Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,655,523 G→C 6.0% L416V (CTG→GTG) ‡ PP_1449 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,655,5230GC 6.0% 222.8 / 3.0 67L416V (CTG→GTG) ‡PP_1449hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/33);  new base C (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

CTTTATCGACATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGGGTGGTGGTGGTCTTGCGG  >  NC_002947/1655377‑1655671
                                                                                                                                                   |                                                                                                                                                    
cTTTATCGACATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGc                                                                                                                                                    >  6:228850/1‑149 (MQ=255)
cTTTATCGACATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGc                                                                                                                                                    >  6:354502/1‑149 (MQ=255)
cTTTATCGACATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGc                                                                                                                                                    >  7:137367/1‑149 (MQ=255)
 tttATCGACATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGcc                                                                                                                                                   >  7:349325/1‑149 (MQ=255)
 tttATCGACATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGcc                                                                                                                                                   >  7:180302/1‑149 (MQ=255)
        aCATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGTTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCg                                                                                                                                            <  7:59472/149‑1 (MQ=255)
          aTGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCCTGGACATCCTCGCCGGc                                                                                                                                                <  1:227171/143‑1 (MQ=255)
          aTGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCCTGGACATCCTCGCCGGc                                                                                                                                                >  2:227171/1‑143 (MQ=255)
              gCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTc                                                                                                                                       >  4:322799/1‑148 (MQ=255)
               cccGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGa                                                                                                                                     <  3:117242/149‑1 (MQ=255)
                ccGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGAc                                                                                                                                    >  4:354048/1‑149 (MQ=255)
                 cGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGAcc                                                                                                                                   <  5:228850/149‑1 (MQ=255)
                    cgcTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGGCGCTATCGCTGTCACCGCGCCATACATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTc                                                                                                                                <  4:324106/149‑1 (MQ=255)
                      cTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAg                                                                                                                              >  5:243934/1‑149 (MQ=255)
                         aGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCt                                                                                                                            <  8:180302/148‑1 (MQ=255)
                         aGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTc                                                                                                                           <  5:277381/149‑1 (MQ=255)
                         aGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTc                                                                                                                           <  3:322799/149‑1 (MQ=255)
                         aGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCGGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGCCCGGCACCGCTGTCGACCGTCAGCTc                                                                                                                           <  4:291008/149‑1 (MQ=255)
                               cTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTAc                                                                                                                      <  5:354502/148‑1 (MQ=255)
                                     tttGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGtt                                                                                                                        >  5:245960/1‑140 (MQ=255)
                                     tttGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGtt                                                                                                                        <  6:245960/140‑1 (MQ=255)
                                     tttGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCCTGGACATCCTCGCCGGCATCgaca                                                                                                                                         >  8:344062/1‑120 (MQ=38)
                                     tttGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCCTGGACATCCTCGCCGGCATCgaca                                                                                                                                         <  7:344062/123‑4 (MQ=38)
                                          ggTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCt                                                                                                          >  2:40278/1‑149 (MQ=255)
                                            tttCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTc                                                                                                        >  3:169326/1‑149 (MQ=255)
                                            tttCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGACGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTc                                                                                                        >  8:137849/1‑149 (MQ=255)
                                                attGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCCCCTTCgcgg                                                                                                    >  4:252368/2‑149 (MQ=255)
                                                 ttGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCgcggc                                                                                                   >  4:293891/1‑149 (MQ=255)
                                                 ttGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCgcggc                                                                                                   >  2:224805/1‑149 (MQ=255)
                                                         gTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATgc                                                                                           <  1:224805/149‑1 (MQ=255)
                                                            gCTATCGCTGGCACCGCGCCATAATTC‑CTTGCGATGACGCTCGTGCTGTTTGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATgcgc                                                                                         <  5:152533/148‑1 (MQ=255)
                                                             cTATCGCTGTCACCGCGCCATAAATCGCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCa                                                                                        >  1:192264/1‑149 (MQ=255)
                                                             cTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCAt                                                                                       <  5:48062/149‑1 (MQ=255)
                                                                tCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATg                                                                                      <  3:293891/147‑1 (MQ=255)
                                                                   cTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATgtcgtc                                                                                 <  5:153512/149‑1 (MQ=255)
                                                                   cTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATgtcgtc                                                                                 >  4:161201/1‑149 (MQ=255)
                                                                    tGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGtcgtcg                                                                                >  6:220458/1‑149 (MQ=255)
                                                                        aCCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCg                                                                             >  6:311776/1‑148 (MQ=255)
                                                                        aCCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCg                                                                             <  8:137367/148‑1 (MQ=255)
                                                                         ccGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGa                                                                           <  3:46938/149‑1 (MQ=255)
                                                                         ccGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGa                                                                           <  6:243934/149‑1 (MQ=255)
                                                                          cgcgCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGAc                                                                          >  6:258667/1‑149 (MQ=255)
                                                                               cATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCaa                                                                     <  3:354048/149‑1 (MQ=255)
                                                                                 tAAATC‑CTTGCGATGACGCACCTGCTGTGCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGTGTCCACCTTCGCGGCGGTCATGCGCATGGCGTCGCCGGACTGCAACa                                                                   <  1:267468/149‑1 (MQ=255)
                                                                                   aaTC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGc                                                                 <  3:161201/149‑1 (MQ=255)
                                                                                            cGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGctct                                                          <  5:311776/148‑1 (MQ=255)
                                                                                            cGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCtctc                                                         <  8:349325/149‑1 (MQ=255)
                                                                                             gATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCa                                                        <  7:204204/149‑1 (MQ=255)
                                                                                                    cACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCa                                                 <  2:81645/149‑1 (MQ=255)
                                                                                                      ccTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAg                                                >  3:242865/1‑148 (MQ=255)
                                                                                                         gctgTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAAt                                            >  6:374428/1‑149 (MQ=255)
                                                                                                         gctgTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAAt                                            >  2:34999/1‑149 (MQ=255)
                                                                                                                    cGTTTGCTGTCGATGCCGACGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCtg                                 <  7:137849/149‑1 (MQ=255)
                                                                                                                          cTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCTGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGttgttg                           <  5:258667/149‑1 (MQ=255)
                                                                                                                          cTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGttgttg                           <  1:182360/149‑1 (MQ=255)
                                                                                                                           tGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGc                          >  4:331939/1‑149 (MQ=255)
                                                                                                                               gATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTtgt                      <  4:169326/149‑1 (MQ=255)
                                                                                                                                aTGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTtgtg                     <  4:242865/149‑1 (MQ=255)
                                                                                                                                aTGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTtgt                      <  7:57594/148‑1 (MQ=255)
                                                                                                                                aTGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTtgt                      >  8:57594/1‑148 (MQ=255)
                                                                                                                                    cGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTgtgggtg                 >  6:351647/1‑149 (MQ=255)
                                                                                                                                     ggCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTgtgggtgg                >  2:315235/1‑149 (MQ=255)
                                                                                                                                       cGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGggtggtg              <  5:374428/149‑1 (MQ=255)
                                                                                                                                                 cAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCtg                                 >  4:147830/1‑120 (MQ=255)
                                                                                                                                                 cAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCtg                                 <  3:147830/120‑1 (MQ=255)
                                                                                                                                                 cAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGGGTGGTGGTGGTCTTGc    >  5:340856/1‑149 (MQ=255)
                                                                                                                                                  aGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGGGTGGTGGTGGTCTTGCg   >  3:175002/1‑149 (MQ=255)
                                                                                                                                                   ggCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGGGTGGTGGTGGTCTTGCgg  <  5:220458/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTTTATCGACATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACCGCGCCATAAATC‑CTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGGGTGGTGGTGGTCTTGCGG  >  NC_002947/1655377‑1655671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑