Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,233,768 T→A 8.8% intergenic (‑84/+18) glpR ← / ← glpK DNA‑binding transcriptional repressor GlpR/glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,233,7680TA 8.8% 159.2 / 5.3 57intergenic (‑84/+18)glpR/glpKDNA‑binding transcriptional repressor GlpR/glycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (24/28);  new base A (2/3);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

CTGACATAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGTCGCGCAGC  >  NC_002947/1233623‑1233915
                                                                                                                                                 |                                                                                                                                                   
cTGACATAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATggc                                                                                                                                                  <  4:18707/149‑1 (MQ=255)
cTGACATAGCCGCGTTCGCGTACCAGCTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATggc                                                                                                                                                  >  3:36515/1‑149 (MQ=255)
 tGACATAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATggcg                                                                                                                                                 >  5:23136/1‑149 (MQ=255)
 tGACATAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTACCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATggcg                                                                                                                                                 >  1:17633/1‑149 (MQ=255)
  gACATAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATggcgg                                                                                                                                                <  3:138378/149‑1 (MQ=255)
   aCATAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATggcggc                                                                                                                                               >  8:117385/1‑149 (MQ=255)
      tAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCCATGCGGcccc                                                                                                                                            <  6:298450/149‑1 (MQ=255)
       aGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATTATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCCTGGCGGcccc                                                                                                                                            >  8:243720/1‑148 (MQ=255)
        gCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCCATGCGGCCCCaa                                                                                                                                          <  2:56894/149‑1 (MQ=255)
          cgcgTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACa                                                                                                                                        <  1:60146/149‑1 (MQ=255)
             gTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCt                                                                                                                                     <  2:325079/149‑1 (MQ=255)
                           tCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCATTGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCCATGCGGCCCCaatc                                                                                                                                        <  4:46347/132‑3 (MQ=255)
                           tCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCCATGCGGCCCCaatc                                                                                                                                        >  3:46347/1‑130 (MQ=255)
                             gAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATc                                                                                                                     >  3:108676/1‑149 (MQ=255)
                                 aTTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTcc                                                                                                                 <  2:10152/149‑1 (MQ=255)
                                   ttgttgttgGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTTATCTTccc                                                                                                                <  7:39453/148‑1 (MQ=255)
                                              gggggggCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGcc                                                                                                                                              <  1:138148/107‑1 (MQ=255)
                                              gggggggCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGcc                                                                                                                                              >  2:138148/1‑107 (MQ=255)
                                               ggggggCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACg                                                                                                   >  4:17707/1‑149 (MQ=255)
                                                  gggCAGATTCATGGGCGTTCCTTTGGGGCTGCCGTACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTc                                                                                                <  4:49200/149‑1 (MQ=255)
                                                   ggCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCaa                                                                                                                                          <  5:158192/106‑1 (MQ=255)
                                                   ggCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCaa                                                                                                                                          >  6:158192/1‑106 (MQ=255)
                                                    gCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGa                                                                                              >  2:133762/1‑149 (MQ=255)
                                                      aGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGAcc                                                                                            >  3:303387/1‑149 (MQ=255)
                                                            aTGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCcttc                                                                                      <  7:189214/149‑1 (MQ=255)
                                                            aTGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCcttc                                                                                      <  4:303387/149‑1 (MQ=255)
                                                               tgCGGGCCTTTGGGGCTGCCGGACAAATTCTGCACTGATGCCGTTGGAAGGGGATGACGTCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTcc                                                                                                                 <  5:365773/118‑1 (MQ=255)
                                                               ggCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACCATTCGGGCCCCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTcc                                                                                                                 >  6:365773/1‑119 (MQ=255)
                                                               ggCGGTCCTTTGGGGCTACCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCttcttc                                                                                   <  2:17633/149‑1 (MQ=255)
                                                                gCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCttcttc                                                                                   >  3:331263/1‑148 (MQ=255)
                                                                gCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCttcttc                                                                                   <  4:331263/148‑1 (MQ=255)
                                                                  ggTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAg                                                                                >  2:182753/1‑149 (MQ=255)
                                                                   gTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGc                                                                               >  6:67278/1‑149 (MQ=255)
                                                                             gCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAg                                                                     <  6:23136/149‑1 (MQ=255)
                                                                                   ggACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTtttc                                                               >  4:80913/1‑149 (MQ=255)
                                                                                       aaaTTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTtttctttc                                                           <  6:147872/149‑1 (MQ=255)
                                                                                        aaTTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTttctttct                                                          <  4:108676/149‑1 (MQ=255)
                                                                                          ttCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGc                                                        >  7:196223/1‑149 (MQ=255)
                                                                                          ttCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGc                                                        <  3:158719/149‑1 (MQ=255)
                                                                                                       ccGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGtt                                           >  1:54411/1‑149 (MQ=255)
                                                                                                       ccGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGtt                                           >  1:59712/1‑149 (MQ=255)
                                                                                                        cGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTg                                          >  3:73973/1‑149 (MQ=255)
                                                                                                         gTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGc                                         <  5:67278/149‑1 (MQ=255)
                                                                                                         gTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGc                                         >  3:315649/1‑149 (MQ=255)
                                                                                                            ggAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCggg                                      >  3:39323/1‑149 (MQ=255)
                                                                                                                   gATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATATTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACt                               >  6:202654/1‑149 (MQ=255)
                                                                                                                      gACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCAc                            >  8:41104/1‑149 (MQ=255)
                                                                                                                        cGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGc                          <  1:133762/149‑1 (MQ=255)
                                                                                                                                 gACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATc                 <  4:73973/149‑1 (MQ=255)
                                                                                                                                 gACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATc                 <  4:36515/149‑1 (MQ=255)
                                                                                                                                 gACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATc                 <  1:182753/149‑1 (MQ=255)
                                                                                                                                      ttGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCtt            <  3:17707/149‑1 (MQ=255)
                                                                                                                                        ggggCCGCCATGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGt          >  1:11390/1‑149 (MQ=255)
                                                                                                                                         gggCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGTc         <  7:243720/149‑1 (MQ=255)
                                                                                                                                            ccGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGTcgcg      <  2:59712/149‑1 (MQ=255)
                                                                                                                                            ccGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGTcgcg      <  2:54411/149‑1 (MQ=255)
                                                                                                                                                aTGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGTCGCGCAGc  <  7:41104/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CTGACATAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGTCGCGCAGC  >  NC_002947/1233623‑1233915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑