Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,606,502	G→C	7.4%	noncoding (33/74 nt)	PP_t64 ←	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,606,502	0	G	C	7.4%	158.4 / 4.4	54	noncoding (33/74 nt)	PP_t64	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (27/23);  new base C (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

GGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTATTGGCTACAAGGCGCCGAGCCGCT  >  NC_002947/1606368‑1606646                                                                                                                                       |                                                                                                                                                 ggCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGtttt                                                                                                                                     <  7:136862/148‑1 (MQ=255) ggCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTCCCAAGCTTTAGttgag                                                                                                                                    <  4:184418/149‑4 (MQ=255)    cAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCa                                                                                                                                 <  3:355264/149‑1 (MQ=255)      ggTCAAGGCAGGATGAAAAACGAAAAAAGGAGACAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCAc                                                                                                                                >  6:319297/1‑148 (MQ=255)       gTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTCCCAAGCTTTAGt                                                                                                                                        >  4:161016/1‑139 (MQ=255)       gTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTCCCAAGCTTTAGt                                                                                                                                        <  3:161016/139‑1 (MQ=255)          aaGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCGTGGGAAGCTTTCGTTTTGCCAcgtaac                                                                                                                           >  4:234043/1‑144 (MQ=255)               aGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGACCCCTCAACTAAAGCTTGGGACGCTTTCGTTGTGCGACTAAACTAGAc                                                                                                                      >  4:68552/1‑149 (MQ=255)                  aTGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCg                                                                                                                   >  5:302602/1‑149 (MQ=255)                       aaaCGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCt                                                                                                              >  7:282372/1‑149 (MQ=255)                         aCGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGa                                                                                                            <  6:302602/149‑1 (MQ=255)                           gAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGATTCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGc                                                                                                          >  8:332876/1‑149 (MQ=255)                           gAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGc                                                                                                          <  8:328164/149‑1 (MQ=255)                              aaaaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTc                                                                                                        >  3:263033/1‑148 (MQ=255)                              aaaaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTc                                                                                                        >  8:11614/1‑148 (MQ=255)                                aaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGAc                                                                                                     >  7:274751/1‑149 (MQ=255)                                aaGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGAc                                                                                                     >  7:90076/1‑149 (MQ=255)                                     gCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACttttt                                                                                                <  5:319297/149‑1 (MQ=255)                                           gCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGc                                                                                                                                   >  5:38377/1‑108 (MQ=255)                                           gCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGc                                                                                                                                   <  6:38377/108‑1 (MQ=255)                                             tCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAAc                                                                                        >  4:20552/1‑149 (MQ=255)                                                  ttGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCtt                                                                                     <  2:74875/147‑1 (MQ=255)                                                       tGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAAACCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCACATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGaa                                                                              >  1:294315/1‑149 (MQ=255)                                                       tGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAAACCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCACATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGaa                                                                              >  1:289739/1‑149 (MQ=255)                                                         cGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGaa                                                                              <  7:11614/147‑1 (MQ=255)                                                         cGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAAt                                                                             >  3:353228/1‑148 (MQ=255)                                                          gATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTa                                                                                               >  2:85363/1‑129 (MQ=255)                                                          gATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTa                                                                                               <  1:85363/129‑1 (MQ=255)                                                              aCGTTGCTGGAACGCCATTCGGGACTGCGTTGTGGAGCGGGTAGAGGTAATCGAACCCTCAACTAAAGGTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGaa                                                                        <  5:236522/148‑1 (MQ=255)                                                                    cTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAg                                                                 >  4:65210/1‑149 (MQ=255)                                                                    cTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATAACCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGAGAATTAGAAGCCCAAg                                                                 >  8:197849/1‑149 (MQ=255)                                                                     tGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCACGACTTTTTACCAGAACCTTCAGCGAATTAGACGCCCAAGt                                                                >  2:102770/1‑149 (MQ=255)                                                                       gAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTta                                                              >  3:94410/1‑149 (MQ=255)                                                                              aTGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATaaaaaa                                                       <  3:77379/149‑1 (MQ=255)                                                                                      cTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCg                                               >  5:217277/1‑149 (MQ=255)                                                                                       tGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCgg                                              >  7:47160/1‑149 (MQ=255)                                                                                         cGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGgc                                             <  4:353228/148‑1 (MQ=255)                                                                                                gAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTg                                     <  3:234043/149‑1 (MQ=255)                                                                                                     ggTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTg                                >  1:184960/1‑149 (MQ=255)                                                                                                         gagGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCgg                             <  8:274751/148‑1 (MQ=255)                                                                                                         gagGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCgg                             <  7:332876/148‑1 (MQ=255)                                                                                                           gggAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTa                          <  3:20552/149‑1 (MQ=255)                                                                                                            ggAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCg                              <  1:288180/144‑1 (MQ=255)                                                                                                            ggAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCg                              >  2:288180/1‑144 (MQ=255)                                                                                                                 cGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAACCCCAAGTTATAAAAAAGCGTGTCGGCGCCCCGTGGCCTGCGGGTATTGGCt                    <  8:282372/149‑1 (MQ=255)                                                                                                                     cccTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTATTgg                      >  6:85600/1‑143 (MQ=255)                                                                                                                     cccTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTATTgg                      <  5:85600/143‑1 (MQ=255)                                                                                                                     cccTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTATTGGCTACaa                <  2:184960/149‑1 (MQ=255)                                                                                                                        tCAACTAAAGATAGGGAAGCTTTCGGTGTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCAGGGGCCTGCGGGTATAGCCTACAAGgc             >  6:177474/1‑149 (MQ=255)                                                                                                                          aaCGAAAGCTTCCCAAGCTTTAGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTATTGGCTACAAGgcgc           >  3:180253/1‑149 (MQ=255)                                                                                                                                  cgtGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTATTGGCTACAAgg              <  1:312288/136‑1 (MQ=255)                                                                                                                                  cTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTATTGGCTACAAgg              >  2:312288/1‑138 (MQ=255)                                                                                                                                  cTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCACATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTATTGGCTACAAGGCGCCGAGCCGc   <  2:294315/149‑1 (MQ=255)                                                                                                                                  cTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCACATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTATTGGCTACAAGGCGCCGAGCCGc   <  2:289739/149‑1 (MQ=255)                                                                                                                                       gAAGCTTTCGTTTTGCCAATAAACTATACCCGCATCTGAGCTAGACTTTTTACCAGAACCTTCAGCGAATTACAATCCCAAGTTATAAAAAAGCTTGTCGGCTCCCCGTGGCCTGCGGGTATTGCCTACAAGGCGCCGAGCAGCt  >  6:273949/1‑145 (MQ=255)                                                                                                                                       |                                                                                                                                                 GGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACTTTTTACCAGAACCTTCAGCGAATTAGAAGCCCAAGTTATAAAAAAGCTTGTCGGCGCCCCGTGGCCTGCGGGTATTGGCTACAAGGCGCCGAGCCGCT  >  NC_002947/1606368‑1606646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑