Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,418,249	G→T	5.8%	L236L (CTG→CTT)	purC →	phosphoribosylaminoimidazole‑succinocarboxamide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,418,249	0	G	T	5.8%	267.1 / 2.9	83	L236L (CTG→CTT)	purC	phosphoribosylaminoimidazole‑succinocarboxamide synthase
Reads supporting (aligned to +/- strand):  ref base G (39/39);  new base T (2/3);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTGTACTG  >  NC_002947/1418103‑1418394                                                                                                                                                   |                                                                                                                                                     gTGCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGt                                                                                                                                                     <  2:220770/148‑1 (MQ=255)   gCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑g                                                                                                                                                 >  8:326056/1‑149 (MQ=255)       ggCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGc                                                                                                                                             <  6:116269/149‑1 (MQ=255)        ccgatctgTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGc                                                                                                                                             <  5:70222/141‑1 (MQ=255)           acgaGTTCAGCCCGGACGGCTGCTGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTTACA‑GCGGCACgc                                                                                                                                         <  5:363855/149‑2 (MQ=255)           acgaGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTTACA‑GCGGCACgc                                                                                                                                         <  1:329035/149‑2 (MQ=255)            cgaGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGtt                                                                                                                                        <  5:21506/149‑1 (MQ=255)            cgaGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGtt                                                                                                                                        >  8:269278/1‑149 (MQ=255)             gaGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTTACA‑GCGGCACgccc                                                                                                                                       <  7:155372/149‑4 (MQ=255)             gaGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTc                                                                                                                                       <  5:326167/149‑1 (MQ=255)                ttCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGCAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAC‑GCGGc                                                                                                                                             >  6:70222/1‑140 (MQ=255)                   aGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCaa                                                                                                                                 <  6:2216/149‑1 (MQ=255)                      ccGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAgcg                                                                                                                              >  8:183938/1‑149 (MQ=255)                        ggACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGcc                                                                                                                            <  7:183938/149‑1 (MQ=255)                            ggCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGAt                                                                                                                        <  7:324447/149‑1 (MQ=255)                              cTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATAc                                                                                                                      >  4:297733/1‑149 (MQ=255)                                          ggACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCCCTGTAA‑CCGGCACGGTGACGCAAGCGCCGGAAACCAC‑GCGAttttt                                                                                                         >  1:173599/1‑149 (MQ=255)                                           gACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGAtttttt                                                                                                        >  6:138473/1‑149 (MQ=255)                                            aCAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGAttttttt                                                                                                       <  7:336334/149‑1 (MQ=255)                                              aaGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTg                                                                                                      >  3:271408/1‑148 (MQ=255)                                                ggAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGaa                                                                                                    >  3:63686/1‑148 (MQ=255)                                                ggAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGaa                                                                                                    >  7:66299/1‑148 (MQ=255)                                                        gCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GAtt                                                                                          >  2:86549/1‑149 (MQ=255)                                                         cAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GAttt                                                                                         <  3:297733/149‑1 (MQ=255)                                                         cAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GAttt                                                                                         <  8:66299/149‑1 (MQ=255)                                                          aagaagATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTg                                                                                        <  4:99563/149‑1 (MQ=255)                                                          aagaagATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTg                                                                                        >  1:102665/1‑149 (MQ=255)                                                                  ggACAAGGCCCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTa                                                                                 >  6:378194/1‑148 (MQ=255)                                                                    aCAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GAttt                                                                                         <  7:19468/138‑1 (MQ=255)                                                                    aCAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GAttt                                                                                         >  8:19468/1‑138 (MQ=255)                                                                        ggACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGa                                                                            >  3:228658/1‑147 (MQ=255)                                                                        ggACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAAc                                                                          >  5:203091/1‑149 (MQ=255)                                                                        ggACCGCATCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑TCGGCACCTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGa                                                                            >  5:203097/1‑147 (MQ=255)                                                                           ccGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCCCGGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAG‑GATTTGCGTTTTAAAAGAACGCt                                                                       >  7:221786/1‑149 (MQ=255)                                                                           ccGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCCCAGGAACGCGCCCCGTT‑ACGCCAGGGCCTGGTTAAACGGCAATTTTTTTGAAAAAAA‑‑ATTTGGGGTTTAAaacaacacg                                                                       >  2:173663/1‑146 (MQ=255)                                                                            cGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTg                                                                      <  8:88893/149‑1 (MQ=255)                                                                             gCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTgg                                                                     <  3:106545/149‑1 (MQ=255)                                                                              cTTCCGCCAGGGCCTGGTCGACGTGATCGATGCCTACGCAGAAGTTGCCATGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGt                                                                    <  6:203097/149‑1 (MQ=255)                                                                              cTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGt                                                                    <  6:203091/149‑1 (MQ=255)                                                                               ttCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTa                                                                   >  2:206821/1‑149 (MQ=255)                                                                                 ccGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGAttttttt                                                                                                       <  3:245556/112‑1 (MQ=255)                                                                                 ccGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGAttttttt                                                                                                       >  4:245556/1‑112 (MQ=255)                                                                                   gCCAGGGCGTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCACGCGCCTGATACCAC‑GCGAGTTTTTTGAAAAAAA‑GATTTGCGTTTTTAGAGTCCGCTGGTatggt                                                               >  4:315121/1‑147 (MQ=255)                                                                                   gCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTTGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAC‑GATTTGCGTTTTAAAAGAACGCTGGTatgat                                                               <  5:28303/149‑1 (MQ=255)                                                                                      aGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GAttt                                                                                         >  5:293930/1‑120 (MQ=255)                                                                                      aGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GAttt                                                                                         <  6:293930/120‑1 (MQ=255)                                                                                      aGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATgcg                                                            >  6:113647/1‑149 (MQ=255)                                                                                       gggCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATgcgc                                                           >  5:174722/1‑149 (MQ=255)                                                                                           cTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCa                                                       <  5:138473/149‑1 (MQ=255)                                                                                              ggCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCAtt                                                     <  4:271408/148‑1 (MQ=255)                                                                                              ggCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTg                                                    <  6:174722/149‑1 (MQ=255)                                                                                               gCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTg                                                    >  7:729/1‑148 (MQ=255)                                                                                                   cGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGgagag                                               <  7:326056/149‑1 (MQ=255)                                                                                                      gATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCCCGGTAACGCAAGCGCCGGATACCCC‑GCGATTTTTTTGAAAAAAA‑GATTTTCGGTTTTAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATg                                            >  6:39664/1‑149 (MQ=255)                                                                                                      gATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGGTCACGCAAGCGCCTGATACAAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCGGGGATGATGCGCGGGATTGGAGCGATg                                            >  4:333134/1‑149 (MQ=255)                                                                                                       aTCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAATGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGAt                                             <  1:86549/148‑1 (MQ=255)                                                                                                       aTCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGc                                           >  1:61360/1‑149 (MQ=255)                                                                                                       aTCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGc                                           >  2:274028/1‑149 (MQ=255)                                                                                                            aGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAg                                      <  5:276218/149‑1 (MQ=255)                                                                                                               cTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTgg                                   <  5:39664/149‑1 (MQ=255)                                                                                                                tACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGga                                                  >  4:331858/1‑133 (MQ=255)                                                                                                                tACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGga                                                  <  3:331858/133‑1 (MQ=255)                                                                                                                   gaagaaGTTGCCAAGCGCCTGGGCGTGCCGCGGTAA‑GCGGCCCGTTCACGCAAGCGCCTGGTACCAC‑GCGGTTTTTTTGAAAAAAA‑GATTTGCGGTTTAAAAGGACGCGGGTATGATGCGGGGGATTGGAGAGCTGGCGGAGGGGtg                                 >  8:146641/1‑146 (MQ=255)                                                                                                                    aagaagTTGCCAAGCGCCTGGGCGTGCCGGGGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTGTAACAGAACGCTGGTAAGATGCGCGGGATTGGAGAGATGCCGGAGTGGTCg                                >  6:91131/1‑147 (MQ=255)                                                                                                                      gaagTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACg                            >  7:229371/1‑149 (MQ=255)                                                                                                                          ttGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGAc                        <  1:173663/149‑1 (MQ=255)                                                                                                                           tGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAG‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGAc                        <  8:221786/148‑1 (MQ=255)                                                                                                                             ccAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGa                     >  7:212409/1‑149 (MQ=255)                                                                                                                              cAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACg                                                                         <  5:258188/96‑1 (MQ=255)                                                                                                                              cAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACg                                                                         >  6:258188/1‑96 (MQ=255)                                                                                                                               aaGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTTGAGAGATGCCGGAGTCGTCGACCGGGACGGCtt                   >  6:115396/1‑149 (MQ=255)                                                                                                                                 gcgcCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTTATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCg                 <  4:63686/149‑1 (MQ=255)                                                                                                                                     gtgaacGTGCCGCTTACA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAAt             >  3:213317/6‑149 (MQ=255)                                                                                                                                     cTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAAt             >  1:331038/1‑149 (MQ=255)                                                                                                                                        aacGTGCCGCTTACA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGGAGTGGTGAGATGCCGGAGTGGTCGAAGGGGACGGGGTCGGAATCCg          >  8:142889/3‑149 (MQ=255)                                                                                                                                         gCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGt         <  4:228658/149‑1 (MQ=255)                                                                                                                                         gCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGt         <  5:113647/149‑1 (MQ=255)                                                                                                                                          cGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCTCTGCATTGGTGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGtt        <  5:115396/149‑1 (MQ=255)                                                                                                                                          cGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGtt        <  2:102665/149‑1 (MQ=255)                                                                                                                                          cGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGtt        <  5:378194/149‑1 (MQ=255)                                                                                                                                               cGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTGTACt   >  3:45983/1‑149 (MQ=255)                                                                                                                                               cGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTGTACt   <  1:206821/149‑1 (MQ=255)                                                                                                                                                gCTGTAA‑GCGGCAGGGTCACGCTAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGCTGCCGGAGTGGTCGAACGGGCCGGATTCGAAATCCGTTGTACTg  <  5:91131/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GTGCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAA‑GCGGCACGTTCACGCAAGCGCCTGATACCAC‑GCGATTTTTTTGAAAAAAA‑GATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTGTACTG  >  NC_002947/1418103‑1418394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑