Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,809,191	G→T	9.9%	G81V (GGC→GTC)	eno →	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,809,191	0	G	T	9.9%	167.6 / 6.6	58	G81V (GGC→GTC)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base G (24/27);  new base T (3/3);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTG  >  NC_002947/1809043‑1809339                                                                                                                                                     |                                                                                                                                                     atcGGCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGACTCGCGCGAAGCGGTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCATCATCAACGGCCCGATCCGTGACCTGCTGCTggg                                                                                                                                                      <  1:169212/149‑1 (MQ=255)    ggCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCaa                                                                                                                                                   <  5:215357/149‑1 (MQ=255)    ggCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCaa                                                                                                                                                   <  5:223024/149‑1 (MQ=255)    ggCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCaa                                                                                                                                                   <  5:186350/149‑1 (MQ=255)    ggCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCaa                                                                                                                                                   <  4:170062/149‑1 (MQ=255)    ggCAGCGCTTGCGCGCCGTCCGGTGCTTCTACAGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGCCAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGc                                                                                                                                                     >  5:285281/1‑147 (MQ=255)     gCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTCGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGCAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAg                                                                                                                                                  >  1:272574/1‑149 (MQ=255)      cAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAGGTCacg                                                                                                                                                  >  4:151667/1‑146 (MQ=255)          gcTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAGGTCACGGATcg                                                                                                                                             <  3:151667/149‑2 (MQ=255)           cTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAGGTCACGGATcgg                                                                                                                                            >  2:269606/1‑147 (MQ=255)            ttGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCtt                                                                                                                                           >  6:77220/1‑149 (MQ=255)              gcgcgcCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTc                                                                                                                                          <  7:189553/148‑1 (MQ=255)              gcgcgcCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGACAAGGATCCTTc                                                                                                                                          <  2:312817/148‑1 (MQ=255)               cgcgcCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAGGTCACGGATcgggcc                                                                                                                                         <  1:269606/148‑6 (MQ=255)               cgcgcCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTcc                                                                                                                                         <  5:218870/148‑1 (MQ=255)                 cgcCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGAt                                                                                                                                      <  5:77220/149‑1 (MQ=255)                  gcCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTc                                                                                                                                          <  7:39391/144‑1 (MQ=255)                  gcCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTc                                                                                                                                          >  8:39391/1‑144 (MQ=255)                  gcCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATc                                                                                                                                     <  2:286624/149‑1 (MQ=255)                        cGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAgg                                                                                                                               >  1:326410/1‑149 (MQ=255)                          gTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTTCGATCAGAAGGct                                                                                                                             >  2:139477/1‑149 (MQ=255)                          gTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGct                                                                                                                             >  7:6363/1‑149 (MQ=255)                              ttCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTg                                                                                                                          >  4:264126/1‑148 (MQ=255)                                cTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGa                                                                                                                        <  8:322205/148‑1 (MQ=255)                                   ccGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACcgc                                                                                                                    >  3:206323/1‑149 (MQ=255)                                      gcgcgcgcgAAGCGCGGGAGCGGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAAAATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGcc                                                                                                                 <  6:285281/146‑1 (MQ=255)                                          gcgcgAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGa                                                                                                             <  3:264126/149‑1 (MQ=255)                                                aGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAAc                                                                                                       >  6:205996/1‑149 (MQ=255)                                                  cgcTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTg                                                                                                     <  5:270813/149‑1 (MQ=255)                                                   gcTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTgg                                                                                                    <  2:326410/149‑1 (MQ=255)                                                     tGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGAc                                                                                                  >  1:339626/1‑149 (MQ=255)                                                         gCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTa                                                                                              >  7:87550/1‑149 (MQ=255)                                                               tGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAGGTCACGGATcgg                                                                                                                                            >  8:335040/1‑95 (MQ=25)                                                               tGATGGCGACAAGAGCCGTTACCTGGGAAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAGGTCACGGATcgg                                                                                                                                            <  7:335040/97‑3 (MQ=25)                                                                      gACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGc                                                                                 >  7:221317/1‑149 (MQ=255)                                                                                gTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGgc                                                                       <  8:221317/149‑1 (MQ=255)                                                                                    ccTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGcc                                                                    >  4:173655/1‑148 (MQ=255)                                                                                        ggCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGc                                                               >  7:76523/1‑149 (MQ=255)                                                                                         gCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGcc                                                              <  8:6363/149‑1 (MQ=255)                                                                                             gggCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATcc                                                          >  5:41588/1‑149 (MQ=255)                                                                                                cGTGCTGAAGGTCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTgg                                                       <  1:128275/149‑1 (MQ=255)                                                                                                 gTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGc                                                      <  5:205996/149‑1 (MQ=255)                                                                                                       aaGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCAATCCTGGCTGTTTc                                                >  8:52794/1‑149 (MQ=255)                                                                                                                accAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGc                                                               >  4:103595/2‑125 (MQ=255)                                                                                                                accAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGc                                                               <  3:103595/124‑1 (MQ=255)                                                                                                                           cGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAgctg                            <  5:171535/149‑1 (MQ=255)                                                                                                                            ggCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACAGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAgctgc                           >  4:241032/1‑149 (MQ=255)                                                                                                                             gCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTTCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAgctgct                          <  1:139477/149‑1 (MQ=255)                                                                                                                                 gATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGccc                      <  6:41588/149‑1 (MQ=255)                                                                                                                                 gATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGcc                       >  6:346227/1‑148 (MQ=255)                                                                                                                                       ggACCTGCTGCTGGGCACGGATCCTTCCGCTCAGCAGGCTCTGGCCCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCccaggacc                <  2:272574/148‑1 (MQ=255)                                                                                                                                        gACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCccaggacca               <  4:206323/149‑1 (MQ=255)                                                                                                                                         aCCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCATGGCTGCCGCCAAAGCTGCTGCccaggaccag              >  7:278189/1‑149 (MQ=255)                                                                                                                                           ctgctgctgGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATcc                                                          >  7:296143/1‑103 (MQ=255)                                                                                                                                           ctgctgctgGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATcc                                                          <  8:296143/103‑1 (MQ=255)                                                                                                                                           ctgctgctgGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCccaggaccagga            <  8:87550/149‑1 (MQ=255)                                                                                                                                            tgctgctgGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCccagg                   >  2:159503/1‑141 (MQ=255)                                                                                                                                            tgctgctgGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCccagg                   <  1:159503/141‑1 (MQ=255)                                                                                                                                            tgctgctgGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCcaggaccaggac           >  6:309572/1‑149 (MQ=255)                                                                                                                                                    ggCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCt   <  3:173655/149‑1 (MQ=255)                                                                                                                                                    ggCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCt   >  3:110274/1‑149 (MQ=255)                                                                                                                                                    ggCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCt   <  2:339626/149‑1 (MQ=255)                                                                                                                                                     gCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTg  >  4:176290/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     ATCGGCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTG  >  NC_002947/1809043‑1809339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑