| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,544,499 | C→G | 20.1% | intergenic (+58/+56) | mdfA → / ← ampG | multidrug MFS transporter/muropeptide permease AmpG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,544,499 | 0 | C | G | 20.1% | 53.6 / 12.1 | 30 | intergenic (+58/+56) | mdfA/ampG | multidrug MFS transporter/muropeptide permease AmpG |
| Reads supporting (aligned to +/- strand): ref base C (12/12); new base G (3/3); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01 | |||||||||||
CCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGT‑GGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAG > NC_002947/1544351‑1544646 | ccTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGT‑GGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGGAGCTAGATAATCGTTATTGCCTGGGCCGG‑CCTATTATCGGGTAAACCCGCTCCTACAAAGGgccgcgc > 2:257657/1‑149 (MQ=255) gggggggggggggggCGGTGGCGGTGGGGGCGGTG‑GGTGTGTGGGGGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTgtgt < 4:208076/148‑1 (MQ=18) gggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGT‑GGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTATTCGCGGGTAAACCCCCTACGACAACGGGCCGCGCCGCGTTg > 6:99623/1‑147 (MQ=255) ggtgggcCGTTGGCGCTGTGGCGGCTGTGGTTTGT‑GGTGGGTGGGCGGCCGGGGG‑AAGGGTCTGTAGCTCGATAATCGGTTTTGCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCgg < 5:133614/146‑1 (MQ=38) gTTGGCGCTGTGGCCGCTGTGGTTTGT‑GGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTAccc > 1:83120/1‑149 (MQ=255) ttGGCGCTGTGGCCGCTGTGGTTTGT‑GGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCcg < 2:83120/149‑1 (MQ=255) ttGGCGCTGTGGCCGCTGTGGTTTGT‑GGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCGCTTCGCGGGTAAAACCCCGCCGACACACGGCCGCGCCGCGTTGTTGGGGGGGGgtttgccg > 4:323491/1‑144 (MQ=255) tgtgGGTTGG‑GGGGGGGGGGCCGGCTGGGGCACGGGGCTGTCGCCTGAAAATGGGTA‑TGCCGGGGGCGGCCCTCTTCGCGGCTAACCCCGCCCAAACCCCGGGCCGCGCCGTTTTGTTGCCGGGGTTTAACCCGCGAAGAGGCCAGcac < 4:202719/149‑1 (MQ=255) gTTTGT‑GGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCACCGACACAGGGCCGCGCCGCGTTGTAGGAGCGGGGTTGCCCGCGCAGAGGGCAGccccggg > 6:353596/1‑142 (MQ=37) tttGT‑GGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAgg < 2:267657/146‑1 (MQ=255) tttGT‑GGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCAGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAgg > 1:267657/1‑146 (MQ=255) t‑ggtggGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAAAACCGCTGCGACACAGGGCCGCGCCGCGTTGTTGGGGCGGGGTTACCCGCGCAGAGGCCCGCACAGGCAATaa > 7:15195/1‑147 (MQ=38) gcggcgTGGGT‑AAGGGGCTGTCGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAACCCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAgg < 3:54330/149‑1 (MQ=255) tAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTgg < 5:353596/149‑1 (MQ=255) aGCTCGATCATCGGTATTGCCGGGGCCGG‑CCTATTCGCGTGTAAACCCGCTCCTACACAGGGCCGAGCCGCTTTGTAGGAGCGGTTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTgg < 1:257657/148‑1 (MQ=38) gATAATCGGTATTCCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGCGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTcc < 3:323491/149‑1 (MQ=255) ttCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTc < 7:23240/147‑1 (MQ=255) tCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTAAGCCCCCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTg > 7:185596/1‑149 (MQ=255) ttaCCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCAc > 3:95703/3‑148 (MQ=255) gCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAggg > 7:264732/1‑149 (MQ=255) gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGa < 3:182258/136‑1 (MQ=25) gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGa > 4:182258/1‑136 (MQ=25) tCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg > 4:110018/1‑145 (MQ=255) tCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg < 3:110018/145‑1 (MQ=255) cTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga < 5:99623/148‑1 (MQ=255) cTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgat > 2:98178/1‑149 (MQ=37) tACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGAt > 1:213147/1‑132 (MQ=25) tACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGAt < 2:213147/132‑1 (MQ=25) aaaacaGGGCCGCGCCGGTTTGTTGGTGCGGGTTTACCCGCGAATAGGCCATCACAGGCAATAAAAAGCTTCAGGGCCGCTCAGCCACCGAGTTGGCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc < 8:15195/146‑1 (MQ=255) acacAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgat > 3:291698/1‑147 (MQ=255) cacaGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGAAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcag > 8:203265/1‑149 (MQ=255) ccgcgccgcTTTGTAGGAGCGGTTTAACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGc < 3:26705/149‑1 (MQ=255) ccgcgccgcTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGc < 8:264732/149‑1 (MQ=255) gccgcTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGGAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAg > 1:260033/1‑149 (MQ=255) gccgcTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAg > 5:298084/1‑149 (MQ=255) | CCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGT‑GGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGG‑CCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAG > NC_002947/1544351‑1544646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |