Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,633,554	A→T	6.0%	Q168L (CAG→CTG)	lepB →	signal peptidase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,633,554	0	A	T	6.0%	249.4 / 4.5	83	Q168L (CAG→CTG)	lepB	signal peptidase I
Reads supporting (aligned to +/- strand):  ref base A (41/37);  new base T (2/3);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

ATCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGC  >  NC_002947/1633409‑1633700                                                                                                                                                   |                                                                                                                                                   aTCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGcc                                                                                                                                                 >  1:140669/1‑149 (MQ=255) aTCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGcc                                                                                                                                                 >  2:56841/1‑149 (MQ=255)    gAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGt                                                                                                                                              <  3:141452/149‑1 (MQ=255)                ccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACt                                                                                                                                  >  3:306416/1‑149 (MQ=255)                ccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACt                                                                                                                                  >  1:240220/1‑149 (MQ=255)                ccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACt                                                                                                                                  >  1:245381/1‑149 (MQ=255)                ccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACt                                                                                                                                  >  3:101586/1‑149 (MQ=255)                  gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtgg                                                                                                                                >  1:22583/1‑149 (MQ=255)                  gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaacagccgc                                                                                                                                <  7:342420/149‑6 (MQ=39)                   cAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggt                                                                                                                               <  4:101586/149‑1 (MQ=255)                        cgGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCa                                                                                                                          >  8:126435/1‑149 (MQ=255)                         ggggATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGTCAGCCCGTTGCCGAACAACTGGTGGGCAg                                                                                                                         >  4:233025/1‑149 (MQ=255)                            gATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGa                                                                                                                      <  2:240220/149‑1 (MQ=255)                            gATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGa                                                                                                                      <  3:85041/149‑1 (MQ=255)                            gATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGGGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGa                                                                                                                      <  2:245381/149‑1 (MQ=255)                              tGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGc                                                                                                                    <  4:193426/149‑1 (MQ=255)                              tGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGAGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGc                                                                                                                    <  1:302541/149‑1 (MQ=255)                                tAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGcc                                                                                                                   <  2:344048/148‑1 (MQ=255)                                 aaTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGcc                                                                                                                   <  1:121738/147‑1 (MQ=255)                                 aaTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGcc                                                                                                                   >  2:121738/1‑147 (MQ=255)                                 aaTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCgg                                                                                                                 >  8:14529/1‑149 (MQ=255)                                  aTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCggg                                                                                                                >  5:52990/1‑149 (MQ=255)                                  aTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCggg                                                                                                                >  7:201805/1‑149 (MQ=255)                                  aTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCgg                                                                                                                 <  1:56841/148‑1 (MQ=255)                                        ttCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCt                                                                                                          <  4:306416/149‑1 (MQ=255)                                            gCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGc                                                                                                      <  2:140669/149‑1 (MQ=255)                                             cTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCa                                                                                                     <  2:68927/149‑1 (MQ=255)                                             cTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaac                                                                                                                                      <  7:321278/116‑1 (MQ=39)                                             cTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaac                                                                                                                                      >  8:321278/1‑116 (MQ=39)                                              tACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTggg                                                                                                       >  7:95413/1‑146 (MQ=255)                                                cccGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCa                                                                                                     <  8:95413/146‑1 (MQ=255)                                                cccGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTg                                                                                                  <  8:325505/149‑1 (MQ=255)                                                cccGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTg                                                                                                  <  7:126435/149‑1 (MQ=255)                                                   gAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCgg                                                                                                                 >  8:313382/1‑131 (MQ=255)                                                   gAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCgg                                                                                                                 <  7:313382/131‑1 (MQ=255)                                                    aGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCa                                                                                              <  2:22583/149‑1 (MQ=255)                                                     gTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAg                                                                                             >  4:267220/1‑149 (MQ=255)                                                        aCCCTAATGTCACCTAGCATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCt                                                                                           <  1:127895/149‑1 (MQ=255)                                                         cccGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTg                                                                                          >  5:66712/1‑148 (MQ=255)                                                          ccGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGt                                                                                         >  3:279318/1‑148 (MQ=255)                                                          ccGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTa                                                                                        <  3:299619/149‑1 (MQ=255)                                                               tGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAgg                                                                                   <  6:52990/149‑1 (MQ=255)                                                                       a‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTc                                                                           >  1:168559/1‑149 (MQ=255)                                                                         cATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCg                                                                          <  4:279318/149‑1 (MQ=255)                                                                          aTCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCgg                                                                         <  3:267220/149‑1 (MQ=255)                                                                           tCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGgc                                                                        >  3:277644/1‑149 (MQ=255)                                                                           tCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaac                                                                                                                                      <  2:368695/87‑1 (MQ=37)                                                                           tCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaac                                                                                                                                      >  1:368695/1‑87 (MQ=37)                                                                            cAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGt                                                                                                   >  8:29293/1‑121 (MQ=255)                                                                            cAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGt                                                                                                   <  7:29293/121‑1 (MQ=255)                                                                            cAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGgcg                                                                       >  7:227331/1‑149 (MQ=255)                                                                                 gtgtGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGcc                                                                  >  8:344047/1‑149 (MQ=255)                                                                                     ggTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCGGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAac                                                              <  8:30583/149‑1 (MQ=255)                                                                                      gTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGcc                                                                                                                   >  3:267448/1‑95 (MQ=255)                                                                                      gTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGcc                                                                                                                   <  4:267448/95‑1 (MQ=255)                                                                                       ttGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAacac                                                            <  7:344047/149‑1 (MQ=255)                                                                                       ttGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAaca                                                             >  4:59438/1‑148 (MQ=255)                                                                                         ggCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACAcc                                                           <  3:49930/148‑1 (MQ=255)                                                                                            cTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTggg                                                                                                       >  7:107060/1‑101 (MQ=255)                                                                                                     gACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAgg                                               >  3:149082/1‑148 (MQ=255)                                                                                                      aCGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTCCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGa                                              >  1:300806/1‑148 (MQ=255)                                                                                                            tCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGc                                       >  3:198571/1‑149 (MQ=255)                                                                                                              cGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATCAGCCg                                     >  7:61786/1‑149 (MQ=255)                                                                                                                 tACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACAGGTTGGCAGAGATAAAGGTAACATTGGAAGAGCTCAGATGTAAAAAGAAAAGCTCGTCGCGGCCGAACACCTGATCCTCAAGGAAATGAGACGTTa                                  >  2:308811/1‑149 (MQ=255)                                                                                                                     ccAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGc                              <  2:95963/149‑1 (MQ=255)                                                                                                                      cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAAt                                           <  4:310491/135‑1 (MQ=255)                                                                                                                      cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAAt                                           >  3:310491/1‑135 (MQ=255)                                                                                                                      cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCa                             >  1:154821/1‑149 (MQ=255)                                                                                                                      cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCa                             >  7:196092/1‑149 (MQ=255)                                                                                                                      cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGATATCGCa                             >  4:352126/1‑149 (MQ=255)                                                                                                                       aaCGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCTCAAGGAAATGAGCCGTTATCGCAt                            <  8:227331/149‑1 (MQ=255)                                                                                                                          gACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATTCGCAAGGAAATGAGCCGTTATCGCATgcc                         >  1:53223/1‑149 (MQ=255)                                                                                                                            cAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATgccgc                       <  4:149082/149‑1 (MQ=255)                                                                                                                               gCGGCTGTTCGTCAACGGTCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCgg                    <  3:233025/149‑1 (MQ=255)                                                                                                                                      ttCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACcagca             <  4:123348/149‑1 (MQ=255)                                                                                                                                         gTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg          <  8:201805/149‑1 (MQ=255)                                                                                                                                          tCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg          >  4:17346/1‑148 (MQ=255)                                                                                                                                            aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg          >  1:351674/1‑146 (MQ=255)                                                                                                                                            aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg          <  2:351674/146‑1 (MQ=255)                                                                                                                                            aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGAc       >  4:194737/1‑149 (MQ=255)                                                                                                                                            aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGAc       >  5:274208/1‑149 (MQ=255)                                                                                                                                                 ccAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGc  >  8:52037/1‑149 (MQ=255)                                                                                                                                                 ccAGCCCGTTGCCGAACAACTGGGGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGc  <  2:199136/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   ATCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGC  >  NC_002947/1633409‑1633700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑