Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,884,979	C→T	6.4%	A121V (GCG→GTG)	PP_1692 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,884,979	0	C	T	6.4%	281.4 / 6.1	93	A121V (GCG→GTG)	PP_1692	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (46/41);  new base T (3/3);  total (49/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CGAGATTGTGGCAACGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTACCCGCGAATACGGT  >  NC_002947/1884831‑1885125                                                                                                                                                        |                                                                                                                                                    cGAGATTGTGGCAACGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGc                                                                                                                                                     <  7:256392/149‑1 (MQ=255)  gagaTTGTGGCAACGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCg                                                                                                                                                    >  8:13225/1‑149 (MQ=255)     aTTGTGGCAACGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCgg                                                                                                                                                   >  5:186550/1‑147 (MQ=255)           gCAACGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCggcagggcag                                                                                                                                           >  7:65834/1‑149 (MQ=255)           gCAACGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCggcagggcag                                                                                                                                           >  7:336437/1‑149 (MQ=255)            cAACGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGgcagggcagg                                                                                                                                          >  6:1884/1‑149 (MQ=255)             aaCGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGcagggcaggg                                                                                                                                         <  5:317195/149‑1 (MQ=255)             aaCGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGcagggcaggg                                                                                                                                         <  4:153128/149‑1 (MQ=255)             aaCGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGCCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GTCTTGAGCTTGCGGcagggcaggg                                                                                                                                         >  4:285815/1‑149 (MQ=255)                  ccGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCg                                                                                                                                     >  1:120461/1‑148 (MQ=255)                  ccGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCg                                                                                                                                     >  3:219454/1‑148 (MQ=255)                         cGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAgc                                                                                                                             >  6:116459/1‑149 (MQ=255)                          ggCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAgcg                                                                                                                            <  2:120461/149‑1 (MQ=255)                           gCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCCCAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGTGGCAGGGCAGGGGTCGCAGGAAgcgc                                                                                                                           >  5:268127/1‑149 (MQ=255)                           gCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGTGGCAGGGCAGGGGTCGCAGGAAgcgc                                                                                                                           >  5:273260/1‑149 (MQ=255)                            cGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGcgcg                                                                                                                          <  5:170905/149‑1 (MQ=255)                                   gCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACATAGTTCCATTGCAAGAGGGCGATTTGGGATAGAGATTGCGGCTGGGCGGGGGTCGCAGGAAGCgcggcggt                                                                                                                     >  1:303584/1‑147 (MQ=255)                                     cATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCtt‑gtt                                                                                                                >  6:219003/1‑149 (MQ=255)                                             gctgcGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGt                                                                                                        >  1:41356/1‑149 (MQ=255)                                              ctgcGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTg                                                                                                       <  7:340722/149‑1 (MQ=255)                                                   tCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGTGAAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGttt                                                                                                   >  1:101154/1‑149 (MQ=255)                                                   tCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGtttt                                                                                                  <  7:13225/149‑1 (MQ=255)                                                     aGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTtct                                                                                                >  5:149015/1‑149 (MQ=255)                                                       gCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGTGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTtctgt                                                                                              <  6:273260/149‑1 (MQ=255)                                                       gCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGTGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTtctgt                                                                                              <  6:268127/149‑1 (MQ=255)                                                       gCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTtctgt                                                                                              >  2:126044/1‑149 (MQ=255)                                                         tGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGa                                                                                                            <  4:299675/133‑1 (MQ=255)                                                         tGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGa                                                                                                            >  3:299675/1‑133 (MQ=255)                                                           cccGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTtctgtct                                                                                            <  5:219003/147‑1 (MQ=255)                                                           cccGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGACGGAGCAGGCGCTCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGa                                                                                          <  2:139871/149‑1 (MQ=255)                                                           cccGCGCAGGGGATCGGCGGGCGCAGCGGGAGTACGCCCGGCGGTGG‑CAGCACAACGCGCCGGAGCAGGCGCGCTCTT‑‑GGCTGGAGCTTGCGGCAGGGCATGGGGCGCAGGAAGCGCGGCGCGT‑GTTGCGACAGTGGTTTTCTGTCTGa                                                                                          <  6:285194/149‑1 (MQ=255)                                                                  aGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCttttt                                                                                   <  8:336437/149‑1 (MQ=255)                                                                      gATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGcc                                                                               >  6:240264/1‑149 (MQ=255)                                                                       aTCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCt                                                                              <  1:126044/149‑1 (MQ=255)                                                                          ggcgggcgCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGc                                                                            <  6:186550/148‑1 (MQ=255)                                                                           gcgggcgCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCtttttt                                                                                  >  3:72097/1‑141 (MQ=255)                                                                           gcgggcgCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCtttttt                                                                                  <  4:72097/141‑1 (MQ=255)                                                                            cgggcgCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACg                                                                         <  3:294740/149‑1 (MQ=255)                                                                                   aGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCtt                                                                  >  7:117504/1‑149 (MQ=255)                                                                                    gCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCttt                                                                 >  6:304044/1‑149 (MQ=255)                                                                                    gCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCttt                                                                 >  8:213951/1‑149 (MQ=255)                                                                                      tGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTcg                                                               >  7:8909/1‑149 (MQ=255)                                                                                      tGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTcg                                                               >  2:288497/1‑149 (MQ=255)                                                                                         aGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCATGCACGGGCCCTTTcgc                                                              >  6:257636/1‑147 (MQ=255)                                                                                          gTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAg                                                           >  4:167357/1‑149 (MQ=255)                                                                                           tACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAg                                                           <  6:48785/148‑1 (MQ=255)                                                                                            aCGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCa                                                                                                                                   <  5:23355/76‑1 (MQ=255)                                                                                            aCGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCa                                                                                                                                   >  6:23355/1‑76 (MQ=255)                                                                                              gCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCaa                                                        >  2:19332/1‑148 (MQ=255)                                                                                              gCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAAt                                                       >  1:203769/1‑149 (MQ=255)                                                                                                ccGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCt                                                                              >  1:287184/1‑124 (MQ=255)                                                                                                ccGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCt                                                                              <  2:287184/124‑1 (MQ=255)                                                                                                ccGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCa                                                     >  5:340568/1‑149 (MQ=255)                                                                                                 cGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCAc                                                    <  5:1884/149‑1 (MQ=255)                                                                                                     tgtgT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGAt                                                <  8:65834/149‑1 (MQ=255)                                                                                                       tgt‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGAtgt                                              <  7:213951/149‑1 (MQ=255)                                                                                                       tgt‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGAtgt                                              <  1:288497/149‑1 (MQ=255)                                                                                                       tgt‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGAtgt                                              <  4:219454/149‑1 (MQ=255)                                                                                                         t‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGa                                                 <  7:20429/144‑1 (MQ=255)                                                                                                         t‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGa                                                 >  8:20429/1‑144 (MQ=255)                                                                                                            aGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GTCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGAtgtgtg                                           <  3:285815/148‑1 (MQ=255)                                                                                                            aGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATgtgtgt                                          >  2:265940/1‑149 (MQ=255)                                                                                                             gCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTc                                         >  3:193213/1‑149 (MQ=255)                                                                                                              cacaACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTc                                         >  4:232244/1‑148 (MQ=255)                                                                                                               acaACGAGCCGGATCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCa                                       <  2:101154/149‑1 (MQ=255)                                                                                                               acaACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCa                                       <  4:193213/149‑1 (MQ=255)                                                                                                                caACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCAc                                      <  2:41356/149‑1 (MQ=255)                                                                                                                 aaCGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCAcc                                     >  4:140298/1‑149 (MQ=255)                                                                                                                 aaCGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCAcc                                     >  1:234278/1‑149 (MQ=255)                                                                                                                 aaCGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCAcc                                     >  2:92454/1‑149 (MQ=255)                                                                                                                 aaCGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCAcc                                     >  2:33498/1‑149 (MQ=255)                                                                                                                    gAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCAccgcc                                  >  5:287753/1‑149 (MQ=255)                                                                                                                       ccGGATCAGTCTCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTGTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTg                               <  2:234278/149‑1 (MQ=255)                                                                                                                        cGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTgg                              <  5:304044/149‑1 (MQ=255)                                                                                                                        cGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTgg                              <  6:149015/149‑1 (MQ=255)                                                                                                                           aGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAg                           >  5:231210/1‑149 (MQ=255)                                                                                                                                 cgcgcTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCAGCGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCgg                     <  1:92454/149‑1 (MQ=255)                                                                                                                                 cgcgcTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCgg                     <  6:231210/149‑1 (MQ=255)                                                                                                                                 cgcgcTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCgg                     <  1:297866/149‑1 (MQ=255)                                                                                                                                  gcgcTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCggg                    <  5:240264/149‑1 (MQ=255)                                                                                                                                      tATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGa                                                            <  7:318167/105‑1 (MQ=255)                                                                                                                                      tATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGa                                                            >  8:318167/1‑105 (MQ=255)                                                                                                                                        tt‑‑GGCTTGAGCTTGCGGCAGGGCAGTCGTCGCAGGAAGCGCGGCGATT‑GTTGCGACAGTGGTTTTCTGTATGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTATAcc              >  8:31553/1‑149 (MQ=255)                                                                                                                                        tt‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTTGGTTGGGAATTTGGTTTTCTTTGTGAGATTTTTTGCCTGCACGGGCCGTTTCGGGAGCAATCACCGATGTTTGTCCACCGCCGTGTTAGGCGCGGGTTTAc               >  1:272144/1‑149 (MQ=255)                                                                                                                                        tt‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTAcc              >  5:137934/1‑149 (MQ=255)                                                                                                                                        tt‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTAc               <  1:265940/148‑1 (MQ=255)                                                                                                                                            ggCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTAccc             <  6:137934/148‑1 (MQ=255)                                                                                                                                              cTTGAGCTTGTGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTACCcgc           >  1:137773/1‑148 (MQ=255)                                                                                                                                              cTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTACCcgc           >  1:364794/1‑148 (MQ=255)                                                                                                                                                 gAGCTTGTGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTACCCGCGaa        <  2:137773/148‑1 (MQ=255)                                                                                                                                                   gCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTACCCGCGAATAc     >  7:280120/1‑149 (MQ=255)                                                                                                                                                    cTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTACCCGCGAATACg    <  6:340568/149‑1 (MQ=255)                                                                                                                                                       gCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTACCCGCGAATACGGt  <  2:303584/148‑1 (MQ=255)                                                                                                                                                       gCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTACCCGCGAATACGGt  <  8:280120/148‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                    CGAGATTGTGGCAACGCCCGTGACCGGCGTATAGGCCATGAGCTGCTGCGTCAGGCTGCCCGCGCAGGGGATCGGCGGGCGCAGCTGGAGTACGCCCGGCTGTGT‑CAGCACAACGAGCCGGAGCAGGCGCGCTATT‑‑GGCTTGAGCTTGCGGCAGGGCAGGGGTCGCAGGAAGCGCGGCGCTT‑GTTGCGACAGTGGTTTTCTGTCTGAGCTTTTTTGCCTGCACGGGCCCTTTCGCGAGCAATCACCGATGTGTGTCCACCGCCGTGGTAGGCGCGGGTTTACCCGCGAATACGGT  >  NC_002947/1884831‑1885125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑