Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,464,196 G→A 9.4% T474T (ACC→ACT) ‡ algX ← alginate biosynthesis protein AlgX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,464,1960GA 9.4% 145.7 / 2.1 52T474T (ACC→ACT) ‡algXalginate biosynthesis protein AlgX
Reads supporting (aligned to +/- strand):  ref base G (30/17);  new base A (2/3);  total (32/20)
Fisher's exact test for biased strand distribution p-value = 3.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

TTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCG  >  NC_002947/1464051‑1464343
                                                                                                                                                   |                                                                                                                                                   
ttGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTc                                                                                                                                                  >  3:12131/1‑149 (MQ=255)
     cTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTgcgc                                                                                                                                             <  7:129098/149‑1 (MQ=255)
     cTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTgcg                                                                                                                                              >  8:129098/1‑148 (MQ=255)
        ttCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAg                                                                                                                                           <  6:81444/148‑1 (MQ=255)
         tCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCAGACCGCGCAgg                                                                                                                                          <  1:347325/148‑1 (MQ=255)
          cGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCa                                                                                                                                            <  4:13256/145‑1 (MQ=255)
          cGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCa                                                                                                                                            >  3:13256/1‑145 (MQ=255)
          cGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCa                                                                                                                                        >  7:204552/1‑149 (MQ=255)
           gATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCAGACCGCGCAGGCag                                                                                                                                       <  3:91652/149‑2 (MQ=255)
             tCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCCCCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTCAAAAGGGTCAGGGCTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACgg                                                                                                                                     >  5:277315/1‑149 (MQ=255)
             tCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACgg                                                                                                                                     >  8:190642/1‑149 (MQ=255)
                 cTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGaa                                                                                                                                 <  3:231430/149‑1 (MQ=255)
                    gtagtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCCGGCACGGCGAAgtt                                                                                                                              >  1:348764/1‑149 (MQ=255)
                    gtagtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAgtt                                                                                                                              >  8:198812/1‑149 (MQ=255)
                    gtagtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCAGACCGCGCAGGCa                                                                                                                                        <  2:151490/139‑1 (MQ=255)
                    gtagtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCAGACCGCGCAGGCa                                                                                                                                        >  1:151490/1‑139 (MQ=255)
                       gtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGt                                                                                                                                                   <  4:139173/125‑1 (MQ=255)
                       gtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGt                                                                                                                                                   >  3:139173/1‑125 (MQ=255)
                            ccTGCGGCGGTACCCGCGGGGGGTGCGGGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGTAATTTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGaa                                                                                                                                 <  3:238845/138‑1 (MQ=255)
                             cTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACATGATGCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGt                                                                                                                       >  1:123845/1‑149 (MQ=255)
                              ggcggggGCACCTGGGGGGCGGGCGGGCAACCACAAAACACGCCCACCGCGCGCGGGGGGGGGCTGTAATTTCGTTTCAAACGGGTCTGGGGCCGCCTCAC‑GG‑GCCTGCCTGGGGGGTCGGCGCAGCCACGGCGAAGTTGTTGCGTTTg                                                                                                                    <  8:204552/148‑1 (MQ=255)
                                cgggggAACCTGCGCGGCGTGCGCGGCACCACCAAACAAGGCCACCGCGACCAGGGCGGGGCTGGAAATTCGTTTTAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTg                                                                                                                    <  7:327698/147‑1 (MQ=255)
                                        ccAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTgg                                                                                                          >  3:285772/1‑149 (MQ=255)
                                        ccAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTgg                                                                                                          >  3:290879/1‑149 (MQ=255)
                                            cgcgGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGCGTCGCCTCAC‑TG‑GCCTGCCCGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTc                                                                                                      <  8:142005/149‑1 (MQ=255)
                                                gcgTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTgc                                                                                                                                               >  4:114819/1‑104 (MQ=255)
                                                gcgTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTgc                                                                                                                                               <  3:114819/104‑1 (MQ=255)
                                                        ggCACCACCAACCACGCCCAACGCTCGCTGGGGGGGGCTGGAAATTCGTTTCAAACGGGCCTTGGGGCGCCTCAG‑GG‑GCCGGCCGGCGCGGGCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGt                                                                                          <  7:198812/149‑1 (MQ=255)
                                                             caccaAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATGAGTTGGAAAAGGGTCATGGGTCGCCTCAA‑TG‑GCATGCCTGGGCGGTCGGCGAGGGCACGGCGATGTTGTTGCGTTTGCAGCGCTTGGCTTGGCCGTTCTGGTTGCCGc                                                                                     >  6:206568/1‑149 (MQ=255)
                                                              accaAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCt                                                                                    >  3:53051/1‑149 (MQ=255)
                                                               ccaAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCTTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttgtt                                                                                                                           <  2:61272/109‑1 (MQ=255)
                                                                caAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTg                                                                                                                    >  3:208563/1‑115 (MQ=255)
                                                                 aaaCAAGGCCAACGCGACCTGGGCGGTGCTGTAATTTGGTTTGAAAAGGGCCATGGGTGGCCTCAC‑TT‑GCGTGCCTGCGGGGTTTGCGATGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTc                                                                                 <  6:64519/149‑1 (MQ=255)
                                                                     aaGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGGTTGAAAAGGGTCAAGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGCCCTTCTGGGTGCCGCTTTCCGGc                                                                             >  5:270134/1‑149 (MQ=255)
                                                                               cgAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTgg                                                                                            >  7:94100/1‑124 (MQ=255)
                                                                                            gCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGa                                                          >  8:264863/1‑145 (MQ=255)
                                                                                            gCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGa                                                          <  7:264863/145‑1 (MQ=255)
                                                                                                gAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTgg                                                                                            >  7:243263/1‑107 (MQ=255)
                                                                                                     tcttttGAAACGGGCCTTGGGGCCCCTCAG‑TG‑GCCTGCCTGCGGGGTCTGCGCTGCCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgctggc                                              <  4:53051/145‑1 (MQ=255)
                                                                                                             aaaGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGt                                     >  6:340060/1‑149 (MQ=255)
                                                                                                               aGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTcc                                   <  7:190642/149‑1 (MQ=255)
                                                                                                                gggTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAgg                                                     >  7:104298/1‑130 (MQ=255)
                                                                                                                   tCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCa                                       >  5:300532/1‑141 (MQ=255)
                                                                                                                     aTGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCt                             >  7:53289/1‑149 (MQ=255)
                                                                                                                         gTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTcgc                         <  5:340060/149‑1 (MQ=255)
                                                                                                                              cacAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTAGCGCATc                    >  3:41665/3‑149 (MQ=255)
                                                                                                                              cTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTccagatgc                    >  8:181769/1‑142 (MQ=255)
                                                                                                                                 aC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTg                  >  6:264534/1‑148 (MQ=255)
                                                                                                                                  c‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTgg                 >  7:287710/1‑148 (MQ=255)
                                                                                                                                        cgtgcctgcGCAGACCGAGCACGCA‑GGCCAAGTTGTTGAGTTTGGAGAGCTTGGATGCGAACTTCTGGGTGCCGCTGTACGGCCCCTGTACCGCGACCGCCCGCCGGCGCTGGCAGGCCCAGTCCTCGTCTTCGCGCATCTGGatgcc            >  8:264074/3‑144 (MQ=255)
                                                                                                                                         ctgcctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCAGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGAc            >  5:204243/1‑148 (MQ=255)
                                                                                                                                          tgcctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACg           >  3:107997/1‑148 (MQ=255)
                                                                                                                                                cgcgGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGc    >  4:283549/1‑149 (MQ=255)
                                                                                                                                                  cgGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCg  <  8:287710/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCAC‑TG‑GCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCG  >  NC_002947/1464051‑1464343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑