Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,580,995 C→A 100% K134N (AAG→AAT)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,580,9950CA94.1% 144.6 / 0.9 51K134N (AAG→AAT) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (22/26);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCAT  >  NC_002947/1580849‑1581135
                                                                                                                                                  |                                                                                                                                             
gCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGttatt                                                                                                                                             <  4:283177/149‑1 (MQ=255)
         gcACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCttt                                                                                                                                    <  7:13682/149‑1 (MQ=255)
                 cacCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTGCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGAcgc                                                                                                                            <  5:318303/149‑1 (MQ=255)
                       gAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGAt                                                                                                                        <  7:115740/147‑1 (MQ=255)
                              ccTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgctg                                                                                                               >  8:292362/1‑149 (MQ=255)
                               cTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgctgc                                                                                                              <  6:164758/149‑1 (MQ=255)
                                  cacCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgctgctgc                                                                                                           <  2:45224/149‑1 (MQ=255)
                                   acCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCTCTGCTTTGGTGACGCGGa                                                                                                                         <  3:324049/134‑1 (MQ=255)
                                   acCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGa                                                                                                                         >  4:324049/1‑134 (MQ=255)
                                   acCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCa                                                                                                          >  5:322830/1‑149 (MQ=255)
                                     ccGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgc                                                                                                                 <  1:158335/140‑1 (MQ=255)
                                     ccGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgc                                                                                                                 >  2:158335/1‑140 (MQ=255)
                                     ccGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACt                                                                                                        >  2:337428/1‑149 (MQ=255)
                                     ccGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACt                                                                                                        >  1:314724/1‑149 (MQ=255)
                                      cgcgGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACtt                                                                                                       >  5:145010/1‑149 (MQ=255)
                                         ggTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCt                                                                                                    <  5:160567/149‑1 (MQ=255)
                                                  gaTCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCGTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGgcagca                                                                                           >  6:125598/1‑149 (MQ=255)
                                                    tCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCagcag‑c                                                                                        >  2:33405/1‑149 (MQ=255)
                                                           cTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGTCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTgg                                                                                 <  6:196908/149‑1 (MQ=255)
                                                            tGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGc                                                                                >  3:202065/1‑149 (MQ=255)
                                                              cATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCGTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGc                                                                                >  5:27818/1‑147 (MQ=255)
                                                                tGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCAc                                                                                                         >  2:363950/1‑121 (MQ=255)
                                                                tGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCAc                                                                                                         <  1:363950/121‑1 (MQ=255)
                                                                   tCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGtt                                                                          >  7:106972/1‑148 (MQ=255)
                                                                     gagaCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAg                                                                       >  8:301225/1‑149 (MQ=255)
                                                                       gaCGATATAGTTTGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCTGAGACCAGGCCGATAACAAGCAGGAAGTTATTCACTGATTTGCTGACGCGGATAACTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCt                                                                     >  2:65014/1‑149 (MQ=255)
                                                                       gaCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCt                                                                     <  1:337428/149‑1 (MQ=255)
                                                                         cGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTg                                                                   <  6:145010/149‑1 (MQ=255)
                                                                            tataGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTc                                                                <  4:202065/149‑1 (MQ=255)
                                                                              taGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTct                                                               <  8:106972/148‑1 (MQ=255)
                                                                                gTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTctgt                                                             <  7:292362/148‑1 (MQ=255)
                                                                                 ttGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGgcagca                                                                                           <  2:308584/118‑1 (MQ=255)
                                                                                 ttGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCGGCGgcagca                                                                                           >  1:308584/1‑118 (MQ=255)
                                                                                   ggCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCCGGGGTGGCCAGGTTCAGCTTGTTctgtac                                                           <  1:33405/148‑1 (MQ=255)
                                                                                     ccAGGTCGTCCTTGGTCATGCTGCCGTCTTAGGATAAAAGGCCGATCACAAGCAGGAAGTTATTGAATGCTTTGGTGACGTGGATAAATTGCTGCTGCACTTGCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCATGTTGTTctttagctg                                                        >  6:235375/1‑148 (MQ=255)
                                                                                        ggTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTCtgtacctgtacc                                                    <  7:301225/149‑1 (MQ=255)
                                                                                                  ggTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGgt                                            <  6:322830/147‑1 (MQ=255)
                                                                                                               tCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGt                                                                                   <  5:207457/95‑1 (MQ=255)
                                                                                                               tCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGt                                                                                   >  6:207457/1‑95 (MQ=255)
                                                                                                                 ttCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCt                                                                     <  2:47058/107‑1 (MQ=255)
                                                                                                                 ttCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCt                                                                     >  1:47058/1‑107 (MQ=255)
                                                                                                                           aGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTAGGTGCCATGTTCGaa                    >  4:48824/1‑146 (MQ=255)
                                                                                                                           aGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGGTCTGTACCTGTACCTGCGCGGTGTCGGGGTGGGTGCCCTGTTCGaa                    <  3:48824/146‑1 (MQ=255)
                                                                                                                            ggCCGATCACCAGCAGGAAGTTATTCAGTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGttacgtaag                 >  7:197477/1‑141 (MQ=255)
                                                                                                                            ggCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTg                >  6:201752/1‑149 (MQ=255)
                                                                                                                              ccGATGACCAGCAGGAAGTTATTCACTTCTTTGGTGACGCTGATACCTTTCTGCTGCACTTGCTGCGTCAGCAG‑CGGGGTTGCCAGGTTCAGCTTGTGCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGc              <  1:65014/149‑1 (MQ=255)
                                                                                                                                     ccAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGTGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTgcgc                                               >  3:161023/1‑109 (MQ=255)
                                                                                                                                     ccAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGTGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTgcgc                                               <  4:161023/109‑1 (MQ=255)
                                                                                                                                     ccAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATg       <  2:314724/149‑1 (MQ=255)
                                                                                                                                     ccAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAACAG‑CGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATg       >  1:111348/1‑149 (MQ=255)
                                                                                                                                      cagcagGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATgg      <  5:201752/149‑1 (MQ=255)
                                                                                                                                          agGAAGTTCGTCACTGCTTTGGTGACGCGGATAGCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGATTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCAt  <  5:125598/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
GCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAG‑CGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCAT  >  NC_002947/1580849‑1581135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑