Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,853,197 A→T 9.5% intergenic (+42/‑82) relA → / → mazG ATP:GTP 3'‑pyrophosphotransferase/modified nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,853,1970AT 9.5% 151.8 / 4.8 52intergenic (+42/‑82)relA/mazGATP:GTP 3'‑pyrophosphotransferase/modified nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (24/23);  new base T (2/3);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGCTGACCATCGAGATTCCAGGCCTGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGC  >  NC_002947/1853050‑1853344
                                                                                                                                                   |                                                                                                                                                   
gTCGCTGACCATCGAGATTCCAGGCCTGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAggat                                                                                                                                                    >  8:198699/1‑149 (MQ=255)
         cATCGAGATTCCAGGCCTGGACGCGCTGGGGCGCCTGCTGGGGAGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCCGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAgg                                                                                                                                           >  1:257398/1‑149 (MQ=255)
           tCGAGATTCCAGGCCTGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGtt                                                                                                                                         >  2:216769/1‑149 (MQ=255)
                   ccAGGCCTGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATc                                                                                                                                  <  7:344752/148‑1 (MQ=255)
                    cAGGCCTGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCt                                                                                                                                 <  3:6095/148‑1 (MQ=255)
                    cAGGCCTGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTg                                                                                                                                <  6:169683/149‑1 (MQ=255)
                    cAGGCCTGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTg                                                                                                                                >  4:344713/1‑149 (MQ=255)
                     aGGCCTGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGTTGGATGCAGGTTTGAAATCTg                                                                                                                                >  4:68974/1‑148 (MQ=255)
                     aGGCCTGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGCTTGCCCGCTCCCACAGGTACGGTGCAGATTTCAAACCTg                                                                                                                                >  3:194501/1‑148 (MQ=25)
                           ggACGCGCTGGGGCGCCTGCTGGGGCTGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATAGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCagagta                                                                                                                         >  6:11489/1‑144 (MQ=255)
                                cgcTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCtgt                                                                                                                    <  7:77868/149‑1 (MQ=255)
                                 gcTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGCTTTGAAATCTGCACCGTACCtgtg                                                                                                                   >  7:327593/1‑149 (MQ=255)
                                  cTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTgg                                                                                                                  <  4:23066/149‑1 (MQ=255)
                                      ggCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCCACAGGTACGGTGCAGATTTCAAACCTGCATCCATCCTGTAGGAGc                                                                                                              <  3:108567/149‑1 (MQ=21)
                                      ggCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCCACAGGTACGGTGCAGATTTCAAACCTGCATCCATCCTGTAGGAGc                                                                                                              <  7:139201/149‑1 (MQ=21)
                                       gcgcCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGc                                                                                                              >  1:90853/1‑148 (MQ=255)
                                       gcgcCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGc                                                                                                              <  2:90853/148‑1 (MQ=255)
                                       gcgcCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCg                                                                                                             >  8:341730/1‑149 (MQ=255)
                                       gcgcCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGCTTGCCCGCTCCCACAGGTACGGTGCAGATTTCAAACCTGCATCCATCCTGTAGGAGCg                                                                                                             <  4:194501/149‑1 (MQ=17)
                                           ctgcagGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGc                                                                                                          >  3:40283/1‑148 (MQ=255)
                                                     ggATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCgaa                                                                                               <  3:45938/149‑1 (MQ=255)
                                                           cGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGc                                                                                          >  3:131625/1‑148 (MQ=255)
                                                           cGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGc                                                                                          >  5:51629/1‑148 (MQ=255)
                                                            gCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCgaagaa                                                                                            <  7:33622/145‑1 (MQ=255)
                                                                          tcatcGAGACGCGGCGTAATCATACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcct                                                                          >  6:225548/1‑149 (MQ=255)
                                                                            atcGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcctgc                                                                        <  4:40283/149‑1 (MQ=255)
                                                                             tcGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcctgcc                                                                       >  4:326645/1‑149 (MQ=255)
                                                                                   cgcgGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATg                                                                 <  3:344713/149‑1 (MQ=255)
                                                                                    gcggcgTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGt                                                                    <  4:300221/145‑1 (MQ=255)
                                                                                    gcggcgTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGt                                                                    >  3:300221/1‑145 (MQ=255)
                                                                                        cgTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTa                                                            >  8:351580/1‑149 (MQ=255)
                                                                                        cgTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTa                                                            <  7:341730/149‑1 (MQ=255)
                                                                                          tAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTaca                                                          <  4:131625/149‑1 (MQ=255)
                                                                                            aTCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCgaa                                                                                               >  7:72117/1‑110 (MQ=255)
                                                                                            aTCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCgaa                                                                                               <  8:72117/110‑1 (MQ=255)
                                                                                            aTCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcct                                                                          <  5:229029/131‑1 (MQ=255)
                                                                                            aTCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcct                                                                          >  6:229029/1‑131 (MQ=255)
                                                                                                  ccccTTGAGACCCGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTgac                                                  <  2:257398/149‑1 (MQ=255)
                                                                                                    ccTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTgacga                                                <  3:326645/149‑1 (MQ=255)
                                                                                                     cTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGAcc                                                              <  7:186326/134‑1 (MQ=255)
                                                                                                     cTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGAcc                                                              >  8:186326/1‑134 (MQ=255)
                                                                                                           aCCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGAGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACctgct                                          >  8:85980/1‑148 (MQ=255)
                                                                                                                   gTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTac                                                           <  2:312860/123‑1 (MQ=255)
                                                                                                                   gTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTac                                                           >  1:312860/1‑123 (MQ=255)
                                                                                                                    tCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATg                                <  1:216769/149‑1 (MQ=255)
                                                                                                                      tGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGc                              >  1:306088/1‑149 (MQ=255)
                                                                                                                        cTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGccc                            <  5:225548/149‑1 (MQ=255)
                                                                                                                         ttCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGGTTGAAATCTGCACCGTACCTGTGGGAGCGGGTAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCg                           >  5:35078/1‑149 (MQ=255)
                                                                                                                                gTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTgcgc                    >  5:54628/1‑149 (MQ=255)
                                                                                                                                       cGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCg              >  6:331901/1‑148 (MQ=255)
                                                                                                                                             tACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACg       >  6:249988/1‑149 (MQ=255)
                                                                                                                                               cAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGc     <  6:54628/149‑1 (MQ=255)
                                                                                                                                                aGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCt    <  5:249988/149‑1 (MQ=255)
                                                                                                                                                  gatggatgCAGGTTTGAAATCTGCACCTTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGc  <  3:68974/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCGCTGACCATCGAGATTCCAGGCCTGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGC  >  NC_002947/1853050‑1853344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑