Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 197,551 C→G 17.4% T1019T (ACC→ACG)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947197,5510CG17.4% 276.8 / 43.1 119T1019T (ACC→ACG) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (51/47);  new base G (9/12);  total (60/59)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGAC  >  NC_002947/197406‑197694
                                                                                                                                                    |                                                                                                                                               
ccACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCAtt                                                                                                                                              <  1:319778/149‑1 (MQ=37)
ccACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCAtt                                                                                                                                              <  6:163602/149‑1 (MQ=37)
 cACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTa                                                                                                                                             <  5:166930/149‑1 (MQ=37)
    gATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCg                                                                                                                                          >  5:93666/1‑149 (MQ=37)
      tCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGtt                                                                                                                                        >  2:35402/1‑149 (MQ=37)
        aaCGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTg                                                                                                                                       <  3:194817/148‑1 (MQ=37)
           gACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGc                                                                                                                                   >  7:324839/1‑149 (MQ=37)
                        ccaccaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAg                                                                                                                      <  1:78839/149‑1 (MQ=37)
                         caccacCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCACAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGa                                                                                                                                      <  1:92161/133‑1 (MQ=255)
                         caccacCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAgg                                                                                                                     >  2:292296/1‑149 (MQ=37)
                          accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAggg                                                                                                                    >  8:128045/1‑149 (MQ=37)
                          accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAggg                                                                                                                    >  2:16495/1‑149 (MQ=37)
                                gcgACCCTGACGGCGAGCGCGTCGGTCACCGAAGGGGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCTGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGt                                                                                                              <  3:291411/149‑1 (MQ=255)
                                gcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCATGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGt                                                                                                              >  4:45381/1‑149 (MQ=37)
                                 cgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                 <  6:315948/129‑1 (MQ=38)
                                 cgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                 >  5:315948/1‑129 (MQ=38)
                                       tGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTc                                                                                                       >  4:180027/1‑149 (MQ=37)
                                       tGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTc                                                                                                       >  1:218157/1‑149 (MQ=37)
                                         aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTcc                                                                                                      <  1:16495/148‑1 (MQ=37)
                                           ggCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGa                                                                                                   >  4:118467/1‑149 (MQ=37)
                                                  ccGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCgg                                                                                                                                <  5:275122/113‑1 (MQ=39)
                                                  ccGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCgg                                                                                                                                >  6:275122/1‑113 (MQ=39)
                                                   cGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGc                                                                                            >  8:128093/1‑148 (MQ=37)
                                                       ggTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAgg                                                                                                                     <  2:247731/119‑1 (MQ=39)
                                                       ggTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAgg                                                                                                                     >  1:247731/1‑119 (MQ=39)
                                                        gTCACCGAAGGCGGCGTGATCACCTACACAGTGATCCTGAACAATCCTGCC‑CAGACGCCTGTAACCGTGACCCTGTCTCGTACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                        <  1:299522/149‑1 (MQ=11)
                                                          cACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTcc                                                                                                      <  8:14688/131‑1 (MQ=37)
                                                          cACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTcc                                                                                                      >  7:14688/1‑131 (MQ=37)
                                                           aCCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAgg                                                                                                                     >  1:4624/1‑115 (MQ=39)
                                                           aCCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGt                                                                                   >  4:249320/1‑149 (MQ=37)
                                                           aCCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGt                                                                                   >  7:45870/1‑149 (MQ=37)
                                                              gAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCt                                                                                 >  6:250773/1‑148 (MQ=37)
                                                                 ggcggcGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa                                                                             >  4:174442/1‑149 (MQ=37)
                                                                    ggcgTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTAcaacaa                                                                          <  5:250773/149‑1 (MQ=37)
                                                                      cgTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACg                                                                        <  2:259192/149‑1 (MQ=37)
                                                                          aTCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt                                                                     <  4:301260/148‑1 (MQ=37)
                                                                          aTCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt                                                                     <  1:292296/148‑1 (MQ=37)
                                                                          aTCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt                                                                     <  3:118467/148‑1 (MQ=37)
                                                                              ccTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCa                                                                                                     >  5:32826/1‑112 (MQ=39)
                                                                              ccTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGCATGACGTCTACAACAACGGTTCGACt                                                                <  1:198472/149‑1 (MQ=38)
                                                                              ccTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGAcc                                                                <  2:218157/149‑2 (MQ=25)
                                                                              ccTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACt                                                                <  3:155582/149‑1 (MQ=38)
                                                                               cTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAATCGTGACCCTGTC‑C‑AACGGCCAAACGATTACCGTTGAAGCCGGCAACACCCAGGGCAGCGTCGACTTCCAGa                                                                                                   >  5:290800/1‑113 (MQ=14)
                                                                               cTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAATCGTGACCCTGTC‑C‑AACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGa                                                                                                   <  6:290800/113‑1 (MQ=11)
                                                                               cTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCCAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTa                                                                                >  4:223348/1‑132 (MQ=37)
                                                                               cTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTa                                                                                <  3:223348/132‑1 (MQ=37)
                                                                               cTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa                                                                  >  3:219175/1‑146 (MQ=37)
                                                                               cTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AAAGGCCAACCCATTACCGTTGAAGCCGGCAAGACCCCGGCCAGCCTCGATTTCCAGACCCCGGCGAATGAAGTCTACAACAACGGTTCGCCt                                                                >  7:269943/1‑148 (MQ=11)
                                                                                     cGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑G‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAGTTCCAGACCCCGGCGAATGACGTCTACAACAACCGTTCGACCGTCAGCg                                                         >  1:86352/1‑149 (MQ=255)
                                                                                      cgatcgcTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGt                                                        <  3:67678/143‑1 (MQ=255)
                                                                                       tGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGt                                                        >  6:227647/1‑148 (MQ=39)
                                                                                       tGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTc                                                       <  3:180027/149‑1 (MQ=21)
                                                                                         aTCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCa                                                      <  2:164748/148‑1 (MQ=38)
                                                                                          tCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCAc                                                     <  1:35402/148‑1 (MQ=17)
                                                                                          tCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCAGGCGAATGACGTCTACAACAACGGGTCGACCGTCAGCGTCAc                                                     >  6:12239/1‑148 (MQ=12)
                                                                                           ccTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCa                                                   >  1:5695/1‑149 (MQ=37)
                                                                                            cTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCa                                                   <  5:227647/148‑1 (MQ=37)
                                                                                             tGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc                                                 >  5:236867/1‑149 (MQ=37)
                                                                                             tGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACACCGGCGAAAGACGTCTACAACAACGGTTCGACTGTCAGctt                                                        >  4:67678/1‑140 (MQ=255)
                                                                                             tGAGCAATCCTGCC‑CAGACGCCGGAAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc                                                 >  2:231231/1‑149 (MQ=37)
                                                                                             tGAGCAATCCTCCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGCAGTCTACAACAACGGTTCGACCGTCAGCGTCACCATc                                                 >  8:270256/1‑149 (MQ=255)
                                                                                               aGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc                                                 <  7:128045/147‑1 (MQ=37)
                                                                                               aGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATc                                                 >  8:332077/1‑147 (MQ=17)
                                                                                                gCAATCCTGCC‑CAGACGCCGGTAACCGTGCCCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCAGGCGAATGACGTCTACAACAAAGGTTCGACTCTCAGCGTCACCATc                                                 >  5:173916/1‑146 (MQ=255)
                                                                                                gCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc                                                 >  2:17040/1‑146 (MQ=37)
                                                                                                  aaTCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTa                                                                                >  6:73024/1‑113 (MQ=35)
                                                                                                  aaTCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTa                                                                                <  5:73024/113‑1 (MQ=35)
                                                                                                      cTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGGCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACAAACGAGAACGcc                                        >  6:380428/1‑149 (MQ=255)
                                                                                                         cc‑cAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCca                                       <  3:174442/147‑1 (MQ=17)
                                                                                                          c‑cAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCa                                                      <  3:244544/131‑1 (MQ=37)
                                                                                                          c‑cAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACACCGGCGAATGACGTCTACAACAAAGCTTCGACTGTCAGCGTCa                                                      >  4:244544/1‑131 (MQ=255)
                                                                                                          c‑cAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTACCAGACCCCGGCGAATGACCTCAACAACAACCCTTCGACTGTCAGCGTCACAATCGAGAACGCCACAg                                    >  5:227043/1‑149 (MQ=11)
                                                                                                            cAGACGCCGGTGACCGTGACCCTGTC‑C‑AACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAgg                                   <  7:24274/149‑1 (MQ=255)
                                                                                                            cAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAgg                                   <  7:332077/149‑1 (MQ=17)
                                                                                                              gACGCCGGTGACCGTGACCCTGTC‑C‑AACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCg                                 <  2:86146/149‑1 (MQ=255)
                                                                                                              gACGCCGGTGACCGTGACCCTGTC‑C‑AACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGGCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCg                                 <  3:38349/149‑1 (MQ=11)
                                                                                                              gACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCg                                 <  7:128093/149‑1 (MQ=17)
                                                                                                                 gCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTa                              <  8:2453/149‑1 (MQ=17)
                                                                                                                  ccGGTGACCGTGACCCTGTC‑C‑AACGGCCAAAGGATTACCGTTGAAGCCGGCAAAACGCAGGGCAGCGTCGACTTCCAGACCCCGGAGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCAGCATCGAGAACGCCACAGGCGGTaa                             <  8:16490/149‑1 (MQ=11)
                                                                                                                  ccGGTGACCGTGACCCTGTC‑C‑AACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTaa                             <  5:203739/149‑1 (MQ=255)
                                                                                                                  ccGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCCTCGAGAACGCCACAGGCGGTaa                             <  7:231700/149‑1 (MQ=37)
                                                                                                                      tgaCCGTGACCCTGTC‑C‑AACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTc                         <  4:261225/147‑1 (MQ=255)
                                                                                                                      tAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTc                         <  2:290797/149‑1 (MQ=35)
                                                                                                                        aCCGTGACCATGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa                       <  3:45381/149‑1 (MQ=21)
                                                                                                                         ccGTGACCCTGTC‑C‑AACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCg                                                <  2:45954/123‑1 (MQ=9)
                                                                                                                            tGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCaa                   <  6:110139/149‑1 (MQ=32)
                                                                                                                              aCCCTGTC‑C‑AACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACt                 >  7:336694/1‑149 (MQ=12)
                                                                                                                                ccTGTC‑C‑AACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACt                 >  1:316258/1‑147 (MQ=12)
                                                                                                                                ccTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGa               >  4:152306/1‑149 (MQ=32)
                                                                                                                                    tC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAcc             <  1:17040/147‑1 (MQ=32)
                                                                                                                                       c‑AACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAgg                                   <  6:336091/123‑1 (MQ=12)
                                                                                                                                         aaCGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTc                         >  7:322020/1‑132 (MQ=12)
                                                                                                                                         aaCGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTc                         <  8:322020/132‑1 (MQ=12)
                                                                                                                                          aCGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAc       <  2:177979/149‑1 (MQ=12)
                                                                                                                                          aCGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAc       >  4:98879/1‑149 (MQ=32)
                                                                                                                                            ggCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACCGATCGACTGTCAGCGTCACCAACCAGAACGCCCCAGGCGGTAAATTCGAGCAACTGACCCCGCAccc     >  2:119645/1‑149 (MQ=11)
                                                                                                                                             gCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAccc     >  2:338301/1‑148 (MQ=255)
                                                                                                                                               cAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGAc  >  6:361860/1‑149 (MQ=255)
                                                                                                                                                   ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa                       >  4:190803/1‑124 (MQ=32)
                                                                                                                                                   ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa                       <  3:190803/124‑1 (MQ=32)
                                                                                                                                                    |                                                                                                                                               
CCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCC‑CAGACGCCGGTAACCGTGACCCTGTC‑C‑AACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGAC  >  NC_002947/197406‑197694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑