Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,960,867 G→A 9.1% V231I (GTC→ATC)  PP_1758 → rhodanese‑like domain‑containing sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,960,8670GA 9.1% 121.7 / 2.9 44V231I (GTC→ATC) PP_1758rhodanese‑like domain‑containing sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base G (20/20);  new base A (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

ATCCGTTGCGAAAAAGCCTCCAGCTACATGCTCGGTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCAGCTGCCCGCATTGCTGGGACAGCCTGAGCGAGAAGAC  >  NC_002947/1960720‑1961012
                                                                                                                                                   |                                                                                                                                                 
aTCCGTTGCGAAAAAGCCTCCAGCTACATGCTCGGTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGt                                                                                                                                                  <  1:349907/149‑1 (MQ=255)
     ttGCGAAAAAGCCTCCAGCTACATGCTCGGTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACgg                                                                                                                                             <  1:72076/149‑1 (MQ=255)
        cGAAAAAGCCTCCAGCTACATGCTCGGTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGc                                                                                                                                          >  2:113828/1‑149 (MQ=255)
         gAAAAAGCCTCCAGCTACATGCTCGGTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCg                                                                                                                                         >  4:70895/1‑149 (MQ=255)
          aaataGCCTCCATCTACTTGCTCGTTGATGGCTTCGATTCTGGCTATCTTCTTAAGGGCGGCATCCTGAAATACTTCGATGTATTGCCTCAGGAAGAAAGCCTGTGGGACGGCTACTGCTTCGTCTTCTACAACCGGGTCACGGTGCGt                                                                                                                                        <  4:238108/149‑1 (MQ=255)
                           aTGATCGGTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAgg                                                                                                                       >  3:64474/1‑149 (MQ=255)
                            tGCTCGGTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGc                                                                                                                      <  8:73251/149‑1 (MQ=255)
                              cTCGGTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGa                                                                                                                    <  2:50430/149‑1 (MQ=255)
                                 ggTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGt                                                                                                                  <  1:113828/148‑1 (MQ=255)
                                 ggTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGt                                                                                                                  <  3:70895/148‑1 (MQ=255)
                                          ttCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTg                                                                                                        >  3:360332/1‑149 (MQ=255)
                                                   gTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTg                                                                                               >  2:338876/1‑149 (MQ=255)
                                                         catcTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCa                                                                                         >  1:177040/1‑149 (MQ=255)
                                                           tcTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTc                                                                                                                                       <  6:157966/101‑1 (MQ=255)
                                                           tcTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTc                                                                                                                                       >  5:157966/1‑101 (MQ=255)
                                                              tAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGa                                                                                    <  8:247610/149‑1 (MQ=255)
                                                               aaGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGAt                                                                                   >  3:258105/1‑149 (MQ=255)
                                                                agtgcggcATCCTGAAATACCTCTCGCAAGTGCCTCCGGAAGAAAGCCTGTGGGACGCCGACTCCATCGTCTTCGACAACCGGGTCACCGGGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGAt                                                                                   <  6:117123/145‑1 (MQ=255)
                                                                   gcggcATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAAt                                                                               <  4:64474/149‑1 (MQ=255)
                                                                       cATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGcac                                                                           <  1:338876/149‑1 (MQ=255)
                                                                         tCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAg                                                                         <  4:360332/149‑1 (MQ=255)
                                                                          ccTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAACGCGAGTAGGACCAGTGCCACGCCTGCCGCCATCCGATAAATGCACAg                                                                         >  2:109312/1‑148 (MQ=255)
                                                                                    ttCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGCCTGCTTCGTCTTCGACAACCGGATCGCGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTc                                                              >  2:268550/1‑149 (MQ=255)
                                                                                    ttCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTc                                                              >  1:353910/1‑149 (MQ=255)
                                                                                    ttCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGATCGCGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCACGCCGAGCACTGCACAGGCGCGCGCGTc                                                              >  2:263461/1‑149 (MQ=255)
                                                                                                 cTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGCCCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCg                                                 >  4:232935/1‑149 (MQ=255)
                                                                                                    atgaagaaAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCa                                              <  2:150095/147‑1 (MQ=255)
                                                                                                     ggAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAg                                             >  2:274033/1‑149 (MQ=255)
                                                                                                     ggAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCATCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAg                                             >  6:209456/1‑149 (MQ=255)
                                                                                                      gaagaaAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAgg                                            >  5:372280/1‑149 (MQ=255)
                                                                                                       aagaaAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGgt                                           <  1:274033/149‑1 (MQ=255)
                                                                                                         gaaAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGgtgt                                         >  3:291027/1‑149 (MQ=255)
                                                                                                         gaaAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGgtgt                                         >  1:40784/1‑149 (MQ=255)
                                                                                                         gaaAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGgtgt                                         <  4:258105/149‑1 (MQ=255)
                                                                                                           aaGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATGAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCa                                       <  1:109312/149‑1 (MQ=255)
                                                                                                           aaGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCa                                       <  4:329987/149‑1 (MQ=255)
                                                                                                              ccTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCAGCt                                    >  2:341123/1‑149 (MQ=255)
                                                                                                                           gACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCAGCTGCCCGCATTGCTg                       <  4:291027/149‑1 (MQ=255)
                                                                                                                                cTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCAGCTGCCCGCATTGCTGGGACa                  <  1:341123/149‑1 (MQ=255)
                                                                                                                                 ttCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCAGCTGCCCGCATTGCTGGGACAg                 >  1:7232/1‑149 (MQ=255)
                                                                                                                                     tCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCAGCTGCCCGCATTGCTGGGACAGCCt              >  1:86391/1‑148 (MQ=255)
                                                                                                                                         cGACAACCGGATCGCGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCAGCTGCCCGCATTGCTGGGACAGCCTGAGc          <  1:263461/148‑1 (MQ=255)
                                                                                                                                         cGACAACCGGATCGCGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCAGCTGCCCGCATTGCTGGGACAGCCTGAGc          <  1:268550/148‑1 (MQ=255)
                                                                                                                                                cGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCAGCTGCCCGCATTGCTGGGACAGCCTGAGCGAGAAGAc  >  8:256287/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATCCGTTGCGAAAAAGCCTCCAGCTACATGCTCGGTGAAGGCTTCGAGTCGGTCTATCATCTTAAGGGCGGCATCCTGAAATACTTCGAGGAAGTGCCTCAGGAAGAAAGCCTGTGGGACGGCGACTGCTTCGTCTTCGACAACCGGGTCACGGTGCGTCATGACCTGAGCGAAGGCGAGTACGACCAGTGCCACGCCTGCCGCCATCCGATCAATGCACAGGAGCGCGCGTCCGAGCACTATTCGCCAGGTGTCAGCTGCCCGCATTGCTGGGACAGCCTGAGCGAGAAGAC  >  NC_002947/1960720‑1961012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑