Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,432,880	T→G	7.0%	D320E (GAT→GAG) ‡	xenA →	protein XenA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,432,880	0	T	G	7.0%	212.4 / 6.2	71	D320E (GAT→GAG) ‡	xenA	protein XenA
Reads supporting (aligned to +/- strand):  ref base T (32/34);  new base G (2/3);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

CCGACACCAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCT  >  NC_002947/1432735‑1433026                                                                                                                                                  |                                                                                                                                                    ccGACACCAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTg                                                                                                                                                  <  6:300051/149‑1 (MQ=255) ccGACACCAACATCCCCTGGAGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCtt                                                                                                                                                  <  5:354577/149‑2 (MQ=255)  cGACACCAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTgg                                                                                                                                                 >  1:319396/1‑149 (MQ=255)  cGACACCAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTgg                                                                                                                                                 <  3:224916/149‑1 (MQ=255)      acCAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTc                                                                                                                                             >  2:197206/1‑149 (MQ=255)       ccAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑g                                                                                                                                           <  1:125221/149‑1 (MQ=255)       ccAACATACCGTGGGGTGCTGCGTTCATGGGGCCGTTTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGTCTTTGGTAGGCCGAAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑g                                                                                                                                           <  8:71175/149‑1 (MQ=255)        cAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTGGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGATCCAGCTGGttggcc                                                                                                                                           <  5:105429/149‑5 (MQ=255)        cAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTc                                                                                                                                             <  8:309173/147‑1 (MQ=255)        cAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTc                                                                                                                                             >  7:309173/1‑147 (MQ=255)        cAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑gg                                                                                                                                          >  7:52709/1‑149 (MQ=255)         aaCATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑gg                                                                                                                                          >  2:341598/1‑148 (MQ=255)             tCCCCTGGGGTCCAGCGTTCATAGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAggg                                                                                                                                     >  6:178046/1‑149 (MQ=255)                       tCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGGGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCAcc                                                                                                                           <  3:147528/149‑1 (MQ=255)                         cAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTg                                                                                                                         >  2:260833/1‑149 (MQ=255)                          aGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGGGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGGAGGGCGCGCGCACCTgg                                                                                                                        <  7:169187/149‑1 (MQ=255)                              ttCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGa                                                                                                                    >  5:42296/1‑149 (MQ=255)                                cATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGAc                                                                                                                   <  1:341598/148‑1 (MQ=255)                                 aTGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGAccc                                                                                                                 >  8:164385/1‑149 (MQ=255)                                 aTGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGAccc                                                                                                                 >  5:217355/1‑149 (MQ=255)                                  tGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCg                                                                                                                <  7:164385/149‑1 (MQ=255)                                      gCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGGTTC‑GGTAGGGCGCGCGCACCTGGCCGCCCCGCACt                                                                                                            >  4:285796/1‑149 (MQ=255)                                            tGCCGAGCGCGTGCGCCGGGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCt                                                                                                       >  3:287213/1‑148 (MQ=255)                                              ccGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACTTGGCCGACCCGCACTGGGCTTa                                                                                                     <  1:197206/148‑1 (MQ=255)                                              ccGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGATCCAGCTGGttggc                                                                                                                                            <  1:23127/110‑4 (MQ=255)                                              ccGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGATCCAGCTGGttggc                                                                                                                                            >  2:23127/1‑107 (MQ=255)                                                gAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACtt                                                                                                  <  5:178046/149‑1 (MQ=255)                                                  gcgcGTGCGCCGTGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTtg                                                                                                <  4:287213/149‑1 (MQ=255)                                                   cgcgTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTtgc                                                                                               <  1:260833/149‑1 (MQ=255)                                                     cgTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTtgctg                                                                                             >  5:340601/1‑149 (MQ=255)                                                      gTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGATCCAGCTGGttggc                                                                                                                                            >  2:99324/1‑99 (MQ=255)                                                      gTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGATCCAGCTGGttggc                                                                                                                                            <  1:99324/102‑4 (MQ=255)                                                       tGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGcc                                                                                           <  7:24660/149‑1 (MQ=255)                                                             gcgaagcgaaGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTtgct                                                                                              <  1:216908/140‑1 (MQ=255)                                                             gcgaagcgaaGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTtgct                                                                                              >  2:216908/1‑140 (MQ=255)                                                                aagcgaagcTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGACTTACTTTGCTGCCAAGGAGCTg                                                                                  <  1:122703/149‑1 (MQ=255)                                                                 agcgaagcTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTgg                                                                                 <  5:144218/149‑1 (MQ=255)                                                                   cgaagcTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTgggg                                                                               >  7:214055/1‑149 (MQ=255)                                                                   cgaagcTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGAGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTgggg                                                                               >  7:219727/1‑149 (MQ=255)                                                                            ccGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTg                                                                                                           >  2:202026/1‑112 (MQ=255)                                                                            ccGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTg                                                                                                           <  1:202026/112‑1 (MQ=255)                                                                            ccGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCaa                                                                                         >  4:292418/1‑130 (MQ=255)                                                                            ccGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCaa                                                                                         <  3:292418/130‑1 (MQ=255)                                                                            ccGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGaaaaa                                                                      <  5:180070/149‑1 (MQ=255)                                                                              gTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGc                                                                    <  6:42296/149‑1 (MQ=255)                                                                                   gTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGcctcct                                                               >  4:357915/1‑149 (MQ=255)                                                                                          tGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCtt                                                        >  2:93775/1‑149 (MQ=255)                                                                                           ggggCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTg                                                       >  4:165039/1‑149 (MQ=255)                                                                                                   ggTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGgcgc                                                <  2:319396/148‑1 (MQ=255)                                                                                                     tACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCt                                              >  5:29398/1‑148 (MQ=255)                                                                                                        gccgcAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCGTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATg                                          >  7:172035/1‑149 (MQ=255)                                                                                                                tGGCTGAGGACGCATTGCAGGCCAACCAGCTGGATCTGGGTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTgg                                  <  8:172035/149‑1 (MQ=255)                                                                                                                  gCGGAGGCCGCATTGCATGCCAACCAGCTTGATCTGGTTTC‑GTTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGc                                 <  8:52709/148‑1 (MQ=255)                                                                                                                     gAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGc                                 >  8:189143/1‑145 (MQ=255)                                                                                                                     gAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGc                                 <  7:189143/145‑1 (MQ=255)                                                                                                                     gAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGa                             <  6:340601/149‑1 (MQ=255)                                                                                                                       ggCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGc                           <  6:29398/149‑1 (MQ=255)                                                                                                                        gCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCg                          >  1:49266/1‑149 (MQ=255)                                                                                                                          cGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGt                         >  5:171512/1‑148 (MQ=255)                                                                                                                           gCATTGCAGGCCAACCAGCTGGATCTGGTTTCGGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGG‑AAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTa                       >  1:231424/1‑149 (MQ=255)                                                                                                                              ttGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCAGTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGa                             <  1:143599/140‑1 (MQ=255)                                                                                                                              ttGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGa                             >  2:143599/1‑140 (MQ=255)                                                                                                                                 cAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCt                  >  6:210633/1‑148 (MQ=255)                                                                                                                                  aGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGa                <  3:285796/149‑1 (MQ=255)                                                                                                                                      cAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCAAACCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCt            >  1:123337/1‑149 (MQ=255)                                                                                                                                        aCCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGGGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTg          <  6:217355/149‑1 (MQ=255)                                                                                                                                            gCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAgg      <  2:118720/149‑1 (MQ=255)                                                                                                                                            gCTGGATCTGGTTTC‑GGTAGGGCCCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCCCTGATGCTTGCAgg      >  7:257965/1‑149 (MQ=255)                                                                                                                                             cTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAggg     <  3:357915/149‑1 (MQ=255)                                                                                                                                                gATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGgct  >  8:133025/1‑149 (MQ=255)                                                                                                                                                gATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGgc   <  7:208369/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CCGACACCAACATCCCCTGGGGTCCAGCGTTCATGGGGCCGATTGCCGAGCGCGTGCGCCGCGAAGCGAAGCTGCCCGTGACGTCGGCGTGGGGCTTTGGTACGCCGCAGTTGGCGGAGGCCGCATTGCAGGCCAACCAGCTGGATCTGGTTTC‑GGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCT  >  NC_002947/1432735‑1433026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑