| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,975,985 | A→G | 7.7% | Y306C (TAT→TGT) ‡ | pheA → | bifunctional chorismate mutase/prephenate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,975,985 | 0 | A | G | 7.7% | 189.8 / 4.9 | 67 | Y306C (TAT→TGT) ‡ | pheA | bifunctional chorismate mutase/prephenate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (32/29); new base G (3/2); total (36/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
AGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTGTGGCG > NC_002947/1975841‑1976133 | agaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCa < 7:191725/148‑1 (MQ=255) gaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTTACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAg > 8:211216/1‑148 (MQ=255) gaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTTACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAg > 8:216890/1‑148 (MQ=255) gaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAg > 8:316835/1‑148 (MQ=255) aaGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCAGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGc < 1:206568/149‑1 (MQ=255) cAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGAccc < 8:116306/149‑1 (MQ=255) cACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTg < 5:136690/139‑1 (MQ=255) cACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTg > 6:136690/1‑139 (MQ=255) cACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATc > 2:224294/1‑148 (MQ=255) gcgcTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCgaga > 5:102509/1‑149 (MQ=255) ttCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTGATAGAACGGCacca < 8:244507/127‑4 (MQ=255) ttCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTGATAGAACGGCacca > 7:244507/1‑124 (MQ=255) ttCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGAcccg > 1:215649/1‑149 (MQ=255) ccTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGAcccgc < 5:316117/148‑1 (MQ=255) cTGATGATCGGTAACCTGGAAGTACCGCCGACCGGCGACGACAAGACCTCCATCATCGTTTCCATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGAcccgcc < 3:2320/148‑1 (MQ=255) tgatgaTCGGTAACCAGGAAGTACCGCCGACCGGAGACGACAAGACCTAGATCATCGTTTCGATGAGCAACAAGACAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGAcccgcccg > 3:146695/1‑149 (MQ=255) gatgatCGGTAACCAGGAAGTACCGCCGACCGGCGCCGACAAGACCTCGATCATCGTTTCGATGAGCACAAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCAGCGACCTGCCCCGCATCGAGACCCGCCCGt > 7:162413/1‑149 (MQ=255) gatgatCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGt > 5:255344/1‑149 (MQ=255) tCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGTTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTcgcg > 3:323647/1‑148 (MQ=255) ggTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTCCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAg > 6:68214/1‑149 (MQ=255) gTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTTTCAGAACGGCATCGCCCTGACCCGCATCGAGACGCGCCCGTCGCGCAGc > 7:273019/1‑149 (MQ=255) cAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGc > 2:17715/1‑147 (MQ=255) ccgccgACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTa > 7:87765/1‑149 (MQ=255) cgccgACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTAc < 2:215649/149‑1 (MQ=255) cgccgACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTAc < 4:61118/149‑1 (MQ=255) cgccgACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTAc < 7:216890/149‑1 (MQ=255) cgccgACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTAc < 7:211216/149‑1 (MQ=255) gACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACgtgt < 6:102509/149‑1 (MQ=255) ccGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGttc < 2:342838/149‑1 (MQ=255) ccGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGttc < 7:316835/149‑1 (MQ=255) ccgacAACCCGTCGATCATCGTTTCGATGAGAAACAAGCCAGCTCCGTTGCAGGAGTGGCTGGTGCCGTGCTATCACCACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATc < 6:213585/148‑1 (MQ=255) gACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTtcg > 2:95629/1‑149 (MQ=255) gACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTtcg > 4:49516/1‑149 (MQ=255) ccTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTtcgtc < 1:224294/149‑1 (MQ=255) cTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTtcgtcg < 8:87765/149‑1 (MQ=255) gTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACcg > 4:221119/1‑149 (MQ=255) gTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACcg > 1:7182/1‑149 (MQ=255) tCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGa > 2:29729/1‑149 (MQ=255) gATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGAcc > 5:79711/1‑149 (MQ=255) gATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGAc < 6:255344/148‑1 (MQ=255) gATGAGCAACAAGCACGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCCTCGAGACCCGCCCGGCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGAc < 8:162413/148‑1 (MQ=255) ccgatctaGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGc < 7:157330/141‑1 (MQ=255) aGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCt > 8:176431/1‑149 (MQ=255) gCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCt > 2:197415/1‑148 (MQ=255) gCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTg < 5:151019/149‑1 (MQ=255) caacaaGCCAGGTGCCTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCtc > 6:307938/1‑147 (MQ=255) aacaaGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACcgc < 6:10893/138‑1 (MQ=255) aacaaGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGAt > 5:194692/1‑149 (MQ=255) aacaaGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGAAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACcgc > 5:10893/1‑138 (MQ=255) gCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGtt < 6:315958/105‑1 (MQ=255) gCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGtt > 5:315958/1‑105 (MQ=255) gCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGc < 4:172619/124‑1 (MQ=255) gCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGc > 8:157330/1‑140 (MQ=255) cATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGc < 7:176431/148‑1 (MQ=255) tGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCAGCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGa < 6:79711/149‑1 (MQ=255) ttGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAg < 1:107047/141‑1 (MQ=255) ttGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAg > 2:107047/1‑141 (MQ=255) gTGCCGTTCTATCAGTACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTAc > 2:13157/1‑75 (MQ=255) gTGCCGTTCTATCAGTACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTAc < 1:13157/75‑1 (MQ=255) tGCCGTTCTGATAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAg > 4:96661/1‑134 (MQ=255) tGCCGTTCTGATAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAg < 3:96661/134‑1 (MQ=255) gCCGTTCTGATAGAACGGCACCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCaggag > 1:131310/1‑148 (MQ=255) gTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCtg < 4:15030/149‑1 (MQ=255) gTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCtg > 3:15030/1‑149 (MQ=255) ttCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAgg > 4:39069/1‑118 (MQ=255) ttCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAgg < 3:39069/118‑1 (MQ=255) ttCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCtgt > 6:190498/1‑149 (MQ=255) aTCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTGTGgcg > 5:181881/1‑149 (MQ=255) | AGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTGTGGCG > NC_002947/1975841‑1976133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |