Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,277,323 C→A 5.1% G16V (GGT→GTT) ‡ PP_1116 ← resolvase family site‑specific recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,277,3230CA 5.1% 250.7 / 3.4 78G16V (GGT→GTT) ‡PP_1116resolvase family site‑specific recombinase
Reads supporting (aligned to +/- strand):  ref base C (36/38);  new base A (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-01

GTCAGAGCTTTTGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTAGTGCAAATGGAAATTCTAG  >  NC_002947/1277176‑1277463
                                                                                                                                                   |                                                                                                                                            
gTCAGAGCTTTTGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCAc                                                                                                                                              <  7:242169/148‑1 (MQ=255)
 tCAGAGCTTTTGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCAcc                                                                                                                                             <  8:339785/148‑1 (MQ=255)
 tCAGAGCTTTTGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCt                                                                                                                                            >  1:32577/1‑149 (MQ=255)
 tCAGAGCTTTTGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCt                                                                                                                                            >  7:247046/1‑149 (MQ=255)
   agagCTTTTGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCtt                                                                                                                                           >  5:205329/1‑148 (MQ=255)
   agagCTTTTGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCtt                                                                                                                                           >  2:365108/1‑148 (MQ=255)
   agagCTTTTGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCtt                                                                                                                                           >  5:316801/1‑148 (MQ=255)
   agagCTTTTGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCtt                                                                                                                                           >  3:27477/1‑148 (MQ=255)
        ttttGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGActct                                                                                                                                     >  5:99630/1‑149 (MQ=255)
          ttGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCAAGGTGActctg                                                                                                                                    <  6:212477/148‑2 (MQ=255)
           tGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTg                                                                                                                                   <  1:95126/148‑1 (MQ=255)
            gCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTgg                                                                                                                                  <  7:252252/148‑1 (MQ=255)
             cACCTTCTTGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTa                                                                                                                                <  4:279967/149‑1 (MQ=255)
             cACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGa                                                                                                                                         >  3:367514/1‑140 (MQ=255)
             cACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGa                                                                                                                                         <  4:367514/140‑1 (MQ=255)
             cACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGt                                                                                                                                 <  2:32577/148‑1 (MQ=255)
              aCCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTgg                                                                                                                                  >  8:241255/1‑146 (MQ=255)
              aCCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTgg                                                                                                                                  >  8:143654/1‑146 (MQ=255)
              aCCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTgg                                                                                                                                  >  2:228105/1‑146 (MQ=255)
                cTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGa                                                                                                                              >  1:329572/1‑148 (MQ=255)
                   cTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATa                                                                                                                            <  6:242380/147‑1 (MQ=255)
                    tGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTc                                                                                                                         >  6:163117/1‑149 (MQ=255)
                     ggACGCTCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTc                                                                                                                         >  7:82979/1‑148 (MQ=255)
                      gACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTc                                                                                                                         <  1:128713/147‑1 (MQ=255)
                      gACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCAAAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCg                                                                                                                        >  6:329160/1‑148 (MQ=255)
                       aCGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGaa                                                                                                                      <  4:7086/149‑1 (MQ=255)
                       aCGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGaa                                                                                                                      <  6:153502/149‑1 (MQ=255)
                         gATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATg                                                                                                                    >  6:345416/1‑149 (MQ=255)
                               cAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATg                                                                                                                    <  7:241255/143‑1 (MQ=255)
                               cAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTaa                                                                                                                 <  1:228105/146‑1 (MQ=255)
                                  gagcgagcTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTaa                                                                                                                 >  6:230896/1‑143 (MQ=255)
                                  gagcgagcTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTaa                                                                                                                 <  5:230896/143‑1 (MQ=255)
                                  gagcgagcTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGcaac                                                                                                            >  8:211718/1‑148 (MQ=255)
                                    gcgagcTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCaacaac                                                                                                         <  5:329160/149‑1 (MQ=255)
                                        gcTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGttt                                                                                                     <  7:143654/149‑1 (MQ=255)
                                        gcTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGttt                                                                                                     <  1:365108/149‑1 (MQ=255)
                                          ttGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGt                                                                                                   >  1:107636/1‑149 (MQ=255)
                                               cGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCatga                                                                                              <  6:316801/149‑1 (MQ=255)
                                               cGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCatga                                                                                              <  8:247046/149‑1 (MQ=255)
                                                    ccACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCatgatgat                                                                                          <  6:205329/148‑1 (MQ=255)
                                                     cacaTAAACAAATTCACCAACGACATTCCAGTCGTTCGATTGGGCCGCACGTTGGATGTAATCACGCTCGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATAATCGAATGTAAGCAACAACGTTTGTCatgatgatg                                                                                         >  3:342907/1‑148 (MQ=255)
                                                      acaTCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGc                                                                                                               >  8:63397/1‑125 (MQ=255)
                                                      acaTCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGc                                                                                                               <  7:63397/125‑1 (MQ=255)
                                                       caTCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGa                                                                                                                              <  8:314372/109‑1 (MQ=255)
                                                       caTCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGa                                                                                                                              >  7:314372/1‑109 (MQ=255)
                                                           cacaAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGt                                                                                   <  2:329572/148‑1 (MQ=255)
                                                                  tCACCAACGACATTCCAGTCGTTCGCTAGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCATGGTAGATACTCGAATGTAAGCAACAACGGTTGTCAGGATGATGGCTCGTCTGTGAAt                                                                           >  4:220174/1‑149 (MQ=255)
                                                                    aCCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTTACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGa                                                                         <  5:300358/149‑1 (MQ=255)
                                                                    aCCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGa                                                                         <  6:99630/149‑1 (MQ=255)
                                                                    aCCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGa                                                                         <  4:27477/149‑1 (MQ=255)
                                                                       aaCGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGACTGTGTATGAGGc                                                                      >  3:136381/1‑149 (MQ=255)
                                                                          gACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCaa                                                                   >  1:93272/1‑149 (MQ=255)
                                                                                ccAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTAt                                                              <  7:211718/148‑1 (MQ=255)
                                                                                      gTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGc                                                       <  8:82979/149‑1 (MQ=255)
                                                                                          gCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCAt                                                    >  6:256867/1‑148 (MQ=255)
                                                                                           cTTGGGCCTCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGa                                                  <  3:332506/149‑1 (MQ=255)
                                                                                           cTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCAt                                                    >  4:9533/1‑147 (MQ=255)
                                                                                           cTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGa                                                  <  2:93272/149‑1 (MQ=255)
                                                                                           cTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGa                                                  <  5:50579/149‑1 (MQ=255)
                                                                                           cTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGa                                                  <  5:256867/149‑1 (MQ=255)
                                                                                           cTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGa                                                  <  3:9533/149‑1 (MQ=255)
                                                                                                gCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTAtt                                             >  2:98770/1‑149 (MQ=255)
                                                                                                  cGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTATGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTg                                           >  5:49877/1‑149 (MQ=255)
                                                                                                     aCGATGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTTTGAGGCCAAATTATCAACTGCCATGAGTATTTGTc                                         >  2:20123/1‑148 (MQ=255)
                                                                                                       gTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGACTGTGTATGAGGCCAAt                                                                  <  1:157494/121‑1 (MQ=255)
                                                                                                       gTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGACTGTGTATGAGGCCAAt                                                                  >  2:157494/1‑121 (MQ=255)
                                                                                                        ttGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAAtt                                     >  5:163581/1‑149 (MQ=255)
                                                                                                                      gCTGGGCTTCCAGACCTATGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATTATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATTAGTATTTGTCAATTTGGCAGActttcgt                       <  4:342907/149‑1 (MQ=255)
                                                                                                                       cTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTATGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGAcgttcgtt                      <  6:49877/149‑1 (MQ=255)
                                                                                                                           gCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTAGt                   <  5:163117/148‑1 (MQ=255)
                                                                                                                             ttCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATTAGTATTTGTCAATTGGGCAGACGTTCGTTAGTGc                 >  7:54177/1‑148 (MQ=255)
                                                                                                                             ttCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTAGTGc                 >  7:55923/1‑148 (MQ=255)
                                                                                                                                  gttcTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTTTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTAGTGCAAATgg           <  5:91087/146‑1 (MQ=255)
                                                                                                                                        ggCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTAAATTGGGCAGAAGTTCGTTAGTGCAAATgg           >  6:91087/1‑143 (MQ=255)
                                                                                                                                          caagagTCAAGGTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTAGTGCAAATGGaaa        <  8:1262/142‑1 (MQ=255)
                                                                                                                                          caagagTCAAGGTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTAGTGCAAATGGaaa        >  7:1262/3‑144 (MQ=255)
                                                                                                                                          caagagTCAAGGTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTAGTGCAAATGGAAATTCTa   >  3:55753/3‑149 (MQ=255)
                                                                                                                                           cAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTAGTGCAAATGGAAATTCTAg  <  7:35113/149‑1 (MQ=255)
                                                                                                                                              agTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTa                     >  5:223357/1‑127 (MQ=255)
                                                                                                                                              agTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTa                     <  6:223357/127‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
GTCAGAGCTTTTGCACCTTCTGGACGATCTTCAAGAGCGAGCTTGCCCGATACCACATCCACAAATTCACCAACGACATTCCAGTCGTTCGCTTGGGCCGCACGTTGGATGTAATCACGCTGGGCTTCCAGACCTAGGCCAGAGTCACCTTGACTCTTGGTAGATACTCGAATGTAAGCAACAACGTTTGTCATGATGATGGCTCGTCTGTGTATGAGGCCAATTTATCAACTGCCATGAGTATTTGTCAATTGGGCAGACGTTCGTTAGTGCAAATGGAAATTCTAG  >  NC_002947/1277176‑1277463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑