Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,632,093	C→T	7.9%	P283S (CCT→TCT) ‡	lepA →	elongation factor 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,632,093	0	C	T	7.9%	218.7 / 6.9	76	P283S (CCT→TCT) ‡	lepA	elongation factor 4
Reads supporting (aligned to +/- strand):  ref base C (35/35);  new base T (4/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCACTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGG  >  NC_002947/1631946‑1632240                                                                                                                                                    |                                                                                                                                                     gTGCACTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGAcccc                                                                                                                                                     >  5:77379/1‑149 (MQ=255)    cACTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGa                                                                                                                                                  <  4:133576/149‑1 (MQ=255)     aCTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAg                                                                                                                                                 >  1:183005/1‑149 (MQ=255)     aCTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAg                                                                                                                                                 >  3:261232/1‑149 (MQ=255)          gTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGa                                                                                                                                           <  2:352647/149‑1 (MQ=255)             gACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGT‑CGAgga                                                                                                                                        >  6:106800/1‑148 (MQ=255)              aCAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGgtg                                                                                                                                       <  6:77379/149‑1 (MQ=255)               cAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGT‑CGAggaca                                                                                                                                      <  4:292393/149‑4 (MQ=255)                          tCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCTCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGT‑CGAggacagg                                                                                                                                    <  4:239795/140‑6 (MQ=255)                          tCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGT‑CGAggacagg                                                                                                                                    >  3:239795/1‑135 (MQ=255)                          tCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCtt                                                                                                                            <  4:117120/148‑1 (MQ=255)                            ttCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGCCCCTCTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCa                                                                                                                          >  3:45793/1‑148 (MQ=255)                            ttCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTc                                                                                                                           >  4:298496/1‑147 (MQ=255)                               aCCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCaagaa                                                                                                                      >  2:134742/1‑149 (MQ=255)                               aCCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCaaga                                                                                                                       <  3:301711/148‑1 (MQ=255)                                 cccGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCaag                                                                                                                        >  7:62695/1‑145 (MQ=255)                                 cccGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAagaaga                                                                                                                    <  3:81035/149‑1 (MQ=255)                                 cccGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAagaaga                                                                                                                    <  3:63166/149‑1 (MQ=255)                                   cGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAg                                                                                                                                                 <  8:304641/119‑1 (MQ=255)                                   cGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAg                                                                                                                                                 >  7:304641/1‑119 (MQ=255)                                           acCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGCCCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCg                                                                                                            >  2:295687/1‑147 (MQ=255)                                            cccAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGATGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAg                                                                                                         <  3:30798/149‑1 (MQ=255)                                              cAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGGATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGAGCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGt                                                                                                       <  1:6117/149‑1 (MQ=255)                                                   cGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACg                                                                                                  >  8:248317/1‑149 (MQ=255)                                                    gCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGc                                                                                                 >  6:183352/1‑149 (MQ=255)                                                      tGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCg                                                                                               >  5:100164/1‑149 (MQ=255)                                                            gAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGt                                                                                         >  4:26269/1‑149 (MQ=255)                                                            gAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGt                                                                                         >  5:146622/1‑149 (MQ=255)                                                             aaaGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGcc                                                                                            <  4:261232/145‑1 (MQ=255)                                                             aaaGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGtt                                                                                        >  1:47757/1‑149 (MQ=255)                                                                gacGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCT‑‑ACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCgg                                                                                   >  5:53235/3‑149 (MQ=255)                                                                gCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTccc                                                                                     <  3:26269/149‑1 (MQ=255)                                                                         gTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTc                                                                            <  1:134742/149‑1 (MQ=255)                                                                         gTAGGCTTCATCACCGCCACCATCAAGGACACCCACGGTGCCCCGGTTGGTCACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAACATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGGCAGccc                                                                            <  2:320083/149‑3 (MQ=255)                                                                           aGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGAt                                                                                                                   >  4:220691/1‑108 (MQ=255)                                                                           aGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGAt                                                                                                                   <  3:220691/108‑1 (MQ=255)                                                                           aGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTcc                                                                           >  1:364189/1‑148 (MQ=255)                                                                            ggCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCcg                                                                          <  3:298496/148‑1 (MQ=255)                                                                             gCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTccc                                                                                     <  5:257710/136‑1 (MQ=255)                                                                             gCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTccc                                                                                     >  6:257710/1‑136 (MQ=255)                                                                              cTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCcgac                                                                        >  6:228794/1‑148 (MQ=255)                                                                                tcatcaTCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCgacgac                                                                     >  7:193220/1‑149 (MQ=255)                                                                                   tcatcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCg                                                                                                            >  5:112196/1‑107 (MQ=255)                                                                                   tcatcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCg                                                                                                            <  6:112196/107‑1 (MQ=255)                                                                                   tcatcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCT‑‑ACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                <  6:53235/149‑1 (MQ=255)                                                                                     atcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                <  8:62695/149‑1 (MQ=255)                                                                                     atcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                <  2:183005/149‑1 (MQ=255)                                                                                      tcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAg                                                               >  7:306153/1‑149 (MQ=255)                                                                                             cATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTGTTGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTcc                                                        <  1:295687/149‑1 (MQ=255)                                                                                             cATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTcc                                                        <  5:228794/149‑1 (MQ=255)                                                                                               tCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCcgc                                                      >  1:204097/1‑149 (MQ=255)                                                                                                         ccACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTg                                             >  7:257330/1‑148 (MQ=255)                                                                                                         ccACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTg                                             >  1:254000/1‑148 (MQ=255)                                                                                                             ggTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGaa                                        <  6:100164/149‑1 (MQ=255)                                                                                                              gTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTGCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAg                                          >  1:82335/1‑147 (MQ=255)                                                                                                               tGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGc                                      <  5:183352/149‑1 (MQ=255)                                                                                                                  cccGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGa                                   <  2:82335/149‑1 (MQ=255)                                                                                                                   ccGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGAc                                  >  3:116013/1‑149 (MQ=255)                                                                                                                    cGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGAcc                                 <  7:248317/149‑1 (MQ=255)                                                                                                                     ggTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGAccc                                <  4:116013/149‑1 (MQ=255)                                                                                                                          gTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGaa                            <  8:306153/148‑1 (MQ=255)                                                                                                                          gTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAAc                           <  7:245731/149‑1 (MQ=255)                                                                                                                              cacCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGAct                       <  8:257330/149‑1 (MQ=255)                                                                                                                              cacCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGAct                       <  2:254000/149‑1 (MQ=255)                                                                                                                               accctgaccctgTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGGCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                >  1:118010/1‑107 (MQ=255)                                                                                                                               accctgaccctgTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                <  2:118010/107‑1 (MQ=255)                                                                                                                               accctgaccctgTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGaa                            >  6:102692/1‑143 (MQ=255)                                                                                                                               accctgaccctgTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGaa                            <  5:102692/143‑1 (MQ=255)                                                                                                                               accctgaccctgTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActc                      >  8:270004/1‑149 (MQ=255)                                                                                                                                        ctgTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTg               >  3:58751/1‑147 (MQ=255)                                                                                                                                           accTCGACCTCAGGGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTa          >  2:192390/2‑149 (MQ=255)                                                                                                                                             cTCGACCTCAGGGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACa        >  3:344556/1‑149 (MQ=255)                                                                                                                                             cTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACa        >  2:75756/1‑149 (MQ=255)                                                                                                                                             cTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACa        >  6:47976/1‑149 (MQ=255)                                                                                                                                             cTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACa        >  2:189029/1‑149 (MQ=255)                                                                                                                                             cTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTACGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTAc         <  4:45793/148‑1 (MQ=255)                                                                                                                                              tCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACAt       >  2:18079/1‑149 (MQ=255)                                                                                                                                                   cccTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGc     <  2:272214/146‑1 (MQ=255)                                                                                                                                                   cccTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGc     >  1:272214/1‑146 (MQ=255)                                                                                                                                                   cccTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCg   >  7:294638/1‑148 (MQ=255)                                                                                                                                                   cccTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCg   >  4:198653/1‑148 (MQ=255)                                                                                                                                                    ccTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCgg  >  4:355558/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GTGCACTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGT‑CGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGG  >  NC_002947/1631946‑1632240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑