Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,731,767 C→T 7.4% S701S (TCG→TCA)  PP_1526 ← beta‑(1‑3)‑glucosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,731,7670CT 7.4% 158.8 / 2.3 54S701S (TCG→TCA) PP_1526beta‑(1‑3)‑glucosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (27/23);  new base T (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

CGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCTTTGCCGCGCAGCAGCGCGCCGGCGTGGTGCTTGATGATC  >  NC_002947/1731622‑1731914
                                                                                                                                                 |                                                                                                                                                    
cGAAGGCATCCTTGCGGTTCACCCCCCCCGCTCGGCGGTACAGGAAGATCATCTTGCCGACCTTCAAGAAGAACCGCGCCCGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGCCCCGTTGCGGCACGATGATCATCGCCGCCGAc                                                                                                                                                   <  1:17118/149‑1 (MQ=255)
cGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGAc                                                                                                                                                   <  2:59101/149‑1 (MQ=255)
         ccTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAgc                                                                                                                                          >  3:358726/1‑149 (MQ=255)
          cTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAgcg                                                                                                                                         >  8:21694/1‑149 (MQ=255)
           ttGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAgcgc                                                                                                                                        >  3:15271/1‑149 (MQ=255)
                     cccccACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTg                                                                                                                              <  8:98027/149‑1 (MQ=255)
                      ccccACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCacaaca                                                                                                                                            <  1:305885/134‑1 (MQ=255)
                      ccccACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCacaaca                                                                                                                                            >  2:305885/1‑134 (MQ=255)
                      ccccACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTg                                                                                                                              >  2:290033/1‑148 (MQ=255)
                      ccccACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGAGAAGATCATCAGGGGCTGATCGACCCGATGCGGCACGATCATCATCGCCGCCGACCACAACAGCGCGCCGATGCTg                                                                                                                              >  3:238873/1‑148 (MQ=255)
                           ccGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGaag‑aag                                                                                                                       <  7:21694/149‑1 (MQ=255)
                             gCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGAt                                                                                                                     <  5:11015/149‑1 (MQ=255)
                               tCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGt                                                                                                                   >  1:16365/1‑149 (MQ=255)
                                 ggcggTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTc                                                                                                                 <  4:358726/149‑1 (MQ=255)
                                          ggAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCg                                                                                                        >  5:44680/1‑149 (MQ=255)
                                           gAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAGG‑AAGATGTTCATGCCATCgg                                                                                                       >  4:77619/1‑149 (MQ=255)
                                           gAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCgg                                                                                                       <  1:305942/149‑1 (MQ=255)
                                                        ccGACCTTGACGCAGACCAGCTCCAGCGGCGGGATGGCGAAGATCATCAGGCGCGGATCGAGCAGATGCGGCACGTTGATCATCTCGGCCTACCACATCAGCGCGCCGATGGTGAAG‑AAGATGTTCATGACATCGGCGTTCCACGGTa                                                                                           <  4:238873/148‑1 (MQ=255)
                                                        ccGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTc                                                                                                                 >  2:191041/1‑126 (MQ=255)
                                                        ccGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTc                                                                                                                 <  1:191041/126‑1 (MQ=255)
                                                             cTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTagccagc                                                                                     >  1:234603/1‑149 (MQ=255)
                                                                gaagaagaaCAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCgg                                                                                  >  6:22783/1‑149 (MQ=255)
                                                                  agaagaaCAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGTCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTagccagcgctac                                                                                >  8:115392/1‑144 (MQ=255)
                                                                  agaagaaCAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGc                                                                                 <  5:22783/148‑1 (MQ=255)
                                                                   gaagaaCAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGc                                                                                 >  1:104139/1‑147 (MQ=255)
                                                                    aagaaCAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAg                                                                              <  4:9485/149‑1 (MQ=255)
                                                                          aGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTg                                                                        <  4:15271/149‑1 (MQ=255)
                                                                           gcgcCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTgg                                                                       <  4:238504/149‑1 (MQ=255)
                                                                            cgcCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTgg                                                                       >  6:302635/1‑148 (MQ=255)
                                                                             gcCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTgg                                                                       >  1:42775/1‑147 (MQ=255)
                                                                             gcCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTa                                                                     <  1:162288/149‑1 (MQ=255)
                                                                                    gcggGATGGCGAAGATCATCATGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAGTAAGATGTTCATGCCATCGGCGATCCACGGTAGCCGGCCGGCCAGGAAGTGGTAGCGCTg                                                               <  1:290033/149‑1 (MQ=255)
                                                                                      gggATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGc                                                             >  1:79305/1‑148 (MQ=255)
                                                                                              gAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCAc                                                          <  5:86934/143‑1 (MQ=255)
                                                                                              gAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCAc                                                          >  6:86934/1‑143 (MQ=255)
                                                                                                aGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAgc                                                  <  2:42775/149‑1 (MQ=255)
                                                                                                    catcaGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCAAAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAgctggc                                              >  6:169835/1‑149 (MQ=255)
                                                                                                      tcaGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATcc                                                                                                 <  8:147064/96‑1 (MQ=255)
                                                                                                      tcaGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATcc                                                                                                 >  7:147064/1‑96 (MQ=255)
                                                                                                         ggggCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAg                                                                    >  8:288431/1‑122 (MQ=255)
                                                                                                         ggggCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAg                                                                    <  7:288431/122‑1 (MQ=255)
                                                                                                         ggggCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCt                                         >  3:268451/1‑149 (MQ=255)
                                                                                                            gCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCTTTg                                      >  1:231426/1‑149 (MQ=255)
                                                                                                            gCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCTTTg                                      >  5:357480/1‑149 (MQ=255)
                                                                                                              ggATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCTTTGcc                                    >  6:61678/1‑149 (MQ=255)
                                                                                                                   gACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCTTTGCCgcgc                                <  2:234603/148‑1 (MQ=255)
                                                                                                                      ccGATGCGGCACGATGATCATCGCCGCTGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGc                                                                                 >  4:202278/1‑96 (MQ=255)
                                                                                                                      ccGATGCGGCACGATGATCATCGCCGCTGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGc                                                                                 <  3:197540/96‑1 (MQ=255)
                                                                                                                      ccGATGCGGCACGATGATCATCGCCGCTGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGc                                                                                 <  3:202278/96‑1 (MQ=255)
                                                                                                                      ccGATGCGGCACGATGATCATCGCCGCTGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGc                                                                                 >  4:197540/1‑96 (MQ=255)
                                                                                                                                  gatgatCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCTTTGCCGCGCAGCAGCGCGCCGGCgt                >  7:192651/1‑149 (MQ=255)
                                                                                                                                    tgatCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCTTTGCCGCGCAGCAGCGCGCCGGCgtgg              <  2:16365/149‑1 (MQ=255)
                                                                                                                                         atcGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACgg                                                        >  7:325464/1‑102 (MQ=255)
                                                                                                                                         atcGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACgg                                                        <  8:325464/102‑1 (MQ=255)
                                                                                                                                            gccgccGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCTTTGCCGCGCAGCAGCGCGCCGGCGTGGTGCTtga       <  2:104139/148‑1 (MQ=255)
                                                                                                                                                ccgACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCTTTGCCGCGCAGCAGCGCGCCGGCGTGGTGCTTGATGATc  >  5:146623/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACCTTGAAGAAGAACAGCGCCAGCGGCGGGATGGCGAAGATCATCAGGGGCGGATCGACCCGATGCGGCACGATGATCATCGCCGCCGACCACAACAGCGCGCCGATGGTGAAG‑AAGATGTTCATGCCATCGGCGATCCACGGTAGCCAGCCGGCCAGGAAGTGGTAGCGCTGGCCACGGGTCAGCTGGCTGCCTTTGCCGCGCAGCAGCGCGCCGGCGTGGTGCTTGATGATC  >  NC_002947/1731622‑1731914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑