Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,477,382 G→A 9.7% K12K (AAG→AAA)  PP_1291 → PhoH family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,477,3820GA 9.7% 108.2 / 3.0 42K12K (AAG→AAA) PP_1291PhoH family protein
Reads supporting (aligned to +/- strand):  ref base G (19/18);  new base A (2/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

AGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTGGAGGAACTCGACAAGCTCAAGACCGGCAAACAGAGCATCGC  >  NC_002947/1477241‑1477522
                                                                                                                                             |                                                                                                                                            
aGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATcc                                                                                                                                       >  2:170987/1‑149 (MQ=255)
    aGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTa                                                                                                                                   <  1:170987/149‑1 (MQ=255)
    aGGTTCGCCTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTCTGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTa                                                                                                                                   <  4:24144/149‑1 (MQ=255)
       ttCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTAtgt                                                                                                                                >  5:322356/1‑149 (MQ=255)
         cGCTTCCCGCCCGTAACAACGCGGATACGGCGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGc                                                                                                                              >  1:34714/1‑149 (MQ=255)
          gCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGc                                                                                                                              <  8:2474/148‑1 (MQ=255)
          gCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCt                                                                                                                             >  4:178559/1‑149 (MQ=255)
            ttCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTc                                                                                                                            >  6:306978/1‑148 (MQ=255)
               ccgcccgTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATa                                                                                                                        >  4:49627/1‑149 (MQ=255)
                cgcccgTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATAc                                                                                                                       >  6:265299/1‑149 (MQ=255)
                       aacaacGCGGATACGGGGACAGCGGCAGTCACTCTGTGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTc                                                                                                                >  3:353102/1‑149 (MQ=255)
                       aacaacGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTc                                                                                                                >  4:261196/1‑149 (MQ=255)
                        acaacGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTc                                                                                                                <  5:306978/148‑1 (MQ=255)
                                  tACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGa                                                                                                     <  3:32436/149‑1 (MQ=255)
                                           aGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAACCAATCCTGTATGTGCTCGATACCAACGTCCTGATCCACGACCCTAAcgc                                                                                            >  2:36771/1‑149 (MQ=255)
                                           aGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAACCAATCCTGTATGTGCTCGATACCAACGTCCTGATCCACGACCCTAAcgc                                                                                            >  2:31145/1‑149 (MQ=255)
                                              ggCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGtt                                                                                         <  7:131725/149‑1 (MQ=255)
                                               gCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTa                                                                                        <  5:265299/149‑1 (MQ=255)
                                               gCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAACAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGtt                                                                                         >  6:336073/1‑148 (MQ=255)
                                                       tctGCGGCGTAACAGACCACGCCGTGTGGCTTCGGCATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACtt                                                                                >  1:67827/1‑149 (MQ=255)
                                                           ctgcgTAACAGACCACTCCGTGTGGCTTCGACATAAGGTGACCGAGTATGTATGACCAACGACGCAACCCTTCCTCCAGCAAGCCATTCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCga                                                                             <  1:243606/146‑1 (MQ=255)
                                                           cggcgTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTc                                                                               <  3:261196/146‑1 (MQ=255)
                                                           cggcgTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCgag                                                                            <  3:49627/149‑1 (MQ=255)
                                                             gcgTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAAc                                                                                  <  7:85334/141‑1 (MQ=255)
                                                             gcgTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAAc                                                                                  >  8:85334/1‑141 (MQ=255)
                                                                tAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCa                                                                       <  2:34714/149‑1 (MQ=255)
                                                                  aCAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCAcc                                                                     >  4:39348/1‑149 (MQ=255)
                                                                                    cTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGa                                                   >  8:157577/1‑149 (MQ=255)
                                                                                     ttCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACAAACGTCCTGATTCACGACCCTAACTCGTTACTGAACTTCGAGGAGAACAATGTCGCAATCCCGATGAc                                                  >  6:116998/1‑149 (MQ=255)
                                                                                        gACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAACAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACgg                                                <  5:336073/148‑1 (MQ=255)
                                                                                              aGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAACCAATCCTGTATGTGCTCGATACCAACGTCCTGATCCACGACCCTAACGCGTTACTGAACTTCGAGGAGCNCCATGTCGCCATCCCGATGACGGTGCTgga                                         <  1:31145/149‑1 (MQ=255)
                                                                                              aGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAACCAATCCTGTATGTGCTCGATACCAACGTCCTGATCCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTgga                                         <  1:36771/149‑1 (MQ=255)
                                                                                                     cGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTGGAGGAACTc                                  <  6:322356/149‑1 (MQ=255)
                                                                                                           tGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTGGAGGAACTCGACaa                             <  3:39348/148‑1 (MQ=255)
                                                                                                               tGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTGGAGGAACTCGACAAGCTCa                        <  3:178559/149‑1 (MQ=255)
                                                                                                                    aaGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTGGAGGACCTCGACAAGCTCAAGAcc                   >  8:245886/1‑149 (MQ=255)
                                                                                                                          ggaaCCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTg                                              >  3:65255/3‑116 (MQ=255)
                                                                                                                          gCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTg                                              <  4:65255/116‑1 (MQ=255)
                                                                                                                              cccTTCCTCCAGCAATCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTGGAGGAACTCGACAAGCTCAAGACCGGCAAAgaga         <  5:116998/149‑1 (MQ=255)
                                                                                                                                 ttCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTggag                                        <  7:169811/115‑1 (MQ=255)
                                                                                                                                 ttCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTggag                                        >  8:169811/1‑115 (MQ=255)
                                                                                                                                     tccAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGCCCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTGGAGGAACTCGACAAGCTCAAGACCGGCAAACAGAGCATcgc  >  2:365334/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                            
AGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGACCAAGGACGCAACCCTTCCTCCAGCAAGCCAATCCTGTATGTGCTCGATACCAACGTCCTGATTCACGACCCTAACGCGTTACTGAACTTCGAGGAGCACCATGTCGCCATCCCGATGACGGTGCTGGAGGAACTCGACAAGCTCAAGACCGGCAAACAGAGCATCGC  >  NC_002947/1477241‑1477522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑