Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,267,271 C→T 7.0% P239P (CCG→CCA)  quiP ← acyl‑homoserine lactone acylase QuiP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,267,2710CT 7.0% 170.6 / 2.3 57P239P (CCG→CCA) quiPacyl‑homoserine lactone acylase QuiP
Reads supporting (aligned to +/- strand):  ref base C (29/24);  new base T (2/2);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AGGCGGCGCGGCTGTCGCTGGCCAGCAGGCTCTTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCTGAGTTCCTCTTGCAGG  >  NC_002947/1267129‑1267418
                                                                                                                                              |                                                                                                                                                   
aGGCGGCGCGGCTGTCGCTGGCCAGCAGGCTCTTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAAc                                                                                                                                               <  8:239491/149‑1 (MQ=255)
       gcggcTGTCGCTGGCCAGCAGGCTCTTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAActggctgg                                                                                                                                        >  1:280133/1‑149 (MQ=255)
          gcTGTCGCTGGCCAGCAGGCTCTTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACtggctggc                                                                                                                                       >  7:214314/1‑147 (MQ=255)
           ctgaaccTGGCCAGCAGGGTCTTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGTCAGCCCCGGCAACTGGCTGGCCAg                                                                                                                                    <  3:244739/143‑1 (MQ=255)
                 cTGGCCAGCAGGCTCTTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAg                                                                                                                              <  2:220822/149‑1 (MQ=255)
                      cagcagGCTCTTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCt                                                                                                                         <  4:259397/149‑1 (MQ=255)
                      cagcagGCTCTTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCt                                                                                                                         >  5:149556/1‑149 (MQ=255)
                       agcagGCTCTTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCtt                                                                                                                        <  5:46375/149‑1 (MQ=255)
                             ctctTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCtt                                                                                                                  <  8:214314/149‑1 (MQ=255)
                              tctTGCCGCTGCGGCGGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTg                                                                                                                 <  2:176605/149‑1 (MQ=255)
                                ttGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTc                                                                                                               <  8:270386/149‑1 (MQ=255)
                                  gCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGt                                                                                                                <  6:279216/146‑1 (MQ=255)
                                    cGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTc                                                                                                               >  6:291821/1‑145 (MQ=255)
                                    cGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTc                                                                                                               <  5:291821/145‑1 (MQ=255)
                                          ggctgcgCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGACAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCAtt                                                                                                     >  1:193396/1‑149 (MQ=255)
                                              gcgctgcgGACCCCGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCGTGAGCTTGTCCACTTCCATTTCgg                                                                                                 >  5:302474/1‑149 (MQ=255)
                                                  tgcgGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCaa                                                                                             >  5:372190/1‑149 (MQ=255)
                                                   gcgGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAAc                                                                                            >  2:366471/1‑149 (MQ=255)
                                                        cccAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGGCCACTTCCATTTCGGCCAACGGCTc                                                                                       >  6:262335/1‑149 (MQ=255)
                                                         ccAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCg                                                                                      >  8:29214/1‑149 (MQ=255)
                                                               ccAGGTTGGCCGACCCCGGGCTACCGCGCAGGTCGAGGTCGGCCAGCTTCTGGCGGGCAGCCGTCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCggg                                                                                <  4:206934/149‑1 (MQ=255)
                                                                cAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCgggg                                                                               >  5:373787/1‑149 (MQ=255)
                                                                   gTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGCCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGccc                                                                            <  2:280133/149‑1 (MQ=255)
                                                                      ggCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCg                                                                          <  6:374703/148‑1 (MQ=255)
                                                                       gCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCgg                                                                         <  6:149556/148‑1 (MQ=255)
                                                                         cGACCCCGGGCTACCGAGCAGGTCGCGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGgca                                                                      <  7:267923/149‑1 (MQ=255)
                                                                         cGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGgca                                                                      <  6:71420/149‑1 (MQ=255)
                                                                             cccGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGgca                                                                      >  7:153791/1‑145 (MQ=255)
                                                                             cccGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGgca                                                                      <  8:153791/145‑1 (MQ=255)
                                                                               cGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAAc                                                                 >  4:51334/1‑148 (MQ=255)
                                                                                gggCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCa                                                               <  6:373787/149‑1 (MQ=255)
                                                                                  gCTACCTAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCAAGGTTCAGGCCCTTGAGCTTGTCAAATTCCATTTCGGACAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAgg                                                             >  2:200735/1‑149 (MQ=255)
                                                                                    tACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGc                                                            >  3:360853/1‑148 (MQ=255)
                                                                                    tACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGc                                                            >  2:308715/1‑148 (MQ=255)
                                                                                       cGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCTCTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGc                                                        >  1:302248/1‑149 (MQ=255)
                                                                                       cGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGc                                                        >  7:139539/1‑149 (MQ=255)
                                                                                        gAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCt                                                       >  5:166393/1‑149 (MQ=255)
                                                                                           cAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGt                                                    <  6:372190/149‑1 (MQ=255)
                                                                                               tcgaggtcgGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGc                                                 >  4:102185/1‑148 (MQ=255)
                                                                                               tcgaggtcgGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGc                                                 >  4:97082/1‑148 (MQ=255)
                                                                                                  aggtcgGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTc                                                                                       <  5:367830/107‑1 (MQ=255)
                                                                                                  aggtcgGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTc                                                                                       >  6:367830/1‑107 (MQ=255)
                                                                                                  aggtcgGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTAcc                                             <  2:193396/149‑1 (MQ=255)
                                                                                                         ccAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCGACCGGCTTTc                                      >  2:53836/1‑149 (MQ=255)
                                                                                                          cAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCt                                     <  1:179642/149‑1 (MQ=255)
                                                                                                             cTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTgcg                                  >  4:140321/1‑149 (MQ=255)
                                                                                                              ttCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTgcgc                                 >  2:300459/1‑149 (MQ=255)
                                                                                                                   gctggcAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCACGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAggg                            >  5:199107/1‑149 (MQ=255)
                                                                                                                     tggcAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTATGCGCCAGGGt                           >  5:246890/1‑148 (MQ=255)
                                                                                                                          gttgccGGGGCTGCCAGCCCTGGCAACTGGCTTGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGgca                                                                      <  6:171274/97‑1 (MQ=255)
                                                                                                                          gttgccGGGGCTGCCAGCCCTGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGgc                     >  4:354422/4‑149 (MQ=255)
                                                                                                                          gttgccGGGGCTGCCAGCCCTGGCAACTGGCTGGCCAGGGTCAGGACCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCAGGgca                                                                      >  5:171274/4‑100 (MQ=255)
                                                                                                                             gccAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCt                  <  3:51334/149‑1 (MQ=255)
                                                                                                                               aaGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTGGTCCACTTCCACTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCTGa                <  7:194844/148‑1 (MQ=255)
                                                                                                                                    cTGCCAGCCCTGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCTGAGTTcc           <  3:354422/149‑1 (MQ=255)
                                                                                                                                      ggCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCTGAGTTCctc         >  2:318823/1‑149 (MQ=255)
                                                                                                                                       gCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCAGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCTGAGTTCctct        >  7:337986/1‑149 (MQ=255)
                                                                                                                                            cccGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCTGAGTTCCTCTTGCAg   <  1:308715/149‑1 (MQ=255)
                                                                                                                                            cccGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGACAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCTGAGTTCCTCTTGCAg   >  4:216101/1‑149 (MQ=255)
                                                                                                                                            cccGGCAACTGGCTGGCCAGGTTCAGGCCCATGAGCTTGTCCACTTCCATTTCGGCCACCGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCTGAGTTCCTCTTGCAg   >  1:34804/1‑149 (MQ=255)
                                                                                                                                             ccGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCTGAGTTCCTCTTGCAgg  <  1:366471/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
AGGCGGCGCGGCTGTCGCTGGCCAGCAGGCTCTTGCCGCTGCGGCTGCGCTGCGGACCCAGGGCCAGGTTGGCCGACCCCGGGCTACCGAGCAGGTCGAGGTCGGCCAGCTTCTGGCTGGCAGCCGCCAGGGCTGGCAGCCCCGGCAACTGGCTGGCCAGGTTCAGGCCCTTGAGCTTGTCCACTTCCATTTCGGCCAACGGCTCGTCCGGGGCCCCGGGCAGCAACCAGGCCAGCTTGTCGCTACCGGCTTTCTGCGCCAGGGTCAGGGCGCTGAGTTCCTCTTGCAGG  >  NC_002947/1267129‑1267418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑