Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,606,407 A→T 10.4% intergenic (‑157/+54) PP_1407 ← / ← PP_t64 sulfate transporter/tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,606,4070AT10.4% 145.2 / 3.5 54intergenic (‑157/+54)PP_1407/PP_t64sulfate transporter/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base A (23/25);  new base T (4/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTT  >  NC_002947/1606269‑1606552
                                                                                                                                          |                                                                                                                                                 
gTTTCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAAGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCtt                                                                                                                                          <  5:64101/148‑1 (MQ=255)
 tttCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCtttt                                                                                                                                        >  3:184418/1‑149 (MQ=255)
 tttCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTCGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCTTGGCTCCtttt                                                                                                                                        >  8:261558/1‑149 (MQ=255)
   tCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAATAAGGAGCCACGGCTCCTTTTGa                                                                                                                                      >  4:305702/1‑149 (MQ=255)
     aCGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACt                                                                                                                                    >  2:339798/1‑149 (MQ=255)
       gAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGc                                                                                                                                  >  4:195035/1‑149 (MQ=255)
        aaTCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCTTGGCTCCttttttcgttt                                                                                                                                 <  7:261558/149‑8 (MQ=255)
          tCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGAt                                                                                                                               <  3:24989/149‑1 (MQ=255)
            cAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGTCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGa                                                                                                                             >  2:317617/1‑149 (MQ=255)
              gTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACg                                                                                                                           >  5:249835/1‑149 (MQ=255)
                       tCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACAGCGATTAAGttcatgcac                                                                                                                  >  6:258818/1‑142 (MQ=255)
                       tCCGCCACAAGCCGGTTCGCTTTCGCACGCAAAGGCCCTATGCGCATTTAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGaa                                                                                                                  <  6:370538/149‑1 (MQ=255)
                           ccACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGc                                                                                                               >  4:77379/1‑148 (MQ=255)
                                   cGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGTCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGa                                                                                                      <  1:317617/149‑1 (MQ=255)
                                   cGTTTCGCTGTCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGTCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGa                                                                                                      <  8:277942/149‑1 (MQ=255)
                                    gTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGAc                                                                                                     >  4:211754/1‑149 (MQ=255)
                                    gTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGAc                                                                                                     >  4:355264/1‑149 (MQ=255)
                                          cTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGAt                                                                                               <  3:211754/149‑1 (MQ=255)
                                              cgcacgcaAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGAtgtg                                                                                            <  1:339798/148‑1 (MQ=255)
                                                 acgcaAAGGCCCGATGCTCATTGAAGCGGATCGGTGGGTAATTGCCCATGCGCCAGGTCAAGGCCGGATGTAAAACGAAATAAGGAGGCAAGGCTCCTTTTGACTGGGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGTAGc                                                                                        <  5:258818/149‑1 (MQ=255)
                                                 acgcaAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGc                                                                                        >  7:328164/1‑149 (MQ=255)
                                                   gcaAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAGGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCgg                                                                                      >  6:218650/1‑149 (MQ=255)
                                                   gcaAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCgg                                                                                      <  5:150956/149‑1 (MQ=255)
                                                          cccGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAGGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAgg                                                                               <  5:218650/149‑1 (MQ=255)
                                                          cccGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAgg                                                                               <  4:107021/149‑1 (MQ=255)
                                                                    aTTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGc                                                                                        <  8:187355/130‑1 (MQ=255)
                                                                    aTTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGc                                                                                        >  7:187355/1‑130 (MQ=255)
                                                                    aTTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAAcc                                                                     <  2:52736/149‑1 (MQ=255)
                                                                        aaGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTc                                                                  <  3:195035/148‑1 (MQ=255)
                                                                        aaGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTc                                                                  <  6:249835/148‑1 (MQ=255)
                                                                         aGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGaa                                                                                                                  >  4:292718/1‑99 (MQ=255)
                                                                         aGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGaa                                                                                                                  <  3:292718/99‑1 (MQ=255)
                                                                              aTCGGTGGGTAATTGGCCATGGGCCAGGTCAAGTCAGGATGAAAAACGAAAAAAGGAGCCTAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTaaa                                                           <  1:262993/149‑1 (MQ=255)
                                                                                  gTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCtt                                                       >  1:74875/1‑149 (MQ=255)
                                                                                  gTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCtt                                                       <  8:232333/149‑1 (MQ=255)
                                                                                   tGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTg                                                      <  8:132720/149‑1 (MQ=255)
                                                                                                   ggCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGtttt                                       <  7:136862/148‑1 (MQ=255)
                                                                                                   ggCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTCCCAAGCTTTAGttgag                                      <  4:184418/149‑4 (MQ=255)
                                                                                                      cAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCa                                   <  3:355264/149‑1 (MQ=255)
                                                                                                        ggTCAAGGCAGGATGAAAAACGAAAAAAGGAGACAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCAc                                  >  6:319297/1‑148 (MQ=255)
                                                                                                         gTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTCCCAAGCTTTAGt                                          <  3:161016/139‑1 (MQ=255)
                                                                                                         gTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTCCCAAGCTTTAGt                                          >  4:161016/1‑139 (MQ=255)
                                                                                                            aaGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCGTGGGAAGCTTTCGTTTTGCCAcgtaac                             >  4:234043/1‑144 (MQ=255)
                                                                                                                 aGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGACCCCTCAACTAAAGCTTGGGACGCTTTCGTTGTGCGACTAAACTAGAc                        >  4:68552/1‑149 (MQ=255)
                                                                                                                    aTGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCg                     >  5:302602/1‑149 (MQ=255)
                                                                                                                         aaaCGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCt                >  7:282372/1‑149 (MQ=255)
                                                                                                                           aCGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGa              <  6:302602/149‑1 (MQ=255)
                                                                                                                             gAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGATTCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGc            >  8:332876/1‑149 (MQ=255)
                                                                                                                             gAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGc            <  8:328164/149‑1 (MQ=255)
                                                                                                                                aaaaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTc          >  3:263033/1‑148 (MQ=255)
                                                                                                                                aaaaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTc          >  8:11614/1‑148 (MQ=255)
                                                                                                                                  aaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGAc       >  7:274751/1‑149 (MQ=255)
                                                                                                                                  aaGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGAc       >  7:90076/1‑149 (MQ=255)
                                                                                                                                       gCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACttttt  <  5:319297/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
GTTTCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTT  >  NC_002947/1606269‑1606552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑