Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,724,644 T→A 6.2% K175N (AAA→AAT)  PP_1518 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,724,6440TA 6.2% 218.7 / 2.5 74K175N (AAA→AAT) PP_1518hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base A (2/3);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

AGACCTTCTTGGCCAGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTGGGTGGCCGAGTCGTCACCGCCGCTGAG  >  NC_002947/1724496‑1724790
                                                                                                                                                    |                                                                                                                                                  
aGACCTTCTTGGCCAGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCt                                                                                                                                                    <  7:348054/149‑1 (MQ=255)
   ccTTCTTGGCCAGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTg                                                                                                                                                 <  3:73167/149‑1 (MQ=255)
    cttcttGGCCAGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTgg                                                                                                                                                >  2:75899/1‑149 (MQ=255)
          ggCCAGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCAAAGGCCTgg                                                                                                                                           <  1:511/148‑2 (MQ=255)
           gCCAGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAgc                                                                                                                                         >  3:346831/1‑149 (MQ=255)
            ccAGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAgcg                                                                                                                                        <  4:346831/149‑1 (MQ=255)
            ccAGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCAAAGGCCTggggc                                                                                                                                        <  3:124696/149‑5 (MQ=255)
              aGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCTCAGGCCAAAGGCCTggggct                                                                                                                                       <  1:136833/148‑6 (MQ=255)
              aGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCTCAGGCCAAAGGCCTggggct                                                                                                                                       >  2:136833/1‑143 (MQ=255)
                gCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAgcggcg                                                                                                                                     >  6:66995/1‑148 (MQ=255)
                 cTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGaa                                                                                                                                   <  5:61560/149‑1 (MQ=255)
                 cTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGaa                                                                                                                                   <  3:130450/149‑1 (MQ=255)
                 cTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGaa                                                                                                                                   <  3:102750/149‑1 (MQ=255)
                       gccgccGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCAtt                                                                                                                             <  5:66995/149‑1 (MQ=255)
                         cgccgcTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGt                                                                                                                           <  7:17180/149‑1 (MQ=255)
                           ccgcTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTAc                                                                                                                         <  6:132608/149‑1 (MQ=255)
                             gcTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTActg                                                                                                                       >  7:263640/1‑149 (MQ=255)
                             gcTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTActg                                                                                                                       <  7:20453/149‑1 (MQ=255)
                                 ccGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTgg                                                                                                                   <  2:353569/149‑1 (MQ=255)
                                   gggTTCTGGAGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCACGGTCACGTAGTTCTCGATATAGGCTTCATCCTTGACCAGCCCCAGGACATTGGCCTGAGCCGCGAAGGCAATGTCCTGCTCGGc                                                                                                                 >  8:163206/1‑149 (MQ=255)
                                         tGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGc                                                                                                            >  5:364105/1‑148 (MQ=255)
                                            cGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCTTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAAc                                                                                                        <  1:75899/149‑1 (MQ=255)
                                            cGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAAc                                                                                                        <  1:256263/149‑1 (MQ=255)
                                             gAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCAAGCGCCTTCTCGGTCACCTAGATCTCGATATTGGCGTCATCCTTGACAAGCCCCAGGCCTTTGGCCTGCGCGGCTAGGGCAATGTAATGCTGGGCCACGCCAAcc                                                                                                       >  7:116430/1‑149 (MQ=255)
                                             gAATGTTCTGCTCCTGTTTGCCCATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATAGGCGACATCCTTGACCAGCCCCAGGACTTTGGCCAGAGCGGCGAAGGCAATCTACTGCTGGGCCACGACAAc                                                                                                        >  4:14223/1‑148 (MQ=255)
                                                       cTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTgg                                                                                             <  8:263640/149‑1 (MQ=255)
                                                        tCCTGTTTGCCAATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGc                                                                                            >  4:154141/1‑149 (MQ=255)
                                                          cTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCTGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGGCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGcc                                                                                           <  7:163206/148‑1 (MQ=255)
                                                            gTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCACAGGCCTTTGGCCTGAGAGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTgg                                                                                        >  5:198488/1‑148 (MQ=255)
                                                             tttGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCGTTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCt                                                                                       >  2:237109/1‑149 (MQ=255)
                                                                 ccGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGa                                                                                    <  4:250326/148‑1 (MQ=255)
                                                                  cGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGc                                                                                        >  2:196323/1‑143 (MQ=255)
                                                                  cGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGc                                                                                        <  1:196323/143‑1 (MQ=255)
                                                                    atcatcTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGAt                                                                                >  3:70013/1‑149 (MQ=255)
                                                                    atcatcTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGAt                                                                                >  2:323984/1‑149 (MQ=255)
                                                                     tcatcTCGAACCGGCCATCGAGCGCCTTCTCGGTCACGTAGTTCTCGATTTTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGGCAACCTTGTCGGTGGCCAGCTTGACGAtt                                                                               <  4:52166/149‑1 (MQ=255)
                                                                        tctcGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTActgctg                                                                                                                    <  5:331138/109‑1 (MQ=255)
                                                                        tctcGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTActgctg                                                                                                                    >  6:331138/1‑109 (MQ=255)
                                                                        tctcGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTgg                                                                             >  5:33618/1‑148 (MQ=255)
                                                                          tcGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAg                                                                          >  3:263811/1‑149 (MQ=255)
                                                                            gAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAgg                                                                         >  6:317279/1‑148 (MQ=255)
                                                                            gAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAg                                                                          <  6:364105/147‑1 (MQ=255)
                                                                                aGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACtt                                                                    <  4:263811/149‑1 (MQ=255)
                                                                                aGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACtt                                                                    >  4:928/1‑149 (MQ=255)
                                                                                aGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACtt                                                                    >  4:162588/1‑149 (MQ=255)
                                                                                  gCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTCGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGc                                                                                        <  4:253765/127‑1 (MQ=255)
                                                                                  cccATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTCGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGc                                                                                        >  3:253765/2‑127 (MQ=255)
                                                                                   ccatccatCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGGCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGgc                                                                 <  8:32658/149‑1 (MQ=255)
                                                                                          gcgcCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGTGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATc                                                          <  7:266059/149‑1 (MQ=255)
                                                                                                 ctcGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTcgc                                                   >  5:284131/1‑149 (MQ=255)
                                                                                                    ggTCACGTAGTTCTCGATATTGTCGTCATCCTTGTCCAGCCCCAGGCCTTTTGCCTGAGCGTCGAGGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCgg                                                 <  8:116430/148‑1 (MQ=255)
                                                                                                    ggTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCGTTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCgg                                                 <  1:237109/148‑1 (MQ=255)
                                                                                                     gTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGgct                                               >  4:255705/1‑149 (MQ=255)
                                                                                                        aCGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGgctgct                                            <  1:227081/149‑1 (MQ=255)
                                                                                                          gTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGctct                                          >  3:343997/1‑149 (MQ=255)
                                                                                                            aGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTg                                        >  7:264632/1‑149 (MQ=255)
                                                                                                             gTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGt                                       >  1:46426/1‑149 (MQ=255)
                                                                                                                ctggATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTc                                     <  3:14223/145‑1 (MQ=255)
                                                                                                                ctcGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGgcgc                                                               >  6:106860/1‑122 (MQ=255)
                                                                                                                ctcGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGgcgc                                                               <  5:106860/122‑1 (MQ=255)
                                                                                                                ctcGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTc                                     <  3:162588/148‑1 (MQ=255)
                                                                                                                 tcGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAg                                   >  5:249532/1‑149 (MQ=255)
                                                                                                                   gttatTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGTAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCa                                    <  6:198488/144‑1 (MQ=255)
                                                                                                                   gATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCa                                    <  2:44970/146‑1 (MQ=255)
                                                                                                                   gATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCa                                    <  6:33618/146‑1 (MQ=255)
                                                                                                                         ggCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCGGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTgg                            <  1:167086/148‑1 (MQ=255)
                                                                                                                           cGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTGGGTg                         >  2:199984/1‑149 (MQ=255)
                                                                                                                             tCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGGACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTGGGTGGc                       >  1:178633/1‑149 (MQ=255)
                                                                                                                                     gACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTGGGTGGCCGAgtc                 <  6:284131/147‑1 (MQ=255)
                                                                                                                                       ccAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTGGGTGGCCGAGTCGTCa             >  7:306335/1‑149 (MQ=255)
                                                                                                                                             ccAGGCCTTTGGCCTGCGCGGCGAAGGCATTGTACTGCTGGGCCACACCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGAGGCTGCTCTTGTTCAGGTACTGGGTGGCCGAGTCGTCCccgcc        >  2:159919/1‑148 (MQ=255)
                                                                                                                                             ccAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTGGGTGGCCGAGTCGTCAccgccg       >  8:232248/1‑149 (MQ=255)
                                                                                                                                             ccAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTGGGTGGCCGAGTCGTCAccgccg       >  5:236540/1‑149 (MQ=255)
                                                                                                                                                 gCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTGGGTGGCCGAGTCGTCACCGCCGCTga   <  1:323984/149‑1 (MQ=255)
                                                                                                                                                   cTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGAGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTGGGTCGCCGAGTCGTCACCGCCGCTgag  >  8:22473/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGACCTTCTTGGCCAGGCTGGTAGCCGCCGCTGCCGGGTTCTGGCGAATGTTCTGCTCCTGTTTGCCGATCATCTCGAACAGGCCATCCAGCGCCTTCTCGGTCACGTAGTTCTCGATATTGGCGTCATCCTTGACCAGCCCCAGGCCTTTGGCCTGAGCGGCGAAGGCATTGTACTGCTGGGCCACGCCAACCTTGTCGGTGGCCTGCTTGACGATTGGCAGGAACTTGGCGCGGATCTGCTCGCGGCTGCTCTTGTTCAGGTACTGGGTGGCCGAGTCGTCACCGCCGCTGAG  >  NC_002947/1724496‑1724790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑