Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,633,563	C→A	6.0%	A171D (GCC→GAC)	lepB →	signal peptidase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,633,563	0	C	A	6.0%	271.8 / 6.0	84	A171D (GCC→GAC)	lepB	signal peptidase I
Reads supporting (aligned to +/- strand):  ref base C (39/40);  new base A (2/3);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.14e-01

CCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCGGGCC  >  NC_002947/1633424‑1633709                                                                                                                                             |                                                                                                                                                   ccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACt                                                                                                                                           >  3:306416/1‑149 (MQ=255) ccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACt                                                                                                                                           >  1:240220/1‑149 (MQ=255) ccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACt                                                                                                                                           >  1:245381/1‑149 (MQ=255) ccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACt                                                                                                                                           >  3:101586/1‑149 (MQ=255)   gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtgg                                                                                                                                         >  1:22583/1‑149 (MQ=255)   gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaacagccgc                                                                                                                                         <  7:342420/149‑6 (MQ=39)    cAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggt                                                                                                                                        <  4:101586/149‑1 (MQ=255)         cgGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCa                                                                                                                                   >  8:126435/1‑149 (MQ=255)          ggggATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGTCAGCCCGTTGCCGAACAACTGGTGGGCAg                                                                                                                                  >  4:233025/1‑149 (MQ=255)             gATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGa                                                                                                                               <  2:240220/149‑1 (MQ=255)             gATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGa                                                                                                                               <  3:85041/149‑1 (MQ=255)             gATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGGGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGa                                                                                                                               <  2:245381/149‑1 (MQ=255)               tGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGc                                                                                                                             <  4:193426/149‑1 (MQ=255)               tGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGAGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGc                                                                                                                             <  1:302541/149‑1 (MQ=255)                 tAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGcc                                                                                                                            <  2:344048/148‑1 (MQ=255)                  aaTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGcc                                                                                                                            <  1:121738/147‑1 (MQ=255)                  aaTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGcc                                                                                                                            >  2:121738/1‑147 (MQ=255)                  aaTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCgg                                                                                                                          >  8:14529/1‑149 (MQ=255)                   aTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCggg                                                                                                                         >  7:201805/1‑149 (MQ=255)                   aTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCggg                                                                                                                         >  5:52990/1‑149 (MQ=255)                   aTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCgg                                                                                                                          <  1:56841/148‑1 (MQ=255)                         ttCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCt                                                                                                                   <  4:306416/149‑1 (MQ=255)                             gCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGc                                                                                                               <  2:140669/149‑1 (MQ=255)                              cTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCa                                                                                                              <  2:68927/149‑1 (MQ=255)                              cTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaac                                                                                                                                               <  7:321278/116‑1 (MQ=39)                              cTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaac                                                                                                                                               >  8:321278/1‑116 (MQ=39)                               tACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTggg                                                                                                                >  7:95413/1‑146 (MQ=255)                                 cccGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCa                                                                                                              <  8:95413/146‑1 (MQ=255)                                 cccGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTg                                                                                                           <  7:126435/149‑1 (MQ=255)                                 cccGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTg                                                                                                           <  8:325505/149‑1 (MQ=255)                                    gAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCgg                                                                                                                          >  8:313382/1‑131 (MQ=255)                                    gAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCgg                                                                                                                          <  7:313382/131‑1 (MQ=255)                                     aGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCa                                                                                                       <  2:22583/149‑1 (MQ=255)                                      gTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAg                                                                                                      >  4:267220/1‑149 (MQ=255)                                         aCCCTAATGTCACCTAGCATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCt                                                                                                    <  1:127895/149‑1 (MQ=255)                                          cccGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTg                                                                                                   >  5:66712/1‑148 (MQ=255)                                           ccGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGt                                                                                                  >  3:279318/1‑148 (MQ=255)                                           ccGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTa                                                                                                 <  3:299619/149‑1 (MQ=255)                                                tGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAgg                                                                                            <  6:52990/149‑1 (MQ=255)                                                        a‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTc                                                                                    >  1:168559/1‑149 (MQ=255)                                                          cATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCg                                                                                   <  4:279318/149‑1 (MQ=255)                                                           aTCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCgg                                                                                  <  3:267220/149‑1 (MQ=255)                                                            tCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGgc                                                                                 >  3:277644/1‑149 (MQ=255)                                                            tCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaac                                                                                                                                               >  1:368695/1‑87 (MQ=37)                                                            tCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaac                                                                                                                                               <  2:368695/87‑1 (MQ=37)                                                             cAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGt                                                                                                            >  8:29293/1‑121 (MQ=255)                                                             cAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGt                                                                                                            <  7:29293/121‑1 (MQ=255)                                                             cAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGgcg                                                                                >  7:227331/1‑149 (MQ=255)                                                                  gtgtGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGcc                                                                           >  8:344047/1‑149 (MQ=255)                                                                      ggTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCGGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAac                                                                       <  8:30583/149‑1 (MQ=255)                                                                       gTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGcc                                                                                                                            >  3:267448/1‑95 (MQ=255)                                                                       gTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGcc                                                                                                                            <  4:267448/95‑1 (MQ=255)                                                                        ttGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAacac                                                                     <  7:344047/149‑1 (MQ=255)                                                                        ttGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAaca                                                                      >  4:59438/1‑148 (MQ=255)                                                                          ggCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACAcc                                                                    <  3:49930/148‑1 (MQ=255)                                                                             cTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTggg                                                                                                                >  7:107060/1‑101 (MQ=255)                                                                                      gACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAgg                                                        >  3:149082/1‑148 (MQ=255)                                                                                       aCGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTCCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGa                                                       >  1:300806/1‑148 (MQ=255)                                                                                             tCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGc                                                >  3:198571/1‑149 (MQ=255)                                                                                               cGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATCAGCCg                                              >  7:61786/1‑149 (MQ=255)                                                                                                  tACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACAGGTTGGCAGAGATAAAGGTAACATTGGAAGAGCTCAGATGTAAAAAGAAAAGCTCGTCGCGGCCGAACACCTGATCCTCAAGGAAATGAGACGTTa                                           >  2:308811/1‑149 (MQ=255)                                                                                                      ccAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGc                                       <  2:95963/149‑1 (MQ=255)                                                                                                       cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAAt                                                    <  4:310491/135‑1 (MQ=255)                                                                                                       cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAAt                                                    >  3:310491/1‑135 (MQ=255)                                                                                                       cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCa                                      >  7:196092/1‑149 (MQ=255)                                                                                                       cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCa                                      >  1:154821/1‑149 (MQ=255)                                                                                                       cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGATATCGCa                                      >  4:352126/1‑149 (MQ=255)                                                                                                        aaCGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCTCAAGGAAATGAGCCGTTATCGCAt                                     <  8:227331/149‑1 (MQ=255)                                                                                                           gACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATTCGCAAGGAAATGAGCCGTTATCGCATgcc                                  >  1:53223/1‑149 (MQ=255)                                                                                                             cAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATgccgc                                <  4:149082/149‑1 (MQ=255)                                                                                                                gCGGCTGTTCGTCAACGGTCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCgg                             <  3:233025/149‑1 (MQ=255)                                                                                                                       ttCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACcagca                      <  4:123348/149‑1 (MQ=255)                                                                                                                          gTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg                   <  8:201805/149‑1 (MQ=255)                                                                                                                           tCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg                   >  4:17346/1‑148 (MQ=255)                                                                                                                             aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg                   <  2:351674/146‑1 (MQ=255)                                                                                                                             aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg                   >  1:351674/1‑146 (MQ=255)                                                                                                                             aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGAc                >  5:274208/1‑149 (MQ=255)                                                                                                                             aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGAc                >  4:194737/1‑149 (MQ=255)                                                                                                                                  ccAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGc           >  8:52037/1‑149 (MQ=255)                                                                                                                                  ccAGCCCGTTGCCGAACAACTGGGGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGc           <  2:199136/149‑1 (MQ=255)                                                                                                                                        cggagCCCAACAACTGGTGGCCAGCGAGCCGGGTACCCTCGGCAGTGCTCAGCTGAACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCgg     <  4:277644/145‑1 (MQ=255)                                                                                                                                        cGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCgg     <  3:226996/149‑1 (MQ=255)                                                                                                                                         gTTGCCGAACATCTGGTGGGCAGCGAGCCGGTTAGCCTGGGCAGTGTTCAGCTGTACAAGGAAAAGCTCTGGGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCggg    <  8:61786/149‑1 (MQ=255)                                                                                                                                            gCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATTCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCGGGcc  <  2:53223/148‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                   CCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTA‑CATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCGGGCC  >  NC_002947/1633424‑1633709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑