Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,870,850 G→A 9.7% D42N (GAC→AAC)  cobC → adenosylcobinamide‑phosphate synthase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,870,8500GA 9.7% 108.9 / 2.6 41D42N (GAC→AAC) cobCadenosylcobinamide‑phosphate synthase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (25/12);  new base A (2/2);  total (27/14)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGGGGGCCTATATCAATGCTTGAACATGGTGGCCGCCTGTTACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGT  >  NC_002947/1870710‑1870998
                                                                                                                                            |                                                                                                                                                    
ccTGGGGGCCTATATCGATGGTTGACCATGGTGGCCGCCTGTTCCGCGCGGTGCAGCAATACGGGATCCCCCGTGAGCAGTGGCTCGCCCTGTCCGGCGGCAGTGCGCCCTGGCCATTTCCTGTCGCGCCGATTCCCGTCGCCGGCTgg                                                                                                                                              >  2:201904/1‑149 (MQ=255)
         cTATATCAATGCTTGAACATGGTGGCCGCCTGTTACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCAACGCCTGGGCCCGCCTg                                                                                                                                     >  3:240003/1‑149 (MQ=255)
         cTATATCAATGCTTGAACATGGTGGCCGCCTGTTACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCAACGCCTGGGCCCGCCTg                                                                                                                                     >  3:235311/1‑149 (MQ=255)
           atatCAATGCTTGAACATGGTGGCCGCCTGTTACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGcc                                                                                                                                   >  2:22071/1‑149 (MQ=255)
                       gAACATGGTGGCCGCCTGTTACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGa                                                                                                                       >  8:237017/1‑149 (MQ=255)
                                   cgccTGTTACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTggagga                                                                                                           >  3:24933/1‑149 (MQ=255)
                                   cgccTGTTACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCACGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTggagga                                                                                                           >  4:221278/1‑149 (MQ=255)
                                       tGTTACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGAcgcc                                                                                                       >  2:25583/1‑149 (MQ=255)
                                        gTTACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGAcgccg                                                                                                      >  4:248907/1‑149 (MQ=255)
                                          tACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCcgcg                                                                                                    >  2:369577/1‑149 (MQ=255)
                                                       gcaATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACg                                                                                       <  1:201904/149‑1 (MQ=255)
                                                         actACGGTAGCCCCCGTGAGCAGTGGCTCGACCTGGCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCAACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGgc                                                                                     <  4:240003/147‑1 (MQ=255)
                                                         aaTACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTCGGCCCGCCTGCCGGAAACCGAGGACGGCCTggag                                                                                                             >  6:13174/1‑125 (MQ=255)
                                                         aaTACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCAACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGgc                                                                                     <  4:235311/149‑1 (MQ=255)
                                                                    ccccGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGtt                                                                          >  8:44349/1‑149 (MQ=255)
                                                                    ccccGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGtt                                                                          >  5:172936/1‑149 (MQ=255)
                                                                      ccGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTtgc                                                                        >  5:100081/1‑149 (MQ=255)
                                                                           aGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGcc                                                                    <  6:172936/148‑1 (MQ=255)
                                                                                  gCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGCCGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCg                                                            >  6:275789/1‑149 (MQ=255)
                                                                                  gCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCg                                                            >  6:221729/1‑149 (MQ=255)
                                                                                    tCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACg                                                                                                                     >  6:192888/1‑90 (MQ=255)
                                                                                           gTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAg                                                    <  1:337739/148‑1 (MQ=255)
                                                                                           gTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAg                                                    >  2:337739/1‑148 (MQ=255)
                                                                                                 cGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGa                                             <  6:100081/149‑1 (MQ=255)
                                                                                                      ttGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAg                                        <  5:221729/149‑1 (MQ=255)
                                                                                                       tGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAgg                                       >  1:199273/1‑149 (MQ=255)
                                                                                                              tGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGcc                                >  2:138571/1‑149 (MQ=255)
                                                                                                              tGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGcc                                >  4:282878/1‑149 (MQ=255)
                                                                                                                gCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCgc                              <  1:22071/149‑1 (MQ=255)
                                                                                                                 ccATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCgct                             <  1:25583/149‑1 (MQ=255)
                                                                                                                  cATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCgctg                            >  2:108139/1‑149 (MQ=255)
                                                                                                                       ccTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCGCtgctgc                        <  3:221278/148‑1 (MQ=255)
                                                                                                                       ccTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCg                       >  7:130029/1‑149 (MQ=255)
                                                                                                                        cTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACTCCGCGCGCGCGTATTACGGCGCCCTCCAGTTGCTGCCGGTAGCCGGCTCCCCGGCCGCGATCCAGGCCTTGCCGCTGCTGCg                       >  8:138373/1‑148 (MQ=255)
                                                                                                                        cTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCg                       >  2:262772/1‑148 (MQ=255)
                                                                                                                        cTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCg                       <  1:369577/148‑1 (MQ=255)
                                                                                                                        cTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCg                       >  3:204199/1‑148 (MQ=255)
                                                                                                                           tCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCGTa                     <  2:199273/147‑1 (MQ=255)
                                                                                                                                ccgATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGcc                                                 >  5:3601/1‑114 (MQ=255)
                                                                                                                                ccgATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGcc                                                 <  6:3601/114‑1 (MQ=255)
                                                                                                                                   aTTGCCGTCGACTCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGAGCCCGCGAGTTGCTGCCGGTAGCCGTCTCCCAGGCCGCGATCCAGGCGTTGCCGCTGCTGCGTACTAGCGccct           <  4:24933/149‑2 (MQ=255)
                                                                                                                                            gACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGt  <  3:248907/149‑1 (MQ=255)
                                                                                                                                            gACGCCTGGGCCCGCCGGCCGGAAACCGCGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGTTGGGCGt  >  4:340113/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
CCTGGGGGCCTATATCAATGCTTGAACATGGTGGCCGCCTGTTACGCGCGGTGCAGCAATACGGTATCCCCCGTGAGCAGTGGCTCGACCTGTCCAGCGGCATTGCGCCCTGGCCATTTCCTGTCCCGCCGATTCCCGTCGACGCCTGGGCCCGCCTGCCGGAAACCGAGGACGGCCTGGAGGACGCCGCGCGCGCGTATTACGGCGCCCGCCAGTTGCTGCCGGTAGCCGGCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGT  >  NC_002947/1870710‑1870998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑