Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,575,211	G→T	10.5%	intergenic (‑197/+13)	pcaP ← / ← galP‑II	porin/porin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,575,211	0	G	T	10.5%	224.4 / 13.6	76	intergenic (‑197/+13)	pcaP/galP‑II	porin/porin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (34/34);  new base T (5/3);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

GGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGCACCACATAGGCC  >  NC_002947/1575065‑1575359                                                                                                                                                    |                                                                                                                                                     ggATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAagag                                                                                                                                                    <  3:223022/149‑1 (MQ=255)       ccTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAAt                                                                                                                                              >  4:310927/1‑149 (MQ=255)        cTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                                             >  8:321198/1‑149 (MQ=255)           cAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                                           <  5:285412/148‑1 (MQ=255)                accaccATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCAGCGAAGAGGCCAATTGAGTcaca                                                                                                                                     >  3:73335/1‑149 (MQ=255)                 ccaccaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTC‑TTCGCGGATAAACCCGCGAATAGGCCaga                                                                                                                                              >  6:340974/1‑137 (MQ=38)                 ccaccaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTC‑TTCGCGGATAAACCCGCGAATAGGCCaga                                                                                                                                              <  5:340974/139‑3 (MQ=38)                 ccaccaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTca                                                                                                                                       >  5:365680/1‑146 (MQ=255)                 ccaccaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACag                                                                                                                                    <  3:334881/149‑1 (MQ=255)                   accaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaa                                                                                                                                  <  6:35197/149‑1 (MQ=255)                            agctcagcGAATGGCGGCAGCTCTCACTGGGGAATTCATGGCAGGGTTTTCATCACTCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCTTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGATGCCAATTGAGTCACAGAAGATTCAGc                                                                                                                          <  2:129519/148‑1 (MQ=255)                            agctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGCGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGTGGCCAATTGAGTCACAGAAGATTCAg                                                                                                                           <  6:219273/147‑1 (MQ=255)                            agctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGCGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGTGGCCAATTGAGTCACAGAAGATTCAg                                                                                                                           >  5:219273/1‑147 (MQ=255)                            agctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCg                                                                                                                         <  1:35897/149‑1 (MQ=255)                             gctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAg                                                                                                                                          <  8:173230/131‑1 (MQ=255)                             gctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAg                                                                                                                                          >  7:173230/1‑131 (MQ=255)                                 agcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATaa                                                                                                                    <  7:321198/149‑1 (MQ=255)                                 agcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATaa                                                                                                                    >  7:162430/1‑149 (MQ=255)                                    gAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACt                                                                                                                  >  4:98281/1‑148 (MQ=255)                                     aaTGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTca                                                                                                                >  5:96973/1‑149 (MQ=255)                                      aTGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTcac                                                                                                               <  2:150890/149‑1 (MQ=255)                                            gcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATc                                                                                                           >  7:16804/1‑147 (MQ=255)                                                cTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCgg                                                                                                     <  8:162430/149‑1 (MQ=255)                                                   gCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtc                                                                                                  >  5:39232/1‑149 (MQ=255)                                                     aCTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGc                                                                                                                          <  1:203559/123‑1 (MQ=255)                                                     aCTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGc                                                                                                                          >  2:203559/1‑123 (MQ=255)                                                     aCTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtct                                                                                                 >  5:96365/1‑148 (MQ=255)                                                       tGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCAt                                                                                              >  4:72757/1‑149 (MQ=255)                                                         tgCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATc                                                                                             <  4:110815/147‑1 (MQ=255)                                                         ggCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCAt                                                                                              <  2:153002/147‑1 (MQ=255)                                                             aTTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAAcg                                                                                        >  2:95439/1‑149 (MQ=255)                                                                    gCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGt                                                                                 <  3:310927/149‑1 (MQ=255)                                                                      aGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                                           >  7:249956/1‑105 (MQ=255)                                                                      aGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                                           <  8:249956/105‑1 (MQ=255)                                                                       gggTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCt                                                                              <  6:96973/149‑1 (MQ=255)                                                                          ttttCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGaa                                                                           <  6:365680/149‑1 (MQ=255)                                                                            ttCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGt                                                                         >  7:44545/1‑149 (MQ=255)                                                                              catcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTc                                                                        <  2:30840/148‑1 (MQ=255)                                                                                tcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGaa                                                                           <  8:193376/143‑1 (MQ=255)                                                                                tcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGaa                                                                           >  7:193376/1‑143 (MQ=255)                                                                                tcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTcgc                                                                      >  2:180206/1‑148 (MQ=255)                                                                                    gCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACg                                                                  >  4:124934/1‑148 (MQ=255)                                                                                          gCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTg                                                                                   >  5:104165/1‑125 (MQ=255)                                                                                          gCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTg                                                                                   <  6:104165/125‑1 (MQ=255)                                                                                          gCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGt                                                            <  3:98281/148‑1 (MQ=255)                                                                                           ccAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTc                                                           <  6:96365/148‑1 (MQ=255)                                                                                            cAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTc                                                           >  1:241788/1‑148 (MQ=255)                                                                                            cAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTc                                                           >  5:191795/1‑147 (MQ=255)                                                                                                 atgCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgc                                                    <  8:16804/149‑1 (MQ=255)                                                                                                    ccTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGtt                                                      <  2:3542/144‑1 (MQ=255)                                                                                                    ccTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGtt                                                      >  1:3542/1‑144 (MQ=255)                                                                                                     cTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaa                                                                                                                                  >  6:88782/1‑67 (MQ=255)                                                                                                     cTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                                           >  5:238884/1‑74 (MQ=255)                                                                                                     cTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                                           <  6:238884/74‑1 (MQ=255)                                                                                                     cTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTcgc                                                                      >  7:327470/1‑127 (MQ=255)                                                                                                     cTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTcgc                                                                      <  8:327470/127‑1 (MQ=255)                                                                                                         aaaaGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAttt                                             >  6:287152/1‑148 (MQ=255)                                                                                                                  ggCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAg                                    <  1:180206/148‑1 (MQ=255)                                                                                                                     gTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTcc                                <  1:137407/149‑1 (MQ=255)                                                                                                                          gCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCtt                              <  8:44545/146‑1 (MQ=255)                                                                                                                           ccTC‑TTCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGc                                         <  8:264685/134‑1 (MQ=255)                                                                                                                           ccTC‑TTCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGc                                         >  7:264685/1‑134 (MQ=255)                                                                                                                           ccTC‑TTCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAg                          >  2:181859/1‑149 (MQ=255)                                                                                                                             ctcttCGCGGATAAACCCGCGATTAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGc                         >  3:368256/4‑149 (MQ=255)                                                                                                                                ttCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTg                       <  6:39232/148‑1 (MQ=255)                                                                                                                                ttCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGc                      >  6:272650/1‑149 (MQ=255)                                                                                                                                  cgcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGccc                    >  6:293221/1‑149 (MQ=255)                                                                                                                                  cgcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGcc                     <  5:287152/148‑1 (MQ=255)                                                                                                                                  cgcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGcc                     <  7:29810/148‑1 (MQ=255)                                                                                                                                   gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGccc                    <  1:329126/148‑1 (MQ=255)                                                                                                                                   gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGccc                    >  6:271141/1‑148 (MQ=255)                                                                                                                                   gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGAGCTGAAGTCGCGACGCATGGTCGa                                                         >  8:145857/1‑111 (MQ=255)                                                                                                                                     gggTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGAc                 <  3:72757/149‑1 (MQ=255)                                                                                                                                       gTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTg               >  1:186459/1‑149 (MQ=255)                                                                                                                                       ataaacccGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCAGACTg               >  1:337417/7‑149 (MQ=255)                                                                                                                                            tCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGcacca          >  5:130181/1‑149 (MQ=255)                                                                                                                                                 gAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGCACCACATAg     <  1:181859/149‑1 (MQ=255)                                                                                                                                                   agagGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGCACCACATAGGc   <  2:241788/149‑1 (MQ=255)                                                                                                                                                    gagGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGCACCACATAGGcc  <  6:191795/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTA‑TTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGCACCACATAGGCC  >  NC_002947/1575065‑1575359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑