Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,568,915 G→A 7.6% intergenic (+52/‑275) pcaK → / → pcaF‑I 4‑hydroxybenzoate transporter/beta‑ketoadipyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,568,9150GA 7.6% 191.7 / 4.8 66intergenic (+52/‑275)pcaK/pcaF‑I4‑hydroxybenzoate transporter/beta‑ketoadipyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base A (2/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

CAGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTACCGAAGCACC  >  NC_002947/1568774‑1569063
                                                                                                                                             |                                                                                                                                                     
cAGGTGCTGAGGGCGTTGCTGGTACCGGCGGCGCTGGGGACCGTGGGAGTGGTGGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCACATTCACAGCGTAACGCCGCCCCTGCGGGAgcggcg                                                                                                                                                 <  6:371113/148‑1 (MQ=255)
 aGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGtt                                                                                                                                               >  1:283682/1‑149 (MQ=255)
 aGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGtt                                                                                                                                               <  6:245955/149‑1 (MQ=255)
   gTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTtgt                                                                                                                                             >  8:290677/1‑149 (MQ=255)
    tGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTtgtg                                                                                                                                            <  7:257086/149‑1 (MQ=255)
      cTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCCCCTGCGGGAGCGGCGttacgct                                                                                                                                          <  5:287745/149‑6 (MQ=255)
       tGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGtgctg                                                                                                                                         >  6:102067/1‑149 (MQ=255)
          cGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAAGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCa                                                                                                                                       >  2:169286/1‑148 (MQ=255)
           ggCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAAt                                                                                                                                     >  8:177170/1‑149 (MQ=255)
            gCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAAt                                                                                                                                     >  4:90705/1‑148 (MQ=255)
            gCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATc                                                                                                                                    <  6:342078/149‑1 (MQ=255)
                 gCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGgc                                                                                                                               >  3:300745/1‑149 (MQ=255)
                  cTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGgc                                                                                                                               <  2:20246/148‑1 (MQ=255)
                  cTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGgc                                                                                                                               <  2:283682/148‑1 (MQ=255)
                         cggcggCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGtt                                                                                                                       <  6:68071/149‑1 (MQ=255)
                           gcggcgCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCACACAGTTGc                                                                                                                     <  3:145110/149‑1 (MQ=255)
                                    gCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑Gt                                                                                                           >  4:135814/1‑149 (MQ=255)
                                      gACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCACAACGCCGCCCCTGCGGGAGCGGCGttacgc                                                                                                                                           >  8:110924/1‑112 (MQ=37)
                                      gACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCACAACGCCGCCCCTGCGGGAGCGGCGttacgc                                                                                                                                           <  7:110924/116‑5 (MQ=37)
                                      gACCGGGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTgg                                                                                                         <  6:368426/149‑1 (MQ=255)
                                         cGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGt                                                                                                      <  7:147519/149‑1 (MQ=255)
                                         cGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGt                                                                                                      <  3:61994/149‑1 (MQ=255)
                                          gTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTg                                                                                                     >  8:27755/1‑149 (MQ=255)
                                            gggAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGa                                                                                                   >  8:14758/1‑149 (MQ=255)
                                                   gtcgtcAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGt                                                                                            >  5:313882/1‑149 (MQ=255)
                                                   gtcgtcAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGt                                                                                            <  2:113038/149‑1 (MQ=255)
                                                   gtcgtcAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATCCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGt                                                                                            >  4:367320/1‑149 (MQ=255)
                                                        cAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGcaa                                                                                        >  8:148239/1‑148 (MQ=255)
                                                                  gTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGtt                                                                               <  3:345445/147‑1 (MQ=255)
                                                                  gTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGtt                                                                               <  5:102067/147‑1 (MQ=255)
                                                                  gTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTc                                                                              <  1:169286/148‑1 (MQ=255)
                                                                        ccagCCGACGCAAACTGAGGGCGAGGTCCGCGTTGCGGCCCAGTCAAAGCGTAAAGCCGCTACCGCAGGGGCGGCGTTGTGCTGCCATAAGGGCGCACAGTTGCACTGCGC‑GTGGTGTGGATAGTCGGGCAACAAATGTTTCGTTAATc                                                                       >  8:183226/4‑149 (MQ=255)
                                                                        cACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTaa                                                                         >  2:102819/1‑147 (MQ=255)
                                                                         aCGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCg                                                                      <  4:300745/149‑1 (MQ=255)
                                                                         aCGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCg                                                                      >  2:103715/1‑149 (MQ=255)
                                                                            ccGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGcac                                                                   <  1:103715/149‑1 (MQ=255)
                                                                               aCGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGa                                                                <  6:313882/149‑1 (MQ=255)
                                                                                   aaCCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGt                                                            >  5:191853/1‑149 (MQ=255)
                                                                                    aCCTGAGGTCGAGGGCCGCGTTGCGGACCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCTTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GGGGGGGGGATAGGCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTc                                                           <  7:183226/149‑1 (MQ=255)
                                                                                    aCCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTc                                                           <  6:333849/149‑1 (MQ=255)
                                                                                      cTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGa                                                         <  6:191853/149‑1 (MQ=255)
                                                                                       tGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGAt                                                        >  7:121697/1‑149 (MQ=255)
                                                                                            gaatgggcCGCAACGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCg                                                   >  2:162890/5‑149 (MQ=255)
                                                                                                 ggcCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTg                                              >  5:36701/1‑149 (MQ=255)
                                                                                                    cgcgTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGt                                             >  7:229403/1‑147 (MQ=255)
                                                                                                     gcgTTGCGGCCCATCCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAg                                          <  3:367320/149‑1 (MQ=255)
                                                                                                       gTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGt                                        <  6:36701/149‑1 (MQ=255)
                                                                                                            ggCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGtaccta                                   <  3:90705/149‑1 (MQ=255)
                                                                                                             gCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGAtt                                               <  1:37712/136‑1 (MQ=255)
                                                                                                             gCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGAtt                                               >  2:37712/1‑136 (MQ=255)
                                                                                                             gCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGtacctac                                  >  1:370137/1‑149 (MQ=255)
                                                                                                              cccATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGAt                                                        <  4:341640/126‑1 (MQ=255)
                                                                                                              cccATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGAt                                                        >  3:341640/1‑126 (MQ=255)
                                                                                                               ccATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCa                                <  7:290677/149‑1 (MQ=255)
                                                                                                                      caGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCt                         <  3:135814/149‑1 (MQ=255)
                                                                                                                       aGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAgg                                         <  5:227313/132‑1 (MQ=255)
                                                                                                                       aGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAgg                                         >  6:227313/1‑132 (MQ=255)
                                                                                                                         cacaaCGCCGCCCCTGCGGGAGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTaa                      >  7:33888/4‑149 (MQ=255)
                                                                                                                            aaCGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCt                   >  3:73284/1‑149 (MQ=255)
                                                                                                                               gccgcTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTAAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTggg                <  8:229403/149‑1 (MQ=255)
                                                                                                                               gacgcTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGGGGATAGGCGTGCAACAAATGGTTCGTTACTAGCACAGATAGTCTAGTATCGGATTGTAAGGTCACTACCAGTCTCCTTAATATggg                >  1:112234/3‑149 (MQ=255)
                                                                                                                                  gcTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTCAGGTACCTACCAGTCTCCTTAATCTGGGtg              >  7:129505/1‑147 (MQ=255)
                                                                                                                                       cGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGCAGTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTAcc          >  1:87252/1‑148 (MQ=255)
                                                                                                                                        gCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTAc           >  6:303867/1‑145 (MQ=255)
                                                                                                                                        gCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTAc           <  5:303867/145‑1 (MQ=255)
                                                                                                                                        gCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTACCGaa       <  1:162890/149‑1 (MQ=255)
                                                                                                                                             ggcggcGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTACCGAAGCAcc  <  8:121697/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
CAGGTGCTGACGGCGTTGCTGGTACCGGCGGCGCTGGCGACCGTGGGAGTGGTCGTCAAGGGGCTGGTCAGCCACGCCGACGCAACCTGAGGGCGAGGGCCGCGTTGCGGCCCATTCACAGCGTAACGCCGCTCCCGCAGGGGCGGCGTTGTGCTGCAATCAGGGCGCACAGTTGCCCTGCGC‑GTGGGGTGGATAGTCGTGCAACAAATGGTTCGTTAATCGCACAGATAGTCGATTATCGGATTGTAAGGTACCTACCAGTCTCCTTAATCTGGGTTTACCGAAGCACC  >  NC_002947/1568774‑1569063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑