Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,568,909 C→T 7.9% intergenic (+46/‑281) pcaK → / → pcaF‑I 4‑hydroxybenzoate transporter/beta‑ketoadipyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,568,9090CT 7.9% 180.9 / 3.4 63intergenic (+46/‑281)pcaK/pcaF‑I4‑hydroxybenzoate transporter/beta‑ketoadipyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base C (29/29);  new base T (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01

GAGCTTCGAGCAGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTACC  >  NC_002947/1568764‑1569055
                                                                                                                                                 |                                                                                                                                                   
gAGCTTCGAGCAGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCg                                                                                                                                                    >  4:145110/1‑147 (MQ=255)
          cAGGTGCTGAGGGCGTTGCTGGTACCGGCGGCGCTGGGGACCGTGGGAGTGGTGGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCACATTCACAGCGTAACGCCGCCCCTGCGGGAgcggcg                                                                                                                                         <  6:371113/148‑1 (MQ=255)
           aGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGtt                                                                                                                                       >  1:283682/1‑149 (MQ=255)
           aGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGtt                                                                                                                                       <  6:245955/149‑1 (MQ=255)
             gTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTtgt                                                                                                                                     >  8:290677/1‑149 (MQ=255)
              tGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTtgtg                                                                                                                                    <  7:257086/149‑1 (MQ=255)
                cTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCCCCTGCGGGAGCGGCGttacgct                                                                                                                                  <  5:287745/149‑6 (MQ=255)
                 tGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGtgctg                                                                                                                                 >  6:102067/1‑149 (MQ=255)
                    cGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAAGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCa                                                                                                                               >  2:169286/1‑148 (MQ=255)
                     ggCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAAt                                                                                                                             >  8:177170/1‑149 (MQ=255)
                      gCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAAt                                                                                                                             >  4:90705/1‑148 (MQ=255)
                      gCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATc                                                                                                                            <  6:342078/149‑1 (MQ=255)
                           gCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGgc                                                                                                                       >  3:300745/1‑149 (MQ=255)
                            cTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGgc                                                                                                                       <  2:20246/148‑1 (MQ=255)
                            cTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGgc                                                                                                                       <  2:283682/148‑1 (MQ=255)
                                   cggcggCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGtt                                                                                                               <  6:68071/149‑1 (MQ=255)
                                     gcggcgCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCACACAGTTGc                                                                                                             <  3:145110/149‑1 (MQ=255)
                                              gCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑Gt                                                                                                   >  4:135814/1‑149 (MQ=255)
                                                gACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCACAACGCCGCCCCTGCGGGAGCGGCGttacgc                                                                                                                                   >  8:110924/1‑112 (MQ=37)
                                                gACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCACAACGCCGCCCCTGCGGGAGCGGCGttacgc                                                                                                                                   <  7:110924/116‑5 (MQ=37)
                                                gACCGGGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTgg                                                                                                 <  6:368426/149‑1 (MQ=255)
                                                   cGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGt                                                                                              <  3:61994/149‑1 (MQ=255)
                                                   cGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGt                                                                                              <  7:147519/149‑1 (MQ=255)
                                                    gTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTg                                                                                             >  8:27755/1‑149 (MQ=255)
                                                      gggAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGa                                                                                           >  8:14758/1‑149 (MQ=255)
                                                             gtcgtcAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGt                                                                                    <  2:113038/149‑1 (MQ=255)
                                                             gtcgtcAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGt                                                                                    >  5:313882/1‑149 (MQ=255)
                                                             gtcgtcAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATCCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGt                                                                                    >  4:367320/1‑149 (MQ=255)
                                                                  cAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGcaa                                                                                >  8:148239/1‑148 (MQ=255)
                                                                            gTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGtt                                                                       <  5:102067/147‑1 (MQ=255)
                                                                            gTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGtt                                                                       <  3:345445/147‑1 (MQ=255)
                                                                            gTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTc                                                                      <  1:169286/148‑1 (MQ=255)
                                                                                  ccagCCGACGCAAACTGAGGGCGAGGTCCGCGTTGCGGCCCAGTCAAAGCGTAAAGCCGCTACCGCAGGGGCGGCGTTGTGCTGCCATAAGGGCGCACAGTTGCACTGCGC‑GTGGTGTGGATAGTCGGGCAACAAATGTTTCGTTAATc                                                               >  8:183226/4‑149 (MQ=255)
                                                                                  cACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTaa                                                                 >  2:102819/1‑147 (MQ=255)
                                                                                   aCGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCg                                                              >  2:103715/1‑149 (MQ=255)
                                                                                   aCGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCg                                                              <  4:300745/149‑1 (MQ=255)
                                                                                      ccGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGcac                                                           <  1:103715/149‑1 (MQ=255)
                                                                                         aCGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGa                                                        <  6:313882/149‑1 (MQ=255)
                                                                                             aaCCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGt                                                    >  5:191853/1‑149 (MQ=255)
                                                                                              aCCTGAGGTCGAGGGCCGCGTTGCGGACCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCTTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GGGGGGGGGATAGGCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTc                                                   <  7:183226/149‑1 (MQ=255)
                                                                                              aCCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTc                                                   <  6:333849/149‑1 (MQ=255)
                                                                                                cTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGa                                                 <  6:191853/149‑1 (MQ=255)
                                                                                                 tGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGAt                                                >  7:121697/1‑149 (MQ=255)
                                                                                                      gaatgggcCGCAACGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCg                                           >  2:162890/5‑149 (MQ=255)
                                                                                                           ggcCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTg                                      >  5:36701/1‑149 (MQ=255)
                                                                                                              cgcgTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGt                                     >  7:229403/1‑147 (MQ=255)
                                                                                                               gcgTTGCGGCCCATCCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAg                                  <  3:367320/149‑1 (MQ=255)
                                                                                                                 gTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGt                                <  6:36701/149‑1 (MQ=255)
                                                                                                                      ggCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGtaccta                           <  3:90705/149‑1 (MQ=255)
                                                                                                                       gCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGAtt                                       >  2:37712/1‑136 (MQ=255)
                                                                                                                       gCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGAtt                                       <  1:37712/136‑1 (MQ=255)
                                                                                                                       gCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGtacctac                          >  1:370137/1‑149 (MQ=255)
                                                                                                                        cccATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGAt                                                >  3:341640/1‑126 (MQ=255)
                                                                                                                        cccATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGAt                                                <  4:341640/126‑1 (MQ=255)
                                                                                                                         ccATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCa                        <  7:290677/149‑1 (MQ=255)
                                                                                                                                caGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCt                 <  3:135814/149‑1 (MQ=255)
                                                                                                                                 aGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAgg                                 <  5:227313/132‑1 (MQ=255)
                                                                                                                                 aGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAgg                                 >  6:227313/1‑132 (MQ=255)
                                                                                                                                   cacaaCGCCGCCCCTGCGGGAGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTaa              >  7:33888/4‑149 (MQ=255)
                                                                                                                                      aaCGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCt           >  3:73284/1‑149 (MQ=255)
                                                                                                                                         gccgcTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTAAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTggg        <  8:229403/149‑1 (MQ=255)
                                                                                                                                         gacgcTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGGGGATAGGCGTGCAACAAATGGTTCGTTACTAGCACAGATAGTCTAGTATCGGATTGTAAGGTCACTACCAGTCTCCTTAATATggg        >  1:112234/3‑149 (MQ=255)
                                                                                                                                            gcTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTCAGGTACCTACCAGTCTCCTTAATCTGGGtg      >  7:129505/1‑147 (MQ=255)
                                                                                                                                                 cGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGCAGTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTAcc  >  1:87252/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GAGCTTCGAGCAGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTACC  >  NC_002947/1568764‑1569055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑