Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 188,618 G→A 6.0% G243R (GGG→AGG)  PP_0165 → GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947188,6180GA 6.0% 248.5 / 2.7 82G243R (GGG→AGG) PP_0165GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (41/35);  new base A (2/3);  total (44/38)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

CAGCCTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTT  >  NC_002947/188470‑188766
                                                                                                                                                    |                                                                                                                                                    
cAGCCTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCg                                                                                                                                                      >  1:331092/1‑149 (MQ=255)
 aGCCTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCgg                                                                                                                                                     <  4:260737/149‑1 (MQ=255)
   ccTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGc                                                                                                                                                   >  6:139222/1‑149 (MQ=255)
    cTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCt                                                                                                                                                  <  2:219600/149‑1 (MQ=255)
       ccAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGt                                                                                                                                                <  1:130267/148‑1 (MQ=255)
       ccAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCCGGTCAGGCTGTc                                                                                                                                               <  7:162111/149‑1 (MQ=255)
        cAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCAGACCGGGCTGTc                                                                                                                                               >  4:69742/1‑148 (MQ=255)
         agagTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCCGGTCAGGCTGTcct                                                                                                                                             <  2:40395/149‑3 (MQ=255)
         agagTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCCGGTCAGGCTGTcct                                                                                                                                             <  6:316563/149‑3 (MQ=255)
         agagTTGCCACGCACGCCGGAGCTAAGCCGCGTAGTGCAAGCGATGAACCAGATGGTCCAGAAACTCAAAGACCTGTACACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCATACCAGGACAGCCTGACCTGGCTGTCg                                                                                                                                              >  2:130260/1‑148 (MQ=255)
                ccACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAAccgcc                                                                                                                                       <  6:243507/148‑1 (MQ=255)
                 cacgcacgCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACcgccgc                                                                                                                                     >  7:305333/1‑149 (MQ=255)
                  acgcacgcCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCgg                                                                                                                                                     >  6:121695/1‑132 (MQ=255)
                  acgcacgcCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCgg                                                                                                                                                     <  5:121695/132‑1 (MQ=255)
                       cgcCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTAtttt                                                                                                                               <  7:185225/149‑1 (MQ=255)
                       cgcCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGGTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTAtttt                                                                                                                               <  4:5274/149‑1 (MQ=255)
                        gcCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTAtttt                                                                                                                               >  4:358487/1‑148 (MQ=255)
                        gcCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTAtttt                                                                                                                               >  1:255785/1‑148 (MQ=255)
                          cGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGaa                                                                                                                            <  5:261952/149‑1 (MQ=255)
                            gAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCCCTATTTTGAAAt                                                                                                                          >  7:213399/1‑149 (MQ=255)
                               cTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATg                                                                                                                         >  3:243326/1‑147 (MQ=255)
                               cTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCa                                                                                                                       <  3:69742/149‑1 (MQ=255)
                               cTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCa                                                                                                                       <  3:235538/149‑1 (MQ=255)
                                     cgcgTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGaa                                                                                                                 <  3:207276/149‑1 (MQ=255)
                                            tGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCgg                                                                                                          <  5:139222/149‑1 (MQ=255)
                                            tGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCCGGTCAGGCTGTcctg                                                                                                                                            >  2:320519/1‑112 (MQ=255)
                                            tGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGCACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCgg                                                                                                          <  3:104094/149‑1 (MQ=255)
                                              cAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCggg                                                                                                         >  1:365368/1‑148 (MQ=255)
                                              cAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCggg                                                                                                         >  7:136162/1‑148 (MQ=255)
                                               aaGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGt                                                                                                        >  1:353344/1‑148 (MQ=255)
                                                aGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTaa                                                                                                      <  7:124857/149‑1 (MQ=255)
                                                     tGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAAc                                                                                                 <  4:191229/149‑1 (MQ=255)
                                                       aaCCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAAcc                                                                                                <  4:243326/148‑1 (MQ=255)
                                                        aCCAGATGGTAGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCTCAGTTAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCTCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTg                                                                                              <  1:130260/149‑1 (MQ=255)
                                                          cAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTgg                                                                                             >  8:81066/1‑148 (MQ=255)
                                                            gATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTgg                                                                                             >  6:264117/1‑146 (MQ=255)
                                                            gATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTgg                                                                                             >  5:1286/1‑146 (MQ=255)
                                                             aTGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCgg                                                                                                          >  6:226034/1‑132 (MQ=255)
                                                             aTGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCgg                                                                                                          <  5:226034/132‑1 (MQ=255)
                                                                   gagaAACTCAAAGCCCTGTTCACCGAGCCGGCCGAGCGCATTGAGCGGCTCCGTGCGGAGTCCGACCAGGACAGCCTGACCGGGCTGTCGACGCGCCGCTATTTTGAAATGCAGCTGAACAAACGGGTAAGCAACCTGGAAGCcgcgcg                                                                                   >  2:261242/1‑149 (MQ=255)
                                                                   gagaAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGAcgcgcg                                                                                   >  2:100529/1‑149 (MQ=255)
                                                                     gaAACTCAAAGCCCTGTTCACGGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCTAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGtta                                                                                                      <  6:109589/128‑3 (MQ=255)
                                                                     gaAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGAcgcgcgcg                                                                                 >  5:271867/1‑149 (MQ=255)
                                                                     gaAACTCAAAGCCCAGTTCAACGAGCAGGACGAGCGCAGTGAGCCGCTGCGTGCGGAGTCCTACCAGGACAGACTGACCGCGCTGTCGAACCGCCGCTATTTTGAAATGCAGATGAACAATCCGCTaa                                                                                                      >  5:109589/1‑128 (MQ=255)
                                                                                cttcattCACCGAGCAGGCCTAGCCCAGGGAGCTGCTGCGTGCGGAGTCCTACCAGGACAGCCTGAGCGGGCTGTGGAACCGACGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACctg                                                                      <  7:247958/144‑1 (MQ=255)
                                                                                cccTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACctg                                                                      <  7:81066/149‑1 (MQ=255)
                                                                                 ccTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACctgc                                                                     >  4:133585/1‑149 (MQ=255)
                                                                                  cTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATc                                                                                                            >  2:133210/1‑109 (MQ=255)
                                                                                  cTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATc                                                                                                            <  1:133210/109‑1 (MQ=255)
                                                                                  cTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACctgct                                                                    >  3:349233/1‑149 (MQ=255)
                                                                                    gTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGt                                                                  >  1:58715/1‑149 (MQ=255)
                                                                                       cACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACgcgcgcgc                                                                                <  2:7395/132‑1 (MQ=255)
                                                                                       cACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACgcgcgcgc                                                                                >  1:7395/1‑132 (MQ=255)
                                                                                       cACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTtgc                                                               >  4:167607/1‑149 (MQ=255)
                                                                                       cACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTtgc                                                               >  1:183500/1‑149 (MQ=255)
                                                                                        aCCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTtgct                                                              >  7:304390/1‑149 (MQ=255)
                                                                                          cGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTtgctgc                                                            <  1:100529/149‑1 (MQ=255)
                                                                                            aGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGCACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCgg                                                          <  8:213399/149‑1 (MQ=255)
                                                                                                ggCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTc                                                      <  3:167607/149‑1 (MQ=255)
                                                                                                 gCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGtt                                                       >  2:301823/1‑147 (MQ=255)
                                                                                                   cGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGTAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGGGCCGGTTACCTGCTGTTGCTGCGGGTTCAgg                                                   >  8:35675/1‑149 (MQ=255)
                                                                                                   cGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAgg                                                   <  2:353344/149‑1 (MQ=255)
                                                                                                    gAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAggg                                                  <  2:365368/149‑1 (MQ=255)
                                                                                                      gcgcAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGcc                                                >  3:288855/1‑149 (MQ=255)
                                                                                                       cgcAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCct                                               <  3:358487/149‑1 (MQ=255)
                                                                                                         cAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCctgg                                             <  6:1286/149‑1 (MQ=255)
                                                                                                              aGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCtggctggc                                        <  2:183500/149‑1 (MQ=255)
                                                                                                                   cTGCGTGCGGAGTCCTACCAGGACAGCCTGCCCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGaa                                   >  5:40397/1‑149 (MQ=255)
                                                                                                                       gtgcgGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAAcgcc                               >  2:156225/1‑149 (MQ=255)
                                                                                                                        tgcgGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAAcgccc                              <  6:271867/149‑1 (MQ=255)
                                                                                                                              gTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTGCAGGGCCTGGCTGGCCTGAACGCCCGCCTTg                        <  8:305333/149‑1 (MQ=255)
                                                                                                                                 cTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTTAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAAGGCCCGCCTTggc                      <  2:255785/148‑1 (MQ=255)
                                                                                                                                 cTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcg                     >  4:188258/1‑149 (MQ=255)
                                                                                                                                 cTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcg                     >  7:83157/1‑149 (MQ=255)
                                                                                                                                  tACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcgg                    >  6:117848/1‑149 (MQ=255)
                                                                                                                                  tACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcgg                    >  5:240325/1‑149 (MQ=255)
                                                                                                                                   ggttcgACAGCCCGGTCAGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcggc                   >  2:340866/6‑149 (MQ=255)
                                                                                                                                    ccAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcggc                   >  6:168521/1‑148 (MQ=255)
                                                                                                                                    ccAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcggc                   >  7:202764/1‑148 (MQ=255)
                                                                                                                                    ccAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCACTCCTTGGCGGcc                  >  6:291426/1‑149 (MQ=255)
                                                                                                                                     cAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCa                 <  4:288855/149‑1 (MQ=255)
                                                                                                                                          cAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTa            <  2:58715/149‑1 (MQ=255)
                                                                                                                                              cTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCgc        >  8:92420/1‑148 (MQ=255)
                                                                                                                                                gACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGAc       >  6:310847/1‑148 (MQ=255)
                                                                                                                                                 aCCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGAcc      <  1:156225/148‑1 (MQ=255)
                                                                                                                                                   cGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAgt   >  4:219531/1‑149 (MQ=255)
                                                                                                                                                    gggCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAgtt  <  3:133585/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGCCTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTT  >  NC_002947/188470‑188766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑