Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,201,587 T→A 7.5% L132Q (CTG→CAG)  xcpV → type II secretion pathway protein XcpV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,201,5870TA 7.5% 194.7 / 4.5 66L132Q (CTG→CAG) xcpVtype II secretion pathway protein XcpV
Reads supporting (aligned to +/- strand):  ref base T (30/31);  new base A (2/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TAGCCGGCGAGCTCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCAGGACAC  >  NC_002947/1201440‑1201727
                                                                                                                                                   |                                                                                                                                            
tAGCCGGCGAGCTCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTg                                                                                                                                             <  7:178874/149‑1 (MQ=255)
 aGCCGGCGAGCTCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTg                                                                                                                                             >  1:192460/1‑148 (MQ=255)
    cGGCGAGCTCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAgc                                                                                                                                         <  6:296826/149‑1 (MQ=255)
     ggCGAGCTCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAgcg                                                                                                                                        >  1:252077/1‑149 (MQ=255)
            tCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACAAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATg                                                                                                                                  >  3:233175/1‑148 (MQ=255)
              ggCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGaaa                                                                                                                               >  4:180796/1‑149 (MQ=255)
              ggCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGaaa                                                                                                                               <  2:252077/149‑1 (MQ=255)
               gCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAac                                                                                                                              <  7:114191/149‑1 (MQ=255)
                ccAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAaca                                                                                                                             >  5:43132/1‑149 (MQ=255)
                  aaGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCATGGGCGCTCCAGAAacacg                                                                                                                           <  8:245565/149‑2 (MQ=255)
                  aaGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCATGGGCGCTCCAGAAacacg                                                                                                                           <  5:83526/149‑2 (MQ=255)
                  aaGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCATGGGCGCTCCAGAAacacg                                                                                                                           <  4:283061/149‑2 (MQ=255)
                    ggTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGc                                                                                                                         <  3:35378/149‑1 (MQ=255)
                            gCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCgg                                                                                                                 >  2:55244/1‑149 (MQ=255)
                                    aCTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGAccc                                                                                                         >  3:130691/1‑149 (MQ=255)
                                         caactgcaactgCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATc                                                                                                    <  1:161095/149‑1 (MQ=255)
                                         caactgcaactgCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATc                                                                                                    <  6:314157/149‑1 (MQ=255)
                                           actgcaactgcGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCg                                                                                                   >  7:16892/1‑148 (MQ=255)
                                            ctgcaactgcGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGaa                                                                                                 <  3:147107/149‑1 (MQ=255)
                                               caactgcGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTg                                                                                              <  1:55244/149‑1 (MQ=255)
                                                 actgcGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGAt                                                                                            <  5:115541/149‑1 (MQ=255)
                                                  ctgcGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACAAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATg                                                                                           <  4:233175/149‑1 (MQ=255)
                                                     cgccgcACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTc                                                                                        <  8:16892/149‑1 (MQ=255)
                                                       ccgcACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgc                                                                                      >  5:49627/1‑149 (MQ=255)
                                                         gcACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGc                                                                                                                         <  6:160259/112‑1 (MQ=255)
                                                         gcACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGc                                                                                                                         >  5:160259/1‑112 (MQ=255)
                                                           aCCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgccctg                                                                                  >  5:310609/1‑149 (MQ=255)
                                                              ggCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgccctggcc                                                                               <  6:49627/149‑1 (MQ=255)
                                                               gCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgccctggcc                                                                               >  8:195792/1‑148 (MQ=255)
                                                                 gTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgccctggccctg                                                                            <  3:304514/149‑1 (MQ=255)
                                                                   gCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGAc                                                                          >  5:179350/1‑149 (MQ=255)
                                                                    cGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACt                                                                         >  4:307862/1‑149 (MQ=255)
                                                                      agaCCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGc                                                                       <  3:180796/149‑1 (MQ=255)
                                                                        aCCTGCGCCGTGGCGAACTGCAAATGTTCGACGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCg                                                                     >  6:102243/1‑149 (MQ=255)
                                                                          cTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGtt                                                                   >  2:202818/1‑149 (MQ=255)
                                                                             cgcCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCtt                                                                <  2:192460/149‑1 (MQ=255)
                                                                             cgcCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCtt                                                                <  4:130691/149‑1 (MQ=255)
                                                                             cgcCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCt                                                                 <  6:310609/148‑1 (MQ=255)
                                                                                 gTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGca                                                            <  6:179350/149‑1 (MQ=255)
                                                                                         cTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAATACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTc                                                    >  5:125115/1‑149 (MQ=255)
                                                                                           gCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTcgc                                                  >  1:319656/1‑149 (MQ=255)
                                                                                            cAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTcgcc                                                 >  8:30316/1‑149 (MQ=255)
                                                                                             aaTTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTcgccg                                                >  4:157458/1‑149 (MQ=255)
                                                                                              aTTGTTCGCCGCCCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTcgccgc                                               >  1:134599/1‑149 (MQ=255)
                                                                                              aTTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACGGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTcgccgc                                               >  1:260289/1‑149 (MQ=255)
                                                                                                 gtgcGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCgcgc                                             <  2:62536/145‑1 (MQ=255)
                                                                                                 gTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCt                                            >  3:26747/1‑149 (MQ=255)
                                                                                                  ttCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTg                                           <  7:30316/149‑1 (MQ=255)
                                                                                                     gCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgccctggccct                                                                             >  2:6470/1‑112 (MQ=255)
                                                                                                     gCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgccctggccct                                                                             <  1:6470/112‑1 (MQ=255)
                                                                                                           cgcgACCAGCGCCTCCCACTGGGCCAGTACACCGTGTTTCAGGAGCGCCCATGAAACACCGCCACACCGGCCTGACCCTGATCGAACTGCTGGTCGCCCTGGCCCTCACTGCCCTGCTTGCCATCATGCTCGCCGCGCTGGTCAAtcgc                                  >  3:34619/1‑149 (MQ=255)
                                                                                                             cgACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTg                                                               >  2:334017/1‑118 (MQ=255)
                                                                                                             cgACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTg                                                               <  1:334017/118‑1 (MQ=255)
                                                                                                                    ctccTGCCCCTCGGCCAGTCCCCCGTCGTTCTGCCCCGCCCCTGCAACACCGCCCACCCGGCCTGCCCCTGCTCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCCGGTCAATGGCTGGCTGAAg                         <  4:34619/147‑1 (MQ=255)
                                                                                                                    cgcCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAg                         <  2:134599/149‑1 (MQ=255)
                                                                                                                         gCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCa                                       >  2:243637/1‑130 (MQ=255)
                                                                                                                         gCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCa                                       <  1:243637/130‑1 (MQ=255)
                                                                                                                          ccACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTgcgc                   <  3:157458/149‑1 (MQ=255)
                                                                                                                           cACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTgcgc                   >  1:365383/1‑148 (MQ=255)
                                                                                                                                   cAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTg          <  5:102243/149‑1 (MQ=255)
                                                                                                                                     atACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCa        <  6:125115/148‑1 (MQ=255)
                                                                                                                                         gcggtgtTTCATGGGCGCTCCAGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCAGGa     >  4:32554/4‑148 (MQ=255)
                                                                                                                                         acCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCAGGa     >  5:349048/1‑148 (MQ=255)
                                                                                                                                          ccGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCa        <  4:67497/144‑1 (MQ=255)
                                                                                                                                          ccGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCa        >  3:67497/1‑144 (MQ=255)
                                                                                                                                           cGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCAGGacac  >  6:32593/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
TAGCCGGCGAGCTCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCAGGACAC  >  NC_002947/1201440‑1201727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑