| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,703,250 | G→C | 22.0% | intergenic (+99/‑68) | lysS → / → PP_1497 | lysine‑‑tRNA ligase/TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,703,250 | 0 | G | C | 22.0% | 93.4 / 25.9 | 50 | intergenic (+99/‑68) | lysS/PP_1497 | lysine‑‑tRNA ligase/TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (22/17); new base C (6/5); total (28/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
GATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAA > NC_002947/1703106‑1703397 | gATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑tt < 2:37672/149‑1 (MQ=255) aTCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCC‑tttg < 5:298899/149‑3 (MQ=38) aTCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCC‑tttg < 2:229205/149‑3 (MQ=255) cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTTCTACAAAGGCC‑TTGTAg < 2:231281/149‑1 (MQ=255) gACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCTT‑TTGTAGGAg > 4:120242/1‑149 (MQ=255) aCGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGc < 7:71080/149‑1 (MQ=255) tCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCTT‑TTGTAGGAGCggg < 3:120242/149‑1 (MQ=255) aTCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGtt < 1:217925/149‑1 (MQ=255) cTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCTCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTa < 8:323693/148‑1 (MQ=255) ttCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑ccc < 6:380323/148‑1 (MQ=255) acaTGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAggg > 4:285298/1‑149 (MQ=255) gCGTCCGCAGGCCTGAGTGAGCTGAACGTGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCT‑TTGTAg > 5:361922/1‑123 (MQ=255) gCGTCCGCAGGCCTGAGTGAGCTGAACGTGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCT‑TTGTAg < 6:361922/123‑1 (MQ=255) agCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACAcccc > 7:197843/1‑148 (MQ=255) cTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCT‑TTGTAGGAGc < 8:207813/106‑1 (MQ=255) cTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCT‑TTGTAGGAGc > 7:207813/1‑106 (MQ=255) gAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTAGAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGa > 3:102280/1‑136 (MQ=255) accgcgccgccTTTCGCGGGGGATTTTTCTTGCCTTAAGCTTTATGTTGTCTGTCCCGGCCTCTTCGCGGTAAACCCCGCTCCTACAACGGCC‑TTTTTGGTGGGGTTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGa < 8:197843/143‑1 (MQ=255) aaCGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCCGCACTGACGAAACACCCCCATGAg > 8:8281/1‑149 (MQ=255) aGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTAt < 3:285298/149‑1 (MQ=255) ccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATccccc > 6:368468/1‑149 (MQ=255) tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCAt > 4:295860/1‑149 (MQ=255) tttCTTGCCTTAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAACGGCC‑TTTTAGGAGGGGTTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATg < 7:8281/148‑1 (MQ=38) cggAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑GTGTAGGAGCGGGTTTT‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCCTGAGGTATCCCCCGGGCCACCCGCCCAGCCTGGcc > 8:217977/3‑147 (MQ=25) tACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACCCCCCCCTGAGGTATCCCCCGTGACACCCGCCATGCCTGCCCAAGgc > 3:46255/1‑132 (MQ=255) ccGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCAccg > 7:271114/1‑149 (MQ=255) tctTCGCGGGTAAACCCGCTCCTACAAAGGCC‑GTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACCCCCCCATGAGGTATCCCCCGTGTCCCCCGCCATGCCTGCCCACGGCGCCGCCGGCCTCGCCACCGCCGTCg > 5:201984/1‑149 (MQ=37) tctTCGCGGGTAAACCCGCTCCTACAAAGGCC‑GTGTAGGAGCGGGGTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACCCCCCCATGAGGGATCCCCCGTGACACCCGCCATGCCGGCCCAAGGCGCCGCCGGCATCGCCCCCGCCGTCg > 3:296936/1‑149 (MQ=37) tctcgGTTAAACCCGCTCCTACAACGCCC‑TTGTAGGAGGGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGc < 2:294170/144‑1 (MQ=39) cgGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGa > 7:118549/1‑147 (MQ=255) gggTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGc > 1:294170/1‑143 (MQ=255) gggTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAg > 7:41004/1‑149 (MQ=255) cggTAAACCCCCCCCAACAACCGCC‑TTGTCCGCCGGGGTTTACCCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGaaa < 5:231269/148‑1 (MQ=255) aaaCCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGt < 4:154609/148‑1 (MQ=255) aaaCCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTg > 3:154609/1‑149 (MQ=255) aaCCCGCTCCTACAAAGGCC‑TTGTAGGAGCTGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGc < 5:368468/149‑1 (MQ=255) ctccGATCCTACAAGGCCT‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGACGGCATCGCCACCGCCGTCGCCGAAAGCGTGc < 7:326366/146‑1 (MQ=38) cccGCTCCTACAAAGGCC‑TTGTAGGCGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACCCCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGACAGCGTGCaa > 8:268350/1‑149 (MQ=255) cccGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCaa > 3:203735/1‑149 (MQ=255) ccGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCaa > 7:223175/1‑148 (MQ=255) ccGCTCCTACAAAGGCC‑TTGTAAGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAACGCGTGCaa > 7:56186/1‑148 (MQ=255) cGCTCCTACAAGCCAT‑TTCTAGGAGCGGGTTTA‑CCCGCTAAGGGGCCAGCACTGACGAAACACCAACAAGAGCTATCACCAGTCACACCCGCCATGCAACACCAACACGCCGACGGCAACGCCACCGCCGACCCCCAAAGCGTGCaaca > 8:326366/1‑147 (MQ=255) gCTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAc > 4:319008/1‑149 (MQ=255) cTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCGGTACTGACAAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAc > 5:247030/1‑148 (MQ=37) acTACAACGGCT‑TTGTCGGTGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCaa < 6:201984/148‑1 (MQ=255) tACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAgg > 6:35160/1‑149 (MQ=255) aaaGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCg > 7:254856/1‑149 (MQ=255) aaaGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCg < 8:41004/149‑1 (MQ=255) aaGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGCCCg > 8:88424/1‑148 (MQ=255) aGGCCTTTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGc < 3:319008/149‑1 (MQ=255) ggCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCaa < 7:217977/149‑1 (MQ=255) | GATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTA‑CCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAA > NC_002947/1703106‑1703397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |