Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,135,615	C→A	13.4%	E454* (GAA→TAA) ‡	PP_0997 ←	sigma‑54 dependent transcriptional regulator/sensory box protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,135,615	0	C	A	13.4%	163.0 / 12.9	59	E454* (GAA→TAA) ‡	PP_0997	sigma‑54 dependent transcriptional regulator/sensory box protein
Reads supporting (aligned to +/- strand):  ref base C (30/21);  new base A (4/4);  total (34/25)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGGG  >  NC_002947/1135469‑1135760                                                                                                                                                   |                                                                                                                                                   tGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGGGAAGTTTGCTAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTcct                                                                                                                                                  <  5:94213/149‑2 (MQ=255) tGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTcc                                                                                                                                                   <  5:202196/148‑1 (MQ=255)   gTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAg                                                                                                                                                 <  2:202910/148‑1 (MQ=255)        ccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCTCTCGAAATCACCTACAGCCTGTTCCAGGCTGGc                                                                                                                                           <  4:332956/149‑1 (MQ=255)        ccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgt                                                                                                                                            <  2:90460/148‑2 (MQ=255)        ccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgt                                                                                                                                            <  6:47720/148‑2 (MQ=255)          gggTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGcc                                                                                                                                          >  8:319048/1‑148 (MQ=255)            gTATTCCATATTTACGCAGCCTCTGCGAGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACt                                                                                                                                       >  3:209583/1‑149 (MQ=255)                 ccATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtagg                                                                                                                                         >  6:206600/1‑138 (MQ=255)                 ccATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtagg                                                                                                                                         <  5:206600/142‑5 (MQ=255)                  cATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCg                                                                                                                                 <  5:7023/149‑1 (MQ=255)                      tttACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCt                                                                                                                              <  1:293697/148‑1 (MQ=255)                         aCGCAGCCTCTGCGCGATAGCCGTGTGCGAAGGTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGcc                                                                                                                          >  8:155087/1‑149 (MQ=255)                           gCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCcg                                                                                                                        >  5:338294/1‑149 (MQ=255)                              gCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGt                                                                                                                     >  6:306040/1‑149 (MQ=255)                              gCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGt                                                                                                                     <  8:191061/149‑1 (MQ=255)                               ccTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGTCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtag                                                                                                                                          >  4:207164/1‑124 (MQ=255)                               ccTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTg                                                                                                                    >  2:259343/1‑149 (MQ=255)                               ccTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtag                                                                                                                                          <  3:207164/127‑4 (MQ=255)                                        gATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTAGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAgg                                                                                                           >  5:219809/1‑149 (MQ=255)                                               gtgtGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGc                                                                                                    <  8:148323/149‑1 (MQ=255)                                                     gAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTg                                                                                                                                             <  5:360579/102‑1 (MQ=255)                                                     gAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTg                                                                                                                                             >  6:360579/1‑102 (MQ=255)                                                     gAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATc                                                                                              <  1:100233/149‑1 (MQ=255)                                                     gAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATc                                                                                              <  1:259343/149‑1 (MQ=255)                                                        gTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAg                                                                                          <  7:38273/149‑1 (MQ=255)                                                         tttGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTg                                                                                                                    <  8:125134/123‑1 (MQ=255)                                                         tttGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTg                                                                                                                    >  7:125134/1‑123 (MQ=255)                                                          ttGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGt                                                                                        >  8:174010/1‑149 (MQ=255)                                                           tGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGt                                                                                        <  6:338294/148‑1 (MQ=255)                                                                          aGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAg                                                                        <  7:155087/149‑1 (MQ=255)                                                                              gCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGtt                                                                     <  1:283181/148‑1 (MQ=255)                                                                                  tgtTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCt                                                                >  8:148489/1‑149 (MQ=255)                                                                                      gACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGa                                                                                                  >  4:219051/1‑112 (MQ=255)                                                                                      gACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGa                                                                                                  <  3:219051/112‑1 (MQ=255)                                                                                            tAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGc                                                      >  1:212008/1‑149 (MQ=255)                                                                                                tGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcg                                                  >  6:20652/1‑149 (MQ=255)                                                                                                         aGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGaa                                          <  7:319048/148‑1 (MQ=255)                                                                                                          gcgcTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcgcgg                                            >  3:17713/1‑145 (MQ=255)                                                                                                           cgcTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGa                                        >  6:199244/1‑148 (MQ=255)                                                                                                              tGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGAACTGGGAATATCAGGGGGCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCa                                     >  1:304225/1‑149 (MQ=255)                                                                                                                   cAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCt                               >  6:239446/1‑149 (MQ=255)                                                                                                                    aGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTg                              <  5:239446/149‑1 (MQ=255)                                                                                                                          cgcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTg                        >  2:191577/1‑149 (MQ=255)                                                                                                                           gcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTgg                       >  4:152848/1‑149 (MQ=255)                                                                                                                             tCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGgcg                     >  8:293942/1‑149 (MQ=255)                                                                                                                             tCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGgcg                     >  2:120728/1‑149 (MQ=255)                                                                                                                               gAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGca                   >  7:221917/1‑149 (MQ=255)                                                                                                                                   tCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACAtt               <  5:306040/149‑1 (MQ=255)                                                                                                                                     aCCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGc             >  6:86352/1‑149 (MQ=255)                                                                                                                                        tACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  1:181865/1‑149 (MQ=255)                                                                                                                                        tACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  3:311036/1‑149 (MQ=255)                                                                                                                                        tACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  3:305957/1‑149 (MQ=255)                                                                                                                                        gccAGCCTGGAACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  2:78364/3‑149 (MQ=255)                                                                                                                                        gccAGCCTGGAACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  8:52085/3‑149 (MQ=255)                                                                                                                                           aGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGc        >  8:259926/1‑148 (MQ=255)                                                                                                                                             ccTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGcc       >  8:119582/1‑147 (MQ=255)                                                                                                                                             ccTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCaa     <  7:293942/149‑1 (MQ=255)                                                                                                                                               tGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAgg   >  5:244956/1‑149 (MQ=255)                                                                                                                                                 ttCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAgg   <  5:86352/147‑1 (MQ=255)                                                                                                                                                 ttCCAGGCTGGCCACTGCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAggg  <  4:209583/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   TGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATC‑CAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGGG  >  NC_002947/1135469‑1135760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑