Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,859,108	C→G	6.1%	intergenic (+28/+73)	PP_1661 → / ← PP_1662	dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,859,108	0	C	G	6.1%	205.3 / 3.1	65	intergenic (+28/+73)	PP_1661/PP_1662	dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/29);  new base G (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CGTGGCCAGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAA  >  NC_002947/1858961‑1859255                                                                                                                                                     |                                                                                                                                                    cgTGGCCAGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGGCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTcc                                                                                                                                                    <  3:34012/149‑1 (MQ=255)       cAGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCgg                                                                                                                                                  <  1:296685/145‑1 (MQ=255)       cAGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCgg                                                                                                                                                  >  2:296685/1‑145 (MQ=255)       cAGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAAt                                                                                                                                              >  8:190658/1‑149 (MQ=255)            aGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTAAGG‑GGCTGGCCTGAGAGATAACCTGGGCAGCCTGACGGTAGAGGTGAGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCACCGCCAATGACg                                                                                                                                          >  2:332229/1‑148 (MQ=255)                gAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCTgcggca                                                                                                                                     >  2:176004/1‑149 (MQ=255)                     gCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTg                                                                                                                                >  2:354470/1‑149 (MQ=255)                       aGCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCTGCGGCAttgccgg                                                                                                                              <  1:176004/149‑6 (MQ=255)                       aGCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTggg                                                                                                                              <  7:171167/149‑1 (MQ=255)                        gCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTgggg                                                                                                                             <  1:354470/149‑1 (MQ=255)                            tgCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGtt                                                                                                                           <  7:190658/147‑1 (MQ=255)                              cATCAATGACGAACTGGACGGGCGTTACGGAGCCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGTTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGc                                                                                                                         >  1:33314/1‑148 (MQ=255)                               aTCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCa                                                                                                                      >  2:134633/1‑149 (MQ=255)                                 cAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACt                                                                                                                    >  8:46320/1‑149 (MQ=255)                                             ggACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGc                                                                                                          >  4:261148/1‑147 (MQ=255)                                              gACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCTGTAGAGGTGCGTTTCTGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGa                                                                                                        <  2:33314/148‑1 (MQ=255)                                                  ggCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAg                                                                                                   <  3:261148/149‑1 (MQ=255)                                                    cGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAggg                                                                                                 <  4:326568/149‑1 (MQ=255)                                                    cGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAggg                                                                                                 <  8:185904/149‑1 (MQ=255)                                                    cGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAggg                                                                                                 <  3:177453/149‑1 (MQ=255)                                                     gTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTg                                                                                                                                <  8:129747/117‑1 (MQ=255)                                                     gTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTg                                                                                                                                >  7:129747/1‑117 (MQ=255)                                                     gTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAgggg                                                                                                >  2:318290/1‑149 (MQ=255)                                                         cGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTc                                                                                             <  7:304376/148‑1 (MQ=255)                                                          gg‑ggCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTcgc                                                                                           <  1:332229/149‑1 (MQ=255)                                                             ggCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTcgccg                                                                                         >  5:265128/1‑149 (MQ=255)                                                                  gCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCaa                                                                                      <  1:134633/147‑1 (MQ=255)                                                                  gCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATg                                                                                    >  1:62492/1‑149 (MQ=255)                                                                  gCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATg                                                                                    >  4:32393/1‑149 (MQ=255)                                                                       aGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAg                                                                               <  8:55098/149‑1 (MQ=255)                                                                        gCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAgg                                                                              <  4:348346/149‑1 (MQ=255)                                                                          gATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATg                                                                                    <  5:268725/141‑1 (MQ=255)                                                                          gATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCAGCAATg                                                                                    >  6:268725/1‑141 (MQ=255)                                                                           aTAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGTTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGt                                                                            >  3:349488/1‑148 (MQ=255)                                                                              aCCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCAtt                                                                        <  3:32393/149‑1 (MQ=255)                                                                              aCCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCAt                                                                         <  7:46320/148‑1 (MQ=255)                                                                                cTGGGCAGCCTGTCGGTAGAGGTGCGGTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtgt                                                                      <  4:155562/149‑1 (MQ=255)                                                                                  gggCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtgtgt                                                                    >  7:39981/1‑149 (MQ=255)                                                                                      aGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGgcg                                                                >  7:204752/1‑149 (MQ=255)                                                                                               gTAGAGGTGCGTTTCGGGGCGGTGTAGGTCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGttt                                                       >  8:286765/1‑149 (MQ=255)                                                                                                     gTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAgg                                                 >  3:259362/1‑149 (MQ=255)                                                                                                      tGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAg                                                  <  3:59540/147‑1 (MQ=255)                                                                                                      tGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAg                                                  >  4:59540/1‑147 (MQ=255)                                                                                                         gTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCt                                              >  2:277363/1‑148 (MQ=255)                                                                                                              ggggCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGc                                        >  4:289974/1‑149 (MQ=255)                                                                                                               gggCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCt                                       <  6:308243/149‑1 (MQ=255)                                                                                                                         ggCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTa                             <  8:39981/149‑1 (MQ=255)                                                                                                                           cccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTAtt                           >  1:159683/1‑149 (MQ=255)                                                                                                                            ccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTg                          >  3:171470/1‑149 (MQ=255)                                                                                                                            ccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGGCTTCAAGCTCAAGGCGGTATTg                          >  3:55650/1‑149 (MQ=255)                                                                                                                                 cATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGtgtg                     >  2:103446/1‑149 (MQ=255)                                                                                                                                        aCCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCgtg              >  8:232521/1‑149 (MQ=255)                                                                                                                                         ccccACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGgt                        >  3:247303/1‑138 (MQ=255)                                                                                                                                         ccccACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGgt                        <  4:247303/138‑1 (MQ=255)                                                                                                                                          cccaaaaTGCTGCGGCATTGCCGGAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCgtgt             >  2:174147/7‑148 (MQ=38)                                                                                                                                          cccaaaaTGCTGCGGCATTGCCGGAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCgtgt             <  1:174147/142‑1 (MQ=38)                                                                                                                                          cccaaaaTGCTGCGGCATTGCCGGAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCgtg              <  6:57902/141‑1 (MQ=38)                                                                                                                                          cccaaaaTGCTGCGGCATTGCCGGAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCgtg              >  5:57902/7‑147 (MQ=38)                                                                                                                                           ccACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTc           >  2:31265/1‑149 (MQ=255)                                                                                                                                            cACGATTCTCCGGCAATGCCGGAGCATTTTGGGGTTGCTCTCTGAGCCGAGGCGAGTCAGGGGTTCGCCGGAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCg          <  8:204752/149‑1 (MQ=255)                                                                                                                                            cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCg          >  6:177296/1‑149 (MQ=255)                                                                                                                                                aTGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTAt      <  2:62492/149‑1 (MQ=255)                                                                                                                                                 tGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAAGGCGGTATTGGTGTGCTTCGTGTTCGTTATg     <  1:31265/149‑1 (MQ=255)                                                                                                                                                 tGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTAt      <  4:259362/148‑1 (MQ=255)                                                                                                                                                 tGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATg     <  3:289974/149‑1 (MQ=255)                                                                                                                                                    tCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGAGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGAGCGCTGGTTTTCCAGGTATTCAAGCTCAAAGCGGTATTGGTGTGCTTCGTGTTCGTTATGGaa  >  7:62193/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CGTGGCCAGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGG‑GGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAA  >  NC_002947/1858961‑1859255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑