| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 197,185 | C→T | 5.6% | T897T (ACC→ACT) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 197,185 | 0 | C | T | 5.6% | 531.3 / 14.2 | 180 | T897T (ACC→ACT) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (83/85); new base T (5/5); total (89/91) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
Only 100 of 184 total aligned reads displayed.
GCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCG > NC_002947/197037‑197329 | tCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTa > 1:296676/1‑148 (MQ=37) aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTaca‑c > 7:266959/1‑149 (MQ=37) ccATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACA‑cc < 3:282236/149‑1 (MQ=17) ccATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑cc > 1:13383/1‑149 (MQ=37) ccATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑cc > 5:128736/1‑149 (MQ=37) cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACA‑CCg > 2:69018/1‑149 (MQ=17) cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCg > 2:206792/1‑149 (MQ=37) cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCg > 1:144963/1‑149 (MQ=37) aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGTCGTAATC‑ACCTACA‑CCGt < 3:151303/149‑1 (MQ=37) tCGACAACGCCACAGGCGGTAATTTCGAGCAACTGACACCGAACCAGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGCTCGATGACACCACCGCGACCCTGACGGCCAGCCCGTCGGTCACCGAATCCGCCGTAAAC‑ACCTACA‑CCGt > 7:43233/1‑148 (MQ=11) cGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGa < 6:202222/149‑1 (MQ=37) gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCTGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCCCCGAAGGCGGCGTGATA‑ACTTACA‑CCGTGAc > 7:151491/1‑149 (MQ=11) gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGa < 5:187514/148‑1 (MQ=37) agaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGCCCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGGcc > 8:156984/1‑149 (MQ=255) agaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGAcc > 5:156461/1‑149 (MQ=37) agaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGAcc > 4:178796/1‑149 (MQ=37) cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGa < 7:301298/149‑1 (MQ=37) cGCCACAGGCGGGAACTTCGAGCCACTGCCCCCGACCCCGACCCCGGCTCAG‑CC‑CACGATCAACGACTCGGGCGATGCCACCACCGCGCCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGa < 4:297267/149‑1 (MQ=11) gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGTCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAg < 2:268081/149‑1 (MQ=37) gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑Acc < 2:150614/132‑1 (MQ=37) gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAg < 8:272651/149‑1 (MQ=37) ccACAGGCTTTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGCTCACACGATCAAGCTCTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGa < 1:119796/149‑1 (MQ=11) ccACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACA‑CCGTGACCCTGa > 6:314153/1‑147 (MQ=17) cacaGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGa < 2:169969/146‑1 (MQ=37) cacaGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGa > 1:169969/1‑146 (MQ=37) aGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATc < 3:75707/149‑1 (MQ=35) aGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATc < 2:296676/149‑1 (MQ=25) ggCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAACCTACA‑CCGTGACCCTGAGCAATc < 7:230737/149‑1 (MQ=21) ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGc > 2:331036/1‑149 (MQ=34) gTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGCAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGcc > 2:351977/1‑149 (MQ=21) tAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAT‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑AACTACA‑CCGTGACCCTGAGCAATCCTGcc < 3:201058/148‑1 (MQ=21) tAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGc < 3:362364/147‑1 (MQ=34) aaTTTCGAGCAACTGACCCCGAATCCGCCCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGcc > 5:178235/1‑147 (MQ=21) gagctccTGCCCACGAATCCTCCCCCGGCTCAG‑AC‑CAGGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGc < 8:101181/142‑1 (MQ=11) gAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACACCCGTGACCCTGAGCAATCCTGCCCAGACGc < 1:22328/149‑1 (MQ=21) gAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGa > 3:56121/1‑130 (MQ=35) gCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCgg < 1:98397/149‑1 (MQ=34) cAACTGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGt < 3:222225/149‑1 (MQ=21) aCTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTaa > 3:132303/1‑149 (MQ=35) aCTGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGtga > 8:226616/1‑147 (MQ=14) tGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGAACCTGAGCAATCCTGCCCAGACGCCGGTAAcc > 5:372365/1‑149 (MQ=37) tGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑cc > 8:110286/1‑113 (MQ=17) tGACCCCGAACCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGtga > 7:109728/1‑145 (MQ=11) aCCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCg < 6:156461/148‑1 (MQ=38) ccccGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGa < 4:185748/120‑1 (MQ=35) cccGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGAccc < 6:309175/116‑1 (MQ=35) cccGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGAccc > 5:309175/1‑116 (MQ=35) ccGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAg > 7:24900/1‑119 (MQ=35) ccGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAg < 8:24900/119‑1 (MQ=35) ccGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAc > 1:15613/1‑149 (MQ=39) ccGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAc > 2:221476/1‑149 (MQ=39) cGAATCCGACCCCGGCTCAG‑AC‑CACGATATAGGACTCGGTCGATGCCACTACCGCGACCCTGACGGCGAGTCAGTCTTTCACTGAAGGAGGAGTGATC‑TCTTACA‑CCTTGACGCTGAGCAAGCATTCCCAGACGCCGGTTACCGTGAcc < 6:368793/149‑1 (MQ=2) cccGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTaca < 2:61589/95‑1 (MQ=34) cccGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACg > 1:239737/1‑149 (MQ=35) cccGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACg > 6:179650/1‑149 (MQ=35) ccGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATc > 7:19320/1‑112 (MQ=32) ccGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATc < 8:19320/112‑1 (MQ=32) cGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGTGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGc > 2:163469/1‑149 (MQ=21) cGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGc < 2:23335/149‑1 (MQ=34) aC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGtcat < 4:10388/149‑1 (MQ=39) tCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGtcatca < 1:217839/144‑1 (MQ=40) tCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGtt < 1:72330/149‑1 (MQ=255) aaCGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGGCCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTg < 7:156984/148‑1 (MQ=255) aaCGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGt < 4:250774/124‑1 (MQ=35) aCGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGaa < 1:331036/149‑1 (MQ=255) cGACTCGGTCCATGCCACCACCGCGACCCTGACGGCGAGACAGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGACATCACTGTTGacg > 1:367006/1‑147 (MQ=255) gACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGc > 4:262654/1‑149 (MQ=255) ggTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAATGGCCAGGTCATCACTGTTGAAGCCgg < 1:196298/147‑1 (MQ=255) gTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCa > 5:70841/1‑148 (MQ=255) tCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGccc > 7:309351/1‑90 (MQ=33) gATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAAc > 3:100633/1‑149 (MQ=255) ccaccaCCGCGACCCTGACGGCGAGCCGGTCGGACACCGAAGCCGGCGTAATC‑ACCTACA‑CCGGGACCCTGAGCAATCCTCCCCCGACGCCGGTAACCGTGACCCTGTCGCACGGCCAGCTCATCACTGATGAATCCTGCAAGACCCAg > 7:256540/1‑149 (MQ=14) ccaccaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCa < 7:165793/148‑1 (MQ=255) caccacCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTc < 5:235586/108‑1 (MQ=35) caccacCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCg < 5:327981/137‑1 (MQ=255) caccacCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAgg > 4:63294/1‑149 (MQ=255) accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAg > 3:338387/1‑117 (MQ=37) accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAg < 4:338387/117‑1 (MQ=37) gcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCTAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGt > 3:180926/1‑149 (MQ=255) cgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACCTACA‑CCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTc < 6:130625/149‑1 (MQ=255) ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAtt > 3:292122/1‑149 (MQ=255) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa < 6:292661/149‑1 (MQ=255) cccGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTg < 4:358884/104‑1 (MQ=255) ccGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGc < 5:346023/149‑1 (MQ=255) gTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGa < 2:217705/44‑1 (MQ=38) tCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCaa < 4:132303/149‑1 (MQ=255) gggcACCGAAGGCGGCTTAATC‑ACCTACA‑CCTTGACCCTGAGCAATCCTGCCCAGTCGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAAt < 8:336252/145‑1 (MQ=255) tcacgtaaGGCGGCTTAATC‑ACGACCA‑CCTGGACCCTGATCTATACTCCCCAGACGCCGGTAACCGTGACCCTGTCCAACTGACAGGTCATCACTGTTGAAGCCTGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGAc < 2:367006/143‑1 (MQ=14) tCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGAc < 1:340604/149‑1 (MQ=40) ccGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAgg < 6:53034/114‑1 (MQ=255) ccGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGAc > 3:99569/1‑133 (MQ=40) ccGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGAc < 4:99569/133‑1 (MQ=40) ccGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTc > 2:71165/1‑149 (MQ=39) cGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTc > 5:178416/1‑148 (MQ=39) aaGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTac < 3:63294/149‑1 (MQ=39) aaGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTac < 1:163469/149‑1 (MQ=39) aaGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTac < 1:206792/149‑1 (MQ=39) ggcgTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCt < 2:209030/142‑1 (MQ=37) ggcgTAATC‑ACCTACA‑CCGTGAACCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTAcaacaa > 4:45511/1‑149 (MQ=37) aTC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTc < 3:45511/149‑1 (MQ=34) | GCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAG‑AC‑CACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATC‑ACCTACA‑CCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCG > NC_002947/197037‑197329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |