Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,286,894	A→G	7.0%	T93A (ACC→GCC)	PP_1125 →	putative Helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,286,894	0	A	G	7.0%	162.1 / 2.1	57	T93A (ACC→GCC)	PP_1125	putative Helicase
Reads supporting (aligned to +/- strand):  ref base A (26/27);  new base G (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

CCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTCGACATCCTGTTGCAGGAAGGCCACG  >  NC_002947/1286748‑1287041                                                                                                                                                   |                                                                                                                                                    ccTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCAcc                                                                                                                                                   >  7:101484/1‑149 (MQ=255)          aTGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTg                                                                                                                                          >  6:282341/1‑149 (MQ=255)             aaGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGTGGCCGTGGcgatcacc                                                                                                                                       <  2:347936/149‑8 (MQ=255)             aaGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAg                                                                                                                                       <  4:344318/149‑1 (MQ=255)                 ccgccgCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAAc                                                                                                                                   <  8:101484/149‑1 (MQ=255)                              gAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCaa                                                                                                                      <  2:112184/149‑1 (MQ=255)                              gAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCaa                                                                                                                      <  7:246915/149‑1 (MQ=255)                               aGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAg                                                                                                                     <  5:282341/149‑1 (MQ=255)                                   tGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGTCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGAcc                                                                                                                 >  5:347574/1‑149 (MQ=255)                                   tGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATc                                                                                                                              <  4:16104/136‑1 (MQ=255)                                   tGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATc                                                                                                                              >  3:16104/1‑136 (MQ=255)                                     ccGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTg                                                                                                               >  7:304059/1‑149 (MQ=255)                                          gtggCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGa                                                                                                          >  1:239041/1‑149 (MQ=255)                                               cGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAACCGCCTGGGGATCGCCCCGGCGACCGTGGCCCTGCAGGCACAGATCGTCTTCCAGGACCTGCCCGACCTGa                                                                                                     >  6:302316/1‑149 (MQ=255)                                                gTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGAt                                                                                                    >  1:334175/1‑149 (MQ=255)                                                             ccGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGTCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGc                                                                                       <  6:347574/149‑1 (MQ=255)                                                             ccGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGc                                                                                       <  2:334175/149‑1 (MQ=255)                                                               ggTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCTGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCt                                                                                     <  7:322438/149‑1 (MQ=255)                                                                gTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCt                                                                                     >  6:205364/1‑148 (MQ=255)                                                                    cGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACt                                                                                <  8:304059/149‑1 (MQ=255)                                                                      gCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTc                                                                              <  6:316266/149‑1 (MQ=255)                                                                          gACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCt                                                                          <  6:85029/149‑1 (MQ=255)                                                                          gACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCt                                                                          >  5:85029/1‑149 (MQ=255)                                                                            cGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTc                                                                        <  8:283125/149‑1 (MQ=255)                                                                                  ccTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTg                                                                  >  6:96816/1‑149 (MQ=255)                                                                                       aGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGcc                                                               >  5:170725/1‑147 (MQ=255)                                                                                          cTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAggg                                                          >  4:220810/1‑149 (MQ=255)                                                                                             gCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGcc                                                        <  5:205364/148‑1 (MQ=255)                                                                                              cTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGt                                                      <  3:362229/149‑1 (MQ=255)                                                                                                 ccATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTgg                                                                 <  1:349382/135‑1 (MQ=255)                                                                                                 ccATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTgg                                                                 >  2:349382/1‑135 (MQ=255)                                                                                                 ccATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGc                                                   >  3:30332/1‑149 (MQ=255)                                                                                                  cATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGcc                                                  >  8:121291/1‑149 (MQ=255)                                                                                                  cATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGcc                                                  >  7:85741/1‑149 (MQ=255)                                                                                                    tCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGc                                                >  6:352986/1‑149 (MQ=255)                                                                                                             ccAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGc                                       <  2:239041/149‑1 (MQ=255)                                                                                                                 ggCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAgg                                                           <  5:366266/125‑1 (MQ=255)                                                                                                                 ggCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAgg                                                           >  6:366266/1‑125 (MQ=255)                                                                                                                  gccgccGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCACGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTc                                  <  5:302316/149‑1 (MQ=255)                                                                                                                  gccgccGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCATCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTc                                  <  4:30332/149‑1 (MQ=255)                                                                                                                     gccgGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTcc                                 >  3:17946/1‑147 (MQ=255)                                                                                                                       cgGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGc                             >  1:61626/1‑149 (MQ=255)                                                                                                                         gCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTc                           >  8:274857/1‑149 (MQ=255)                                                                                                                             aCGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTCGACa                       <  7:121291/149‑1 (MQ=255)                                                                                                                               gCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTCGACATc                     <  5:352986/149‑1 (MQ=255)                                                                                                                                ccTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTCGACATcc                    <  5:96816/149‑1 (MQ=255)                                                                                                                                    gTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTcc                                 <  7:45511/132‑1 (MQ=255)                                                                                                                                    gTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTcc                                 >  8:45511/1‑132 (MQ=255)                                                                                                                                      gATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTCGACATCCTGTTg               >  1:75535/1‑148 (MQ=255)                                                                                                                                       aTCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTCGACATCCTGTTGCa             <  6:170725/149‑1 (MQ=255)                                                                                                                                        gggCCACGGTGGCCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTgg                                                    <  6:196651/107‑1 (MQ=255)                                                                                                                                        gggCCACGGTGGCCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTgg                                                    >  5:196651/3‑109 (MQ=255)                                                                                                                                          gCCACGGTGGCCGTGGCCCTGCAGGAACAGATCGTCTTCAACGACCTGCCCGACCTGATGCGCAGCAGCGGACTCAACTTCAGCTTCGACCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGACCAACCACGACCTCCTGATCCAgg           >  8:302368/1‑148 (MQ=255)                                                                                                                                             aCGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTCGACATCCTGTTGCAGGAAgg       <  4:17946/149‑1 (MQ=255)                                                                                                                                                 ccACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTCGACATCCTGTTGCAGGAAGGCCAc   <  2:61626/149‑1 (MQ=255)                                                                                                                                                  cACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGCCCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTCTGCCTGTCCAAGCTCGACATACTGTTGCAGGAAGGCCACg  >  3:273291/1‑149 (MQ=255)                                                                                                                                                  cACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTCGACATCCTGTTGCAGGAAGGCCACg  >  3:304466/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTGTCCAAGCTCGACATCCTGTTGCAGGAAGGCCACG  >  NC_002947/1286748‑1287041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑