Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,568,912 A→G 7.6% intergenic (+49/‑278) pcaK → / → pcaF‑I 4‑hydroxybenzoate transporter/beta‑ketoadipyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,568,9120AG 7.6% 192.1 / 4.8 66intergenic (+49/‑278)pcaK/pcaF‑I4‑hydroxybenzoate transporter/beta‑ketoadipyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base G (2/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

CAGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTACCGAA  >  NC_002947/1568774‑1569058
                                                                                                                                          |                                                                                                                                                   
cAGGTGCTGAGGGCGTTGCTGGTACCGGCGGCGCTGGGGACCGTGGGAGTGGTGGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCACATTCACAGCGTAACGCCGCCCCTGCGGGAgcggcg                                                                                                                                            <  6:371113/148‑1 (MQ=255)
 aGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGtt                                                                                                                                          >  1:283682/1‑149 (MQ=255)
 aGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGtt                                                                                                                                          <  6:245955/149‑1 (MQ=255)
   gTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTtgt                                                                                                                                        >  8:290677/1‑149 (MQ=255)
    tGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTtgtg                                                                                                                                       <  7:257086/149‑1 (MQ=255)
      cTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCCCCTGCGGGAGCGGCGttacgct                                                                                                                                     <  5:287745/149‑6 (MQ=255)
       tGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGtgctg                                                                                                                                    >  6:102067/1‑149 (MQ=255)
          cGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAAGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCa                                                                                                                                  >  2:169286/1‑148 (MQ=255)
           ggCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAAt                                                                                                                                >  8:177170/1‑149 (MQ=255)
            gCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAAt                                                                                                                                >  4:90705/1‑148 (MQ=255)
            gCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATc                                                                                                                               <  6:342078/149‑1 (MQ=255)
                 gCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGgc                                                                                                                          >  3:300745/1‑149 (MQ=255)
                  cTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGgc                                                                                                                          <  2:20246/148‑1 (MQ=255)
                  cTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGgc                                                                                                                          <  2:283682/148‑1 (MQ=255)
                         cggcggCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGtt                                                                                                                  <  6:68071/149‑1 (MQ=255)
                           gcggcgCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCACACAGTTGc                                                                                                                <  3:145110/149‑1 (MQ=255)
                                    gCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑Gt                                                                                                      >  4:135814/1‑149 (MQ=255)
                                      gACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCACAACGCCGCCCCTGCGGGAGCGGCGttacgc                                                                                                                                      >  8:110924/1‑112 (MQ=37)
                                      gACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCACAACGCCGCCCCTGCGGGAGCGGCGttacgc                                                                                                                                      <  7:110924/116‑5 (MQ=37)
                                      gACCGGGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTgg                                                                                                    <  6:368426/149‑1 (MQ=255)
                                         cGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGt                                                                                                 <  7:147519/149‑1 (MQ=255)
                                         cGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGt                                                                                                 <  3:61994/149‑1 (MQ=255)
                                          gTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTg                                                                                                >  8:27755/1‑149 (MQ=255)
                                            gggAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGa                                                                                              >  8:14758/1‑149 (MQ=255)
                                                   gtcgtcAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGt                                                                                       >  5:313882/1‑149 (MQ=255)
                                                   gtcgtcAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGt                                                                                       <  2:113038/149‑1 (MQ=255)
                                                   gtcgtcAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATCCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGt                                                                                       >  4:367320/1‑149 (MQ=255)
                                                        cAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGcaa                                                                                   >  8:148239/1‑148 (MQ=255)
                                                                  gTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGtt                                                                          <  5:102067/147‑1 (MQ=255)
                                                                  gTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGtt                                                                          <  3:345445/147‑1 (MQ=255)
                                                                  gTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTc                                                                         <  1:169286/148‑1 (MQ=255)
                                                                        ccagCCGACGCAAACTGAGGGCGAGGTCCGCGTTGCGGCCCAGTCAAAGCGTAAAGCCGCTACCGCAGGGGCGGCGTTGTGCTGCCATAAGGGCGCACAGTTGCACTGCGC‑GTGGTGTGGATAGTCGGGCAACAAATGTTTCGTTAATc                                                                  >  8:183226/4‑149 (MQ=255)
                                                                        cACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTaa                                                                    >  2:102819/1‑147 (MQ=255)
                                                                         aCGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCg                                                                 <  4:300745/149‑1 (MQ=255)
                                                                         aCGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCg                                                                 >  2:103715/1‑149 (MQ=255)
                                                                            ccGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGcac                                                              <  1:103715/149‑1 (MQ=255)
                                                                               aCGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGa                                                           <  6:313882/149‑1 (MQ=255)
                                                                                   aaCCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGt                                                       >  5:191853/1‑149 (MQ=255)
                                                                                    aCCTGAGGTCGAGGGCCGCGTTGCGGACCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCTTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GGGGGGGGGATAGGCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTc                                                      <  7:183226/149‑1 (MQ=255)
                                                                                    aCCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTc                                                      <  6:333849/149‑1 (MQ=255)
                                                                                      cTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGa                                                    <  6:191853/149‑1 (MQ=255)
                                                                                       tGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGAt                                                   >  7:121697/1‑149 (MQ=255)
                                                                                            gaatgggcCGCAACGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCg                                              >  2:162890/5‑149 (MQ=255)
                                                                                                 ggcCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTg                                         >  5:36701/1‑149 (MQ=255)
                                                                                                    cgcgTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGt                                        >  7:229403/1‑147 (MQ=255)
                                                                                                     gcgTTGCGGCCCATCCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAg                                     <  3:367320/149‑1 (MQ=255)
                                                                                                       gTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGt                                   <  6:36701/149‑1 (MQ=255)
                                                                                                            ggCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGtaccta                              <  3:90705/149‑1 (MQ=255)
                                                                                                             gCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGAtt                                          <  1:37712/136‑1 (MQ=255)
                                                                                                             gCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGAtt                                          >  2:37712/1‑136 (MQ=255)
                                                                                                             gCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGtacctac                             >  1:370137/1‑149 (MQ=255)
                                                                                                              cccATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGAt                                                   <  4:341640/126‑1 (MQ=255)
                                                                                                              cccATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGAt                                                   >  3:341640/1‑126 (MQ=255)
                                                                                                               ccATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCa                           <  7:290677/149‑1 (MQ=255)
                                                                                                                      caGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCt                    <  3:135814/149‑1 (MQ=255)
                                                                                                                       aGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAgg                                    <  5:227313/132‑1 (MQ=255)
                                                                                                                       aGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAgg                                    >  6:227313/1‑132 (MQ=255)
                                                                                                                         cacaaCGCCGCCCCTGCGGGAGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTaa                 >  7:33888/4‑149 (MQ=255)
                                                                                                                            aaCGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCt              >  3:73284/1‑149 (MQ=255)
                                                                                                                               gccgcTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTAAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTggg           <  8:229403/149‑1 (MQ=255)
                                                                                                                               gacgcTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGGGGATAGGCGTGCAACAAATGGTTCGTTACTAGCACAGATAGTCTAGTATCGGATTGTAAGGTCACTACCAGTCTCCTTAATATggg           >  1:112234/3‑149 (MQ=255)
                                                                                                                                  gcTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTCAGGTACCTACCAGTCTCCTTAATCTGGGtg         >  7:129505/1‑147 (MQ=255)
                                                                                                                                       cGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGCAGTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTAcc     >  1:87252/1‑148 (MQ=255)
                                                                                                                                        gCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTAc      >  6:303867/1‑145 (MQ=255)
                                                                                                                                        gCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTAc      <  5:303867/145‑1 (MQ=255)
                                                                                                                                        gCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTACCGaa  <  1:162890/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
CAGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTACCGAA  >  NC_002947/1568774‑1569058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑