Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,625,207 G→T 15.6% intergenic (+30/+27) PP_1425 → / ← nadB hypothetical protein/L‑aspartate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,625,2070GT15.6% 77.7 / 10.6 39intergenic (+30/+27)PP_1425/nadBhypothetical protein/L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base G (17/15);  new base T (4/2);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.61e-01

TGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTT  >  NC_002947/1625065‑1625355
                                                                                                                                              |                                                                                                                                                    
tGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCcg                                                                                                                                                >  2:99412/1‑149 (MQ=255)
tGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGACGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACAGGGTTAGCTTCTTCGCGGGCGAGCCcg                                                                                                                                                >  3:195381/1‑149 (MQ=255)
  ggggAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGGCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCcg                                                                                                                                                <  4:195381/147‑1 (MQ=255)
      aaaGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCTCGCCCGCGAAga                                                                                                                                          <  3:357432/149‑2 (MQ=255)
                    ccTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGGGGTTGAAGTTCAGCCGGCCGCGCTGGGCGGCGGTTAGCCGGGCATAGATCGACACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGata                                                                                                                            <  2:254477/149‑1 (MQ=255)
                    ccTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCACTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCTCGCCCGCGAAgaa                                                                                                                                         >  5:187152/1‑134 (MQ=255)
                    ccTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCACTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCTCGCCCGCGAAgaa                                                                                                                                         <  6:187152/136‑3 (MQ=255)
                      tCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGatat                                                                                                                           >  3:86837/1‑148 (MQ=255)
                                 tAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGtt                                                                                                                >  5:281843/1‑148 (MQ=255)
                                   ggggCGGACGAGCAGGGGCGCTGGCGGGGGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTaa                                                                                                              <  3:325267/148‑1 (MQ=255)
                                    gggCTGACGTGCAGGGGCGCTTGCGGGTGCGGTTGAAGTTCAGCGGGCGGCGCTGGGCTGGGGTGAGCCGGGAATTGATCGAGAGCGGGTTTGCTTCTTCGCGGGCGAGCCCGGGAAGGCCACGACGCCGATATCAGAGCGGGTTAAgg                                                                                                            <  1:99412/149‑1 (MQ=255)
                                      gCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGAt                                                                                                          >  3:332675/1‑149 (MQ=255)
                                       cTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATg                                                                                                         <  4:86837/149‑1 (MQ=255)
                                          aCGAGCATCGGCGCTTGCGGGGGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGgt                                                                                                       <  3:238538/148‑1 (MQ=255)
                                                 tCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTgg                                                                                               >  6:75066/1‑149 (MQ=255)
                                                        ttGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCt                                                                                                  >  8:197707/1‑139 (MQ=255)
                                                        ttGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCt                                                                                                  <  7:197707/139‑1 (MQ=255)
                                                          gCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGTGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTc                                                                                        <  5:147816/147‑1 (MQ=255)
                                                            gggTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGGCTCGTCCAg                                                                                     >  4:272112/1‑148 (MQ=255)
                                                                  ggTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCt                                                                              >  5:217665/1‑149 (MQ=255)
                                                                              gCCGGCGGCGCTGGGCGGCGGTGAGCCGGCCATAGAGCCAGACCGGGTTAGCTTCTGCGCGGCCCAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgt                                                                  <  2:42472/149‑1 (MQ=255)
                                                                               ccGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgtg                                                                 <  5:75066/149‑1 (MQ=255)
                                                                                   cggcgCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGTTTATCTTCTTCGCGGCGGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCCTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTa                                                             <  1:19244/149‑1 (MQ=255)
                                                                                     gcgcTGGGCTGCGGGGAGCCGGGCATAGCTCGAGACCGGGTTAGCTTCTGCGCGGGCGACCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAAt                                                           <  6:217665/149‑1 (MQ=255)
                                                                                         tGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgt                                                                  <  4:277576/138‑1 (MQ=255)
                                                                                         tGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgt                                                                  >  3:277576/1‑138 (MQ=255)
                                                                                                cGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCgg                                                >  2:248703/1‑149 (MQ=255)
                                                                                                        cGGGCATCTATCGAGCCCGGGTTAGCGTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCtt                                        <  4:136898/149‑1 (MQ=255)
                                                                                                                  tCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATg                              >  4:65600/1‑149 (MQ=255)
                                                                                                                           ggTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGGCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAg                      >  5:278605/1‑148 (MQ=255)
                                                                                                                              tAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGa                         >  6:359953/1‑142 (MQ=255)
                                                                                                                              tAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGa                         <  5:359953/142‑1 (MQ=255)
                                                                                                                                  ttcttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatcat                >  3:296857/1‑147 (MQ=255)
                                                                                                                                    cttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGc            >  6:154555/1‑149 (MQ=255)
                                                                                                                                    cttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATAAGAGCGGGTTAAGGATGGTGTCATTGGCCTAGTCCAGCATCCCTGGATAATCCAGTCTGTAATGAAACCAACGGCTATACATGCGATGCATGGCTGACAGGATCATAAg             >  4:168298/1‑148 (MQ=255)
                                                                                                                                    cttcGCGGGCGCGCCCGCGAAGGCCACGCCGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGc            >  7:252035/1‑149 (MQ=255)
                                                                                                                                     ttcGCGGGCTCGCCCGCGAAGGCCACGCCGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCt           >  5:194898/1‑149 (MQ=255)
                                                                                                                                     ttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACa                       <  2:160597/137‑1 (MQ=255)
                                                                                                                                     ttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACa                       >  1:160597/1‑137 (MQ=255)
                                                                                                                                              gAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACtt  >  7:204117/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
TGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTT  >  NC_002947/1625065‑1625355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑