Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,389,946 C→G 5.9% A23G (GCC→GGC)  PP_1212 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,389,9460CG 5.9% 224.1 / 3.0 68A23G (GCC→GGC) PP_1212hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (33/31);  new base G (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

TGCGCAGTGCTTGCTAACCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGGCGGCGACAAG  >  NC_002947/1389798‑1390091
                                                                                                                                                    |                                                                                                                                                 
tGCGCAGTGCTTGCTAACCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAgcgc                                                                                                                                                   >  4:8670/1‑149 (MQ=255)
 gcgcAGTGCTTGCTAACCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAgcgcc                                                                                                                                                  >  5:278437/1‑149 (MQ=255)
             ctAACCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATc                                                                                                                                      <  7:20792/149‑1 (MQ=255)
                aCCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGAt                                                                                                                                   >  8:198934/1‑149 (MQ=255)
                aCCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGAt                                                                                                                                   <  5:198191/149‑1 (MQ=255)
                aCCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGACGCTGATCGAt                                                                                                                                   >  4:70890/1‑149 (MQ=255)
                 ccATATATAATCCCGCTTTTTGCTGTCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGAtt                                                                                                                                  >  8:247026/1‑149 (MQ=255)
                          aTCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGGCGCGGCGCTGATc                                                                                                                                      >  1:298942/1‑136 (MQ=255)
                          aTCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGGCGCGGCGCTGATc                                                                                                                                      <  2:298942/136‑1 (MQ=255)
                           tCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTg                                                                                                                        >  8:99599/1‑149 (MQ=255)
                             ccGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGt                                                                                                                       >  5:3383/1‑148 (MQ=255)
                              cGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGt                                                                                                                       >  6:7542/1‑147 (MQ=255)
                              cGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGGCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCa                                                                                                                     <  5:341431/149‑1 (MQ=255)
                                       ctgcCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCgg                                                                                                             <  3:197393/148‑1 (MQ=255)
                                       ctgcCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCgg                                                                                                             <  6:3383/148‑1 (MQ=255)
                                          ccccccGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCAc                                                                                                          <  7:198934/148‑1 (MQ=255)
                                                   agccagcGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAgct                                                                                                <  8:20142/149‑1 (MQ=255)
                                                   agccagcGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAgct                                                                                                <  3:70890/149‑1 (MQ=255)
                                                    gccagcGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAgctg                                                                                               <  5:7542/149‑1 (MQ=255)
                                                     ccagcGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAgctgc                                                                                              >  8:136717/1‑149 (MQ=255)
                                                      cagcGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGgc                                                                                                                  <  2:90171/128‑1 (MQ=255)
                                                      cagcGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGgc                                                                                                                  >  1:90171/1‑128 (MQ=255)
                                                                gTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTc                                                                                                                      <  3:209997/114‑1 (MQ=255)
                                                                gTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTc                                                                                                                      >  4:209997/1‑114 (MQ=255)
                                                                gTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCa                                                                                   <  7:247026/149‑1 (MQ=255)
                                                                 ttGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAg                                                                                  >  2:192958/1‑149 (MQ=255)
                                                                   gACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAgg                                                                                 <  6:138671/148‑1 (MQ=255)
                                                                        gagaCACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCg                                                                           >  8:332840/1‑149 (MQ=255)
                                                                        gagaCACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCg                                                                           >  4:359580/1‑149 (MQ=255)
                                                                           acacCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCt                                                                        <  6:278437/149‑1 (MQ=255)
                                                                           acacCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCt                                                                        >  8:274212/1‑149 (MQ=255)
                                                                               ccATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAgctg                                                                                               <  2:182374/122‑1 (MQ=255)
                                                                               ccATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAgctg                                                                                               >  1:182374/1‑122 (MQ=255)
                                                                                 aTGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGTACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCt                                                                        <  3:155458/143‑1 (MQ=255)
                                                                                 aTGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGTACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCt                                                                        >  4:155458/1‑143 (MQ=255)
                                                                                   gCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGt                                                                 >  6:253318/1‑148 (MQ=255)
                                                                                    ccccTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTaa                                                               >  8:75942/1‑149 (MQ=255)
                                                                                     cccTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTc                                                                            >  2:337290/1‑135 (MQ=255)
                                                                                     cccTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTc                                                                            <  1:337290/135‑1 (MQ=255)
                                                                                     cccTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCt                                                                        <  3:136302/139‑1 (MQ=255)
                                                                                     cccTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCt                                                                        >  4:136302/1‑139 (MQ=255)
                                                                                     cccTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAAc                                                              <  6:244502/149‑1 (MQ=255)
                                                                                          tATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGtt                                                          <  7:74754/148‑1 (MQ=255)
                                                                                          tATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGtt                                                          <  3:8670/148‑1 (MQ=255)
                                                                                           aTGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGGCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTg                                                         <  7:99599/148‑1 (MQ=255)
                                                                                             gACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTa                                                      >  5:291501/1‑149 (MQ=255)
                                                                                             gACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATAGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTa                                                      >  5:252023/1‑149 (MQ=255)
                                                                                              aCTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTAc                                                     >  5:150478/1‑149 (MQ=255)
                                                                                               cTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTAc                                                     >  4:78320/1‑148 (MQ=255)
                                                                                                    aaTGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAAc                                                <  7:136717/148‑1 (MQ=255)
                                                                                                        tgCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGAc                                            <  6:213621/148‑1 (MQ=255)
                                                                                                        tgCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACt                                           <  7:332840/149‑1 (MQ=255)
                                                                                                               tGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGAc                                    >  4:346795/1‑149 (MQ=255)
                                                                                                                 cGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCg                                  <  5:253318/149‑1 (MQ=255)
                                                                                                                     cACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGc                              >  6:364154/1‑149 (MQ=255)
                                                                                                                        cGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACtt                                          <  7:289456/134‑1 (MQ=255)
                                                                                                                        cGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACtt                                          >  8:289456/1‑134 (MQ=255)
                                                                                                                        cGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGa                            >  2:18056/1‑148 (MQ=255)
                                                                                                                        cGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGa                            >  2:243727/1‑148 (MQ=255)
                                                                                                                           aTGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGaaaaa                        <  3:359580/149‑1 (MQ=255)
                                                                                                                                       cAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAg                                                                                  >  6:269406/1‑79 (MQ=255)
                                                                                                                                        agaagaTCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAggc           >  3:262202/1‑149 (MQ=255)
                                                                                                                                         gaagaTCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCa              >  6:51861/1‑145 (MQ=255)
                                                                                                                                         gaagaTCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCa              <  5:51861/145‑1 (MQ=255)
                                                                                                                                           agaTCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAggcggc        <  3:78320/149‑1 (MQ=255)
                                                                                                                                             aTCAGCGGCGCGGCGCTGATCGATTGCCCGGCCTGTCGGGCGCCGGCACTGAAAAAgctg                                                                                               >  2:193811/1‑60 (MQ=38)
                                                                                                                                             aTCAGCGGCGCGGCGCTGATCGATTGCCCGGCCTGTCGGGCGCCGGCACTGAAAAAgctg                                                                                               <  1:193811/60‑1 (MQ=38)
                                                                                                                                               cAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTAAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGGCGGTGAc     >  2:82884/1‑148 (MQ=255)
                                                                                                                                                aGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCATGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGGCGGCGACaa   <  7:75942/149‑1 (MQ=255)
                                                                                                                                                aGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGGCGGCGAc     >  2:163710/1‑147 (MQ=255)
                                                                                                                                                 gcgccgcgccGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGGCGGCGCCAAg  >  7:128690/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGCGCAGTGCTTGCTAACCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGGCGGCGACAAG  >  NC_002947/1389798‑1390091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑