Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,973,252 T→G 7.0% I727M (ATT→ATG)  gyrA → DNA gyrase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,973,2520TG 7.0% 174.5 / 4.6 57I727M (ATT→ATG) gyrADNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (27/26);  new base G (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

CCGGAGTGGGTTGCAGAGCCGACCGGCGCGTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGCTGGGCAAAGGCCAGCGGAT  >  NC_002947/1973106‑1973398
                                                                                                                                                  |                                                                                                                                                  
ccGGAGTGGGTTGCAGAGCCGACCGGCGCGTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGc                                                                                                                                                  <  3:226555/149‑1 (MQ=255)
      tGGGTTGCAGAGCCGACCGGCGCGTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGc                                                                                                                                            >  3:326080/1‑149 (MQ=255)
       gggTTGCAGAGCCGACCGGCGCGTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGc                                                                                                                                            >  1:71511/1‑148 (MQ=255)
             cAGAGCCGACCGGCGCGTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATGGCcgc                                                                                                                                               <  1:112757/139‑1 (MQ=255)
             cAGAGCCGACCGGCGCGTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATGGCcgc                                                                                                                                               >  2:112757/1‑139 (MQ=255)
                agCCGACCGGCGCGTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGAt                                                                                                                                  <  4:14703/149‑1 (MQ=255)
                agCCGACCGGCGCGTACATCTTCATGGCGACCGCTTCCGGTACCGGCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAACCTGAAGGAACGAGACACCCTGATTGCCGCGGCCATCACCGAt                                                                                                                                  >  3:131172/1‑149 (MQ=255)
                 gCCGACCGGCGCGTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATg                                                                                                                                 <  5:36250/149‑1 (MQ=255)
                          cgcgTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAgg                                                                                                                        <  3:1256/149‑1 (MQ=255)
                            cgTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGaa                                                                                                                      <  3:63912/149‑1 (MQ=255)
                             gTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAg                                                                                                                     >  6:293073/1‑149 (MQ=255)
                                 aTCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGaa                                                                                                                      >  3:233549/1‑144 (MQ=255)
                                 aTCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGaa                                                                                                                      <  4:233549/144‑1 (MQ=255)
                                  tCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCACTTCGCCCGTCCGCGCTCCACCGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCat                                                                                                                 >  7:50223/1‑148 (MQ=255)
                                      cATGGCGACCGCTTCCTGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCatg                                                                                                                <  1:23337/145‑1 (MQ=255)
                                           cGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTcc                                                                                                       <  2:343255/149‑1 (MQ=255)
                                           cGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTcc                                                                                                       >  7:205804/1‑149 (MQ=255)
                                             aCCGCTTCCGGTACCGTAATGAAGACCAGTCGGGTGCAGGTCGCCCGTCCTCGCGCCAACGGACTTATTGCCCTGAAGATGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGa                                                                                                     <  4:238994/149‑1 (MQ=255)
                                              ccGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGAc                                                                                                    >  5:17907/1‑149 (MQ=255)
                                                gCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGc                                                                                                  >  5:119574/1‑149 (MQ=255)
                                                       gTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCGCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAg                                                                                           >  4:125303/1‑149 (MQ=255)
                                                       gTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAg                                                                                           >  1:313037/1‑149 (MQ=255)
                                                             tCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCAtt                                                                                     >  3:196040/1‑149 (MQ=255)
                                                               aagaagACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCg                                                                                   >  2:129021/1‑149 (MQ=255)
                                                               aagaagACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCg                                                                                   >  8:222443/1‑149 (MQ=255)
                                                                 gaagaCCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCt                                                                                 >  4:42724/1‑149 (MQ=255)
                                                                 gaagaCCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCt                                                                                 >  3:244557/1‑149 (MQ=255)
                                                                 gaagaCCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGCTCATTCgat                                                                                 >  8:262356/1‑147 (MQ=255)
                                                                     aCCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGc                                                                             <  8:205804/149‑1 (MQ=255)
                                                                        ccGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGcc                                                                            >  2:127295/1‑147 (MQ=255)
                                                                          gCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCgaga                                                                        <  3:125303/149‑1 (MQ=255)
                                                                                      cGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTcgtgcgt                                                             >  2:309064/1‑148 (MQ=255)
                                                                                         ccGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGaaa                                                         <  4:326080/149‑1 (MQ=255)
                                                                                          cGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAAt                                                        <  7:222443/149‑1 (MQ=255)
                                                                                            tCCGCGCTCCAACGGCCTGATTGCACTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGGCAATCGCTTTGCCGAGAGCGTCGTGCGTGAAATgg                                                      <  8:50223/149‑1 (MQ=255)
                                                                                               gcgcTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGcc                                                   <  6:119574/149‑1 (MQ=255)
                                                                                                          gCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCgt                                        <  1:127295/149‑1 (MQ=255)
                                                                                                           ccTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCgtg                                       <  2:71511/149‑1 (MQ=255)
                                                                                                           ccTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCgtg                                       <  6:17907/149‑1 (MQ=255)
                                                                                                                    ccTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTCGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGcgcg                              <  3:42724/149‑1 (MQ=255)
                                                                                                                     ctgaagctgaagGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTgcgc                               <  4:358260/147‑1 (MQ=255)
                                                                                                                     ctgaagctgaagGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTgcgc                               >  3:358260/1‑147 (MQ=255)
                                                                                                                        aagctgaagGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCa                           <  1:129021/148‑1 (MQ=255)
                                                                                                                          gctgaagGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATg                         >  4:113129/1‑148 (MQ=255)
                                                                                                                          gctgaagGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCAGCATg                         >  2:143072/1‑148 (MQ=255)
                                                                                                                             gaaggaagGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGc                     >  4:63004/1‑149 (MQ=255)
                                                                                                                             gaaggaagGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGc                     >  1:332276/1‑149 (MQ=255)
                                                                                                                                ggaaggCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCAGCATGAAGCTgg                  <  1:143072/149‑1 (MQ=255)
                                                                                                                                    ggCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGCTGGGCa               >  6:270983/1‑148 (MQ=255)
                                                                                                                                        acacCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGCTGGGCAAAGGc          <  4:196040/149‑1 (MQ=255)
                                                                                                                                         atcggtGATGGCCGCGGCAATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCgtg                                       <  2:82342/114‑1 (MQ=255)
                                                                                                                                         atcggtGATGGCCGCGGCAATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCgtg                                       >  1:82342/6‑119 (MQ=255)
                                                                                                                                            ccTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGCTGGGCAAAGGCCAGc      >  3:170556/1‑149 (MQ=255)
                                                                                                                                            ccTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGCTGGGCAAAGGCCAGc      <  3:113129/149‑1 (MQ=255)
                                                                                                                                                aTTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGCTgg                  <  7:40253/133‑1 (MQ=255)
                                                                                                                                                aTTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGCTgg                  >  8:40253/1‑133 (MQ=255)
                                                                                                                                                aTTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGCTGGGCAAAGGCCAGCGGAt  <  2:313037/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGGAGTGGGTTGCAGAGCCGACCGGCGCGTACATCTTCATGGCGACCGCTTCCGGTACCGTCAAGAAGACCCCGCTGGTGCAGTTCGCCCGTCCGCGCTCCAACGGCCTGATTGCCCTGAAGCTGAAGGAAGGCGACACCCTGATTGCCGCGGCCATCACCGATGGCGCCAAGGAAGTCATGATGTTCTCCGACGCTGGCAAGGTCATTCGCTTTGCCGAGAGCGTCGTGCGTGAAATGGGCCGCAACGCCCGTGGTGTGCGCGGCATGAAGCTGGGCAAAGGCCAGCGGAT  >  NC_002947/1973106‑1973398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑