149 (MQ=255)
                   ccATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtagg                                                                                                                                         >  6:206600/1‑138 (MQ=255)
                   ccATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtagg                                                                                                                                         <  5:206600/142‑5 (MQ=255)
                    cATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCg                                                                                                                                 <  5:7023/149‑1 (MQ=255)
                        tttACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCt                                                                                                                              <  1:293697/148‑1 (MQ=255)
                           aCGCAGCCTCTGCGCGATAGCCGTGTGCGAAGGTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGcc                                                                                                                          >  8:155087/1‑149 (MQ=255)
                             gCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCcg                                                                                                                        >  5:338294/1‑149 (MQ=255)
                                gCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGt                                                                                                                     <  8:191061/149‑1 (MQ=255)
                                gCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGt                                                                                                                     >  6:306040/1‑149 (MQ=255)
                                 ccTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGTCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtag                                                                                                                                          >  4:207164/1‑124 (MQ=255)
                                 ccTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTg                                                                                                                    >  2:259343/1‑149 (MQ=255)
                                 ccTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtag                                                                                                                                          <  3:207164/127‑4 (MQ=255)
                                          gATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTAGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAgg                                                                                                           >  5:219809/1‑149 (MQ=255)
                                                 gtgtGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGc                                                                                                    <  8:148323/149‑1 (MQ=255)
                                                       gAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTg                                                                                                                                             >  6:360579/1‑102 (MQ=255)
                                                       gAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTg                                                                                                                                             <  5:360579/102‑1 (MQ=255)
                                                       gAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATc                                                                                              <  1:100233/149‑1 (MQ=255)
                                                       gAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATc                                                                                              <  1:259343/149‑1 (MQ=255)
                                                          gTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAg                                                                                          <  7:38273/149‑1 (MQ=255)
                                                           tttGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTg                                                                                                                    <  8:125134/123‑1 (MQ=255)
                                                           tttGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTg                                                                                                                    >  7:125134/1‑123 (MQ=255)
                                                            ttGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGt                                                                                        >  8:174010/1‑149 (MQ=255)
                                                             tGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGt                                                                                        <  6:338294/148‑1 (MQ=255)
                                                                            aGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAg                                                                        <  7:155087/149‑1 (MQ=255)
                                                                                gCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGtt                                                                     <  1:283181/148‑1 (MQ=255)
                                                                                    tgtTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCt                                                                >  8:148489/1‑149 (MQ=255)
                                                                                        gACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGa                                                                                                  >  4:219051/1‑112 (MQ=255)
                                                                                        gACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGa                                                                                                  <  3:219051/112‑1 (MQ=255)
                                                                                              tAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGc                                                      >  1:212008/1‑149 (MQ=255)
                                                                                                  tGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcg                                                  >  6:20652/1‑149 (MQ=255)
                                                                                                           aGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGaa                                          <  7:319048/148‑1 (MQ=255)
                                                                                                            gcgcTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcgcgg                                            >  3:17713/1‑145 (MQ=255)
                                                                                                             cgcTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGa                                        >  6:199244/1‑148 (MQ=255)
                                                                                                                tGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGAACTGGGAATATCAGGGGGCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCa                                     >  1:304225/1‑149 (MQ=255)
                                                                                                                     cAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCt                               >  6:239446/1‑149 (MQ=255)
                                                                                                                      aGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTg                              <  5:239446/149‑1 (MQ=255)
                                                                                                                            cgcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTg                        >  2:191577/1‑149 (MQ=255)
                                                                                                                             gcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTgg                       >  4:152848/1‑149 (MQ=255)
                                                                                                                               tCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGgcg                     >  8:293942/1‑149 (MQ=255)
                                                                                                                               tCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGgcg                     >  2:120728/1‑149 (MQ=255)
                                                                                                                                 gAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGca                   >  7:221917/1‑149 (MQ=255)
                                                                                                                                     tCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACAtt               <  5:306040/149‑1 (MQ=255)
                                                                                                                                       aCCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGc             >  6:86352/1‑149 (MQ=255)
                                                                                                                                          tACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  1:181865/1‑149 (MQ=255)
                                                                                                                                          tACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  3:311036/1‑149 (MQ=255)
                                                                                                                                          tACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  3:305957/1‑149 (MQ=255)
                                                                                                                                          gccAGCCTGGAACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  2:78364/3‑149 (MQ=255)
                                                                                                                                          gccAGCCTGGAACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  8:52085/3‑149 (MQ=255)
                                                                                                                                             aGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGc        >  8:259926/1‑148 (MQ=255)
                                                                                                                                               ccTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGcc       >  8:119582/1‑147 (MQ=255)
                                                                                                                                               ccTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCaa     <  7:293942/149‑1 (MQ=255)
                                                                                                                                                 tGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAgg   >  5:244956/1‑149 (MQ=255)
                                                                                                                                                   ttCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAgg   <  5:86352/147‑1 (MQ=255)
                                                                                                                                                   ttCCAGGCTGGCCACTGCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAggg  <  4:209583/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTATGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGGG  >  NC_002947/1135466‑1135760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑