Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,578,379 G→A 7.8% G1006G (GGC→GGT)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,578,3790GA 7.8% 146.5 / 2.1 51G1006G (GGC→GGT) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base G (25/22);  new base A (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAAAGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATGCGGCACGCCTCGATCGCTGCGTCGTAC  >  NC_002947/1578232‑1578526
                                                                                                                                                    |                                                                                                                                                   
actatctcTTGGTATTTGCTCAGTCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATgccgc                                                                                                                                                    <  6:260185/142‑1 (MQ=255)
 caaAGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTTTTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATgccgc                                                                                                                                                    >  1:295392/1‑149 (MQ=255)
 caaAGACTTGGTCATTGCCCAGACTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACACAGAAGATAGCCAGCACGGTCGCACTGATCATgccgcc                                                                                                                                                   >  7:56704/1‑149 (MQ=255)
  aaaGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATgccgcc                                                                                                                                                   >  2:42807/1‑148 (MQ=255)
     gACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGAt                                                                                                                                                >  8:131833/1‑148 (MQ=255)
      aCTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCa                                                                                                                                              <  7:106145/149‑1 (MQ=255)
       cTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCAc                                                                                                                                             <  7:131833/149‑1 (MQ=255)
        ttGGTCATTGCCCAGCCTCCTAACGTGGATTTTCAGGGGTGACGTCTTTTTACGTC‑TCTTTGCTGCCGCACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATgccgccagctcg                                                                                                                                             >  5:260185/1‑142 (MQ=255)
                   ccAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGAt                                                                                                                                 <  1:297058/149‑1 (MQ=255)
                   ccAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCACCGATCACGCCAGTACCGAt                                                                                                                                 >  6:140238/1‑149 (MQ=255)
                           aTAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTgc                                                                                                                          <  1:42807/148‑1 (MQ=255)
                              aCGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCACCGATCACGCCAGTACCGATGGCGTgctggc                                                                                                                      >  6:245415/1‑149 (MQ=255)
                                gTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTgctggctg                                                                                                                    >  3:257537/1‑149 (MQ=255)
                                gTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTgctggctg                                                                                                                    >  7:92462/1‑149 (MQ=255)
                                                  cGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGc                                                                                                                              <  4:177305/121‑1 (MQ=255)
                                                  cGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGc                                                                                                                              >  3:177305/1‑121 (MQ=255)
                                                   gTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCAGGCGCCGGCGCCGCTgg                                                                                                 >  6:197991/1‑149 (MQ=255)
                                                    tCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGc                                                                                                >  3:174261/1‑149 (MQ=255)
                                                      tttttCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGa                                                                                              >  2:84394/1‑149 (MQ=255)
                                                         ttCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATg                                                                                            <  8:270264/148‑1 (MQ=255)
                                                              gC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTAc                                                                                                                                     >  3:60849/1‑102 (MQ=255)
                                                              gC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTAc                                                                                                                                     <  4:60849/102‑1 (MQ=255)
                                                              gC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAAc                                                                                      >  4:345753/1‑149 (MQ=255)
                                                                 tctTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACgg                                                                                    >  8:33317/1‑149 (MQ=255)
                                                                  ctTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGt                                                                                   <  8:56704/149‑1 (MQ=255)
                                                                   tttGCTGCCGAACAGCGACGACCCTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCggcgccggcgcc                                                                                                      <  1:34962/129‑1 (MQ=255)
                                                                   tttGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCggcgccggcgcc                                                                                                      >  2:34962/1‑129 (MQ=255)
                                                                     tgctgcCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCACCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGTGATGGTCAACGGTa                                                                                  <  5:245415/147‑1 (MQ=255)
                                                                       ctgcCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCAc                                                                              <  1:84394/149‑1 (MQ=255)
                                                                        tgcCGAACAGTGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTgctgg                                                                                                                       >  2:233820/1‑107 (MQ=255)
                                                                          ccGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGcc                                                                           >  6:172088/1‑149 (MQ=255)
                                                                           cGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCaa                                                                                       >  4:134313/1‑136 (MQ=255)
                                                                           cGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCaa                                                                                       <  3:134313/136‑1 (MQ=255)
                                                                            gAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCa                                                                         <  1:92884/149‑1 (MQ=255)
                                                                                 gCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATgg                                                                                           <  8:341487/126‑1 (MQ=255)
                                                                                 gCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATgg                                                                                           >  7:341487/1‑126 (MQ=255)
                                                                                 gCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGAt                                                                     >  8:310127/1‑148 (MQ=255)
                                                                                    acgacACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAAc                                                                 >  4:21840/1‑149 (MQ=255)
                                                                                     cgacACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACg                                                                >  6:226515/1‑149 (MQ=255)
                                                                                        cacTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCa                                                             <  7:310127/149‑1 (MQ=255)
                                                                                        cacTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCAGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCa                                                             <  5:197991/149‑1 (MQ=255)
                                                                                          cTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCACCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGc                                                           <  5:140238/149‑1 (MQ=255)
                                                                                             cgacgaAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAg                                                        <  2:295392/149‑1 (MQ=255)
                                                                                                cgaAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGt                                                      >  2:90391/1‑148 (MQ=255)
                                                                                                    gaaCAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCatga                                                 >  2:161771/1‑149 (MQ=255)
                                                                                                    gaaCAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCatga                                                 <  4:257537/149‑1 (MQ=255)
                                                                                                                ccAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGATCGCCAGCGAGGTCATGATGATCGGGCGCa                                     <  1:214361/149‑1 (MQ=255)
                                                                                                                   gaagaTAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGAc                                  <  3:345753/149‑1 (MQ=255)
                                                                                                                        tAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATg                             <  1:161771/149‑1 (MQ=255)
                                                                                                                            cAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATGCgg                          >  3:70207/1‑148 (MQ=255)
                                                                                                                             aGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATGCGGCa                        >  8:38852/1‑149 (MQ=255)
                                                                                                                              gCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATGCGGCAc                       >  5:216338/1‑149 (MQ=255)
                                                                                                                                  ggTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATGCGGCACGCCt                   <  4:174261/149‑1 (MQ=255)
                                                                                                                                       cACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATGCGGCACGCCTCGATc              <  6:216338/149‑1 (MQ=255)
                                                                                                                                        aCTGATCATGCCGCCGATCACGCCAGAACCGATGGCGTGCTGGCAGCCGGCGCCGGCGCCGCTGGCGATGGAAAACGGAACCACG‑ACAGGATGAACGCCAGCGAGGACAAGATGATCGCGCCCAGACGCATGCGGCACGCCGAGATCGc            >  7:232424/1‑149 (MQ=255)
                                                                                                                                            aTCATGCCGCCGATCACGCCAGTACAGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGACACCGAGGTCATGATGATCGGGCGCAg                                    >  5:38619/1‑122 (MQ=255)
                                                                                                                                                  cggccgATCACGCCTGTACCGATGGCGTGCTGGCTGCCGGGTCCGGCGCCGCTGGCGATGGTCAACTGTACCACGCCCATGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATGCGGCACGCCTCGATCGCTGCGTCGTa   <  5:172088/147‑1 (MQ=255)
                                                                                                                                                  ccgccgATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATGCGGCACGCCTCGATCGCTGCGTCGTa   >  8:343254/1‑149 (MQ=255)
                                                                                                                                                   cgccgATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATGCGGCACGCCTCGATCGCTGCGTCGTAc  <  1:90391/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACAAAGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGC‑TCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCAGACGCATGCGGCACGCCTCGATCGCTGCGTCGTAC  >  NC_002947/1578232‑1578526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑